Ectopic over-expression of peroxisomal ascorbate peroxidase (SbpAPX) gene confers salt stress tolerance in transgenic peanut (Arachis hypogaea)

Singh, Narendra Kumar; Mishra, Avinash; Jha, Bhavanath

doi:10.1016/j.gene.2014.06.037

Cited by 86 publications

(41 citation statements)

References 39 publications

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“…This study demonstrates that the ectopic expression of SbSDR1 confers salt and osmotic tolerance at different developmental stages of the plant in pots and also in the field. Many studies have demonstrated that different salt-responsive genes, such as SbNHX1 , SbpAPX1 and SbASR-1 , improved the tolerance of crops such as jatropha, castor and peanut to oxidative stress24252637. The SbpAPX gene confers salinity and drought tolerance to tobacco plants at different developmental stages5.…”

Section: Discussionmentioning

confidence: 99%

“…One extreme halophyte, Salicornia brachiata , which grows profusely on salt marshes, is a suitable candidate with which to study the salt stress-tolerance mechanism19. Several abiotic stress-responsive genes and promoters have been cloned from this halophyte to develop abiotic stress-tolerant transgenic plants202122232425262728. Salicornia also possesses sulphur-rich seed-storage proteins29, unique oligosaccharides30 and metabolites31.…”

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confidence: 99%

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A novel transcription factor-like gene SbSDR1 acts as a molecular switch and confers salt and osmotic endurance to transgenic tobacco

Singh¹,

Mishra²,

Haque³

et al. 2016

Sci Rep

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A salt- and drought-responsive novel gene SbSDR1 is predominantly localised to the nucleus, up-regulated under abiotic stresses and is involved in the regulation of metabolic processes. SbSDR1 showed DNA-binding activity to genomic DNA, microarray analysis revealed the upregulation of host stress-responsive genes and the results suggest that SbSDR1 acts as a transcription factor. Overexpression of SbSDR1 did not affect the growth and yield of transgenic plants in non-stress conditions. Moreover, the overexpression of SbSDR1 stimulates the growth of plants and enhances their physiological status by modulating the physiology and inhibiting the accumulation of reactive oxygen species under salt and osmotic stress. Transgenic plants that overexpressed SbSDR1 had a higher relative water content, membrane integrity and concentration of proline and total soluble sugars, whereas they showed less electrolyte leakage and lipid peroxidation than wild type plants under stress conditions. In field conditions, SbSDR1 plants recovered from stress-induced injuries and could complete their life cycle. This study suggests that SbSDR1 functions as a molecular switch and contributes to salt and osmotic tolerance at different growth stages. Overall, SbSDR1 is a potential candidate to be used for engineering salt and drought tolerance in crops without adverse effects on growth and yield.

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Section: Discussionmentioning

confidence: 99%

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confidence: 99%

A novel transcription factor-like gene SbSDR1 acts as a molecular switch and confers salt and osmotic endurance to transgenic tobacco

Singh¹,

Mishra²,

Haque³

et al. 2016

Sci Rep

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“…Loss of function APX2 mutants also revealed the importance of this isoform in plant growth and development and such mutants were over sensitive to drought as compared to over-expression lines. A mAPX from Salicornia brachiata in over-expression lines provided increased drought tolerance compared to control plants (Zhang et al, 2013; Singh et al, 2014). Faize and colleagues revealed the importance of cAPX in drought stress tolerance in tobacco where a major beneficial effect was on membrane protection (Faize et al, 2011).…”

Section: Expression Of Apxs Under Drought Conditionsmentioning

confidence: 99%

Abiotic Stress Tolerance in Plants: Myriad Roles of Ascorbate Peroxidase

et al. 2017

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One of the most significant manifestations of environmental stress in plants is the increased production of Reactive Oxygen Species (ROS). These ROS, if allowed to accumulate unchecked, can lead to cellular toxicity. A battery of antioxidant molecules is present in plants for keeping ROS levels under check and to maintain the cellular homeostasis under stress. Ascorbate peroxidase (APX) is a key antioxidant enzyme of such scavenging systems. It catalyses the conversion of H2O2 into H2O, employing ascorbate as an electron donor. The expression of APX is differentially regulated in response to environmental stresses and during normal plant growth and development as well. Different isoforms of APX show differential response to environmental stresses, depending upon their sub-cellular localization, and the presence of specific regulatory elements in the upstream regions of the respective genes. The present review delineates role of APX isoforms with respect to different types of abiotic stresses and its importance as a key antioxidant enzyme in maintaining cellular homeostasis.

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“…Some species of Salicornia have shown important biological properties, such as antioxidant, anti-inflammatory, hypoglycemic and cytotoxic activities (Isca, Seca, Pinto, & Silva, 2014). Furthermore, Salicornia is being widely explored for abiotic stress-responsive genes and promoters to develop transgenic plants that can withstand adverse environmental conditions (Chaturvedi, Mishra, Tiwari, & Jha, 2012;Chaturvedi, Patel, Mishra, Tiwari, & Jha, 2014;Jha, Sharma, & Mishra, 2011;Singh, Mishra, & Jha, 2014a, 2014bUdawat, Mishra, & Jha, 2014). No information is available, so far, on the metabolomics of this important succulent halophytic plant.…”

Section: Introductionmentioning

confidence: 99%

Non-targeted metabolomics and scavenging activity of reactive oxygen species reveal the potential of Salicornia brachiata as a functional food

Mishra

Patel

Jha

2015

Journal of Functional Foods

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Ectopic over-expression of peroxisomal ascorbate peroxidase (SbpAPX) gene confers salt stress tolerance in transgenic peanut (Arachis hypogaea)

Cited by 86 publications

References 39 publications

A novel transcription factor-like gene SbSDR1 acts as a molecular switch and confers salt and osmotic endurance to transgenic tobacco

A novel transcription factor-like gene SbSDR1 acts as a molecular switch and confers salt and osmotic endurance to transgenic tobacco

Abiotic Stress Tolerance in Plants: Myriad Roles of Ascorbate Peroxidase

Non-targeted metabolomics and scavenging activity of reactive oxygen species reveal the potential of Salicornia brachiata as a functional food

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