Ectopic Expression of Cold Responsive LlaCIPK Gene Enhances Cold Stress Tolerance in Nicotiana tabacum

Abstract

Low-temperature stress severely affects the growth, development, and geographical distribution of various crop plants, resulting in significant economic loss to producers. In a quest to identify cold-regulated genes, we constructed a cDNA suppression subtractive library from a high altitude adapted ecotype of Lepidium. We cloned a cold-induced gene LlaCIPK from the subtracted cDNA library which gave homology to Arabidopsis CIPK15 gene. The predicted 3D structure of LlaCIPK protein also showed homology with Arabidopsis CIPK protein. Quantitative real-time PCR analysis in Lepidium seedlings exposed to 6 h of cold stress shows a 3-fold increase in the expression of LlaCIPK transcript. The expression of LlaCIPK was also differentially regulated by ethylene, CaCl2, ABA, and SA treatments. Ethylene and CaCl2 treatments up regulated LlaCIPK expression, whereas ABA and SA treatments down regulated the LlaCIPK expression. Transgenic plants overexpressing LlaCIPK gene under constitutive promoter show an increased level of proline and cell membrane stability. Taken together, our results suggest that the LlaCIPK contributes to the cold-response pathway in Lepidium plants.

MsCIPK, a CBL-interacting Protein Kinase in Medicago sativa L., Confers Salt and Osmotic Stress Tolerance in Transgenic Tobacco

“…CBLs and CIPKs in Arabidopsis revealed that these genes responded to various stresses, including drought, salinity and osmotic stresses 9,14,15 . Similar phenomena were also recently observed on diverse species, such as rice 14,16,17 , maize 18 , wheat 19 , soybean 20 , tobacco 21 , apple 22 , pepper 23 , eggplant 24 , pear 25 and grapewine 26 . However, whether CBL and CIPKs are involved in mediating different stress responses remains largely unknown in alfalfa.…”

Molecular Evolution of Calcium Signaling and Transport in Plant Adaptation to Abiotic Stress

“…Meanwhile, there are seven DEGs actively modulated in all abiotic stress exposures, namely, GLR2.5, CNGC12, OSCA1.3, OSCA2.1, CML38, CIPK15, and CIPK16. Previous studies have demonstrated that the expression of GLR2.5 displays a notabe elevation under salt treatment conditions [165] and that the CIPK15 homologous gene could improve cold stress resistance in Nicotiana tabacum [166]. Screening by forward genetics, AtCIPK16 was identified as a mediator diminishing shoot salt accumulation and enhancing salinity tolerance in Arabidopsis and transgenic barley [167].…”

Genome-Wide Characterization and Analysis of CIPK Gene Family in Two Cultivated Allopolyploid Cotton Species: Sequence Variation, Association with Seed Oil Content, and the Role of GhCIPK6

“…Analysis of cis-elements in genes could provide critical evidence for the gene's function. Many studies reported that CIPK genes play key roles in various stresses [17,22,25]. Here, the putative stress-related and hormone-related cis-elements were scanned in the 1.5 kb upstream of the start codons of 80 GhCIPKs using the PlantCARE database.…”

“…Previous studies reported that CIPK genes could play important roles in plant responses to various stresses [14,22,25,44]. Therefore, we analyzed the expression patterns of the 80 GhCIPKs under various stresses, including cold, hot, salt, and drought stresses, using the published RNA-seq data of TM-1.…”

“…Overexpression of each of the 12 HsCIPKs from barley (Hordeum spontaneum) in rice shows that all but HsCIPK28 enhance the tolerance of roots to multiple heavy metal toxicities, and eight HsCIPKs (except for HsCIPK9, HsCIPK11, HsCIPK14, and HsCIPK24) improve the tolerance to salt, drought, and ABA [24]. Overexpressing the LlaCIPK gene from Lepidium can enhance cold tolerance in tobacco by increasing the level of proline and cell membrane stability [25]. Although the CIPK gene family was reported in G. arboreum and G. raimondii [19], little is known about their detailed information in G. hirsutum and G. barbadense, especially their potential roles in various abiotic stresses and cottonseed oil regulation.…”