Ectopic expression of Arabidopsis broad-spectrum resistance gene RPW8.2 improves the resistance to powdery mildew in grapevine (Vitis vinifera)

Yang, Huanming; Li, Yajuan; Hou, Fengjuan; Wan, Dongyan; Cheng, Yuan; Han, Yujun; Gao, Yurong; Liu, Jie; Guo, Yifeng; Xiao, Shaobo; Wang, Yuejin; Wen, Ying-Qiang

doi:10.1016/j.plantsci.2017.11.005

Cited by 32 publications

(24 citation statements)

References 47 publications

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“…R-gene genomic studies frequently neglect to assess the presence of NLR-associated RPW8 domains. Two NBS-RPW8 proteins conferring mildew-resistance have been described in the Arabidopsis thaliana genome (Xiao et al 2001) that retained their function when expressed in grape (Hu et al 2018). The At RPW8.2 gene was recently shown to induce the expression of defense-related genes when expressed in strawberry leaves (Cui et al 2017).…”

Section: Discussionmentioning

confidence: 99%

Disease Resistance Genetics and Genomics in Octoploid Strawberry

Barbey

Lee

Verma

et al. 2019

G3 Genes|Genomes|Genetics

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Octoploid strawberry (Fragaria ×ananassa) is a valuable specialty crop, but profitable production and availability are threatened by many pathogens. Efforts to identify and introgress useful disease resistance genes (R-genes) in breeding programs are complicated by strawberry’s complex octoploid genome. Recently-developed resources in strawberry, including a complete octoploid reference genome and high-resolution octoploid genotyping, enable new analyses in strawberry disease resistance genetics. This study characterizes the complete R-gene collection in the genomes of commercial octoploid strawberry and two diploid ancestral relatives, and introduces several new technological and data resources for strawberry disease resistance research. These include octoploid R-gene transcription profiling, dN/dS analysis, expression quantitative trait loci (eQTL) analysis and RenSeq analysis in cultivars. Octoploid fruit eQTL were identified for 76 putative R-genes. R-genes from the ancestral diploids Fragaria vesca and Fragaria iinumae were compared, revealing differential inheritance and retention of various octoploid R-gene subtypes. The mode and magnitude of natural selection of individual F. ×ananassa R-genes was also determined via dN/dS analysis. R-gene sequencing using enriched libraries (RenSeq) has been used recently for R-gene discovery in many crops, however this technique somewhat relies upon a priori knowledge of desired sequences. An octoploid strawberry capture-probe panel, derived from the results of this study, is validated in a RenSeq experiment and is presented for community use. These results give unprecedented insight into crop disease resistance genetics, and represent an advance toward exploiting variation for strawberry cultivar improvement.

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Section: Discussionmentioning

confidence: 99%

Disease Resistance Genetics and Genomics in Octoploid Strawberry

Barbey

Lee

Verma

et al. 2019

G3 Genes|Genomes|Genetics

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“…Transgenic tobacco plants expressing RPW8.1 and RPW8.2 showed enhanced resistance to powdery mildew . More recently, heterologous expression of RPW8.1 in rice and RPW8.2 in grapevine has also been shown to increase resistance to Magnaporthe oryzae (Li et al, 2018) and powdery mildew (Hu et al, 2018), respectively. Both RPW8.1 and RPW8.2 (noted as RPW8 in later text, unless indicated otherwise) encode a small protein (~20 kDa) with a predicted CC domain.…”

Section: Introductionmentioning

confidence: 99%

An rpw8 quadruple mutant of Arabidopsis Col-0 is partially compromised in bacterial and fungal resistance

Castel

Xiao

et al. 2019

Preprint

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“…ROS are involved in the hypersensitive response (HR) as well as elicitor signal transduction system ( Allen et al, 2004 ; Hu et al, 2018 ; Jones and Dangl, 2006 ). ROS have been associated with the accumulation of plant secondary metabolites and are ultimately required for enhancing plant immunity reactions ( Asai et al, 2008 ; Somasundaram et al, 2018 ; Zhao et al, 2005 ).…”

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confidence: 99%