Ectopic expression of the human adenine nucleotide translocase, isoform 3 (ANT-3). Characterization of ligand binding properties

A microarray approach identifies ANT, OS-D and takeout-like genes as differentially regulated in alate and apterous morphs of the green peach aphid Myzus persicae (Sulzer)

“…These proteins are embedded in the inner membrane of the mitochondria and function as carriers of important metabolites involved in diverse mitochondrial processes (Pebay-Peyroula and Brandolin, 2004). This carrier comprises up to 10% of all proteins in the inner membrane and imports ADP into the mitochondrial matrix in exchange for synthesized ATP (Kunji, 2004;Carroll et al, 2005). The Mp ANT expression and the higher protein levels (Fig.…”

“…Among the differentially regulated genes that we identified, three genes were further investigated. The first is a new adenine nucleotide translocase (ANT) from M. persicae, that we denoted M. persicae (Mp) ANT, and was previously shown to act as a mitochondrial carrier protein (Carroll et al, 2005). The second gene is also a new M. persicae gene, and is a homolog of the takeout-like/ juvenile hormone binding proteins (TOL/JHBP) (SarovBlat et al, 2000), which we denoted Mp TOL, and was previously reported to be expressed in the antennae of Drosophila, and its expression is related to locomotor activity.…”

Targeting mitochondrial permeability in cancer drug development

“…What constitutes the core structure of the pore complex responsible for the permeability transition phenomenon? Are other ANT isoforms such as ANT3 [48,49] and AAC4 [50], not targeted by Kokoszka et al [35], key components of the pore complex, or are other carrier or shuttle proteins involved? What implications does this have for the numerous small molecules, including anticancer drugs, which affect the MPT and result in mitochondrial membrane permeabilization and subsequent cell death?…”

“…ANT: the , so-called' ANT ligands ADP, ATR and BKA bind to dimeric ANT and inhibit nucleotide translocation across the IMM [68,91,92]. Mitochondria from cells expressing human ANT3 exhibited similar low (ADP) and high (ATR and BKA) affinity binding compared with mitochondria from mammalian cardiac mitochondria [48] which express ANT1 and 2. Another ANT inhibitor, MT-21, caused cytochrome c release without mitochondrial swelling [93] suggesting an MPT-independent mechanism of action similar to ATR.…”