Ectopic Pregnancy Is Associated with High Anandamide Levels and Aberrant Expression of FAAH and CB1 in Fallopian Tubes

Gebeh, Alpha K.; Willets, Jonathon M.; Marczylo, Emma L.; Taylor, Anthony H.; Konje, Justin C.

doi:10.1210/jc.2012-1780

Cited by 47 publications

(47 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Even so, the AEA concentration in human follicular fluid has been reported to rise during oocyte maturation (El-Talatini et al 2009) and this concentration is even higher in the oviduct (Gebeh et al 2012). In humans, on average, one oocyte completes growth each month and is ovulated in response to a mid-cycle surge of LH and in response to this LH surge, the oocyte resumes meiosis (Hunt and Hassold 2008).…”

Section: Discussionmentioning

confidence: 99%

Dynamic of expression and localization of cannabinoid-degrading enzymes FAAH and MGLL in relation to CB1 during meiotic maturation of human oocytes

et al. 2016

View full text Add to dashboard Cite

The endogenous cannabinoid system has been characterized in some female reproductive organs but little is known about the expression and localization pattern of cannabinoid-degrading enzymes in relation to the CB1 cannabinoid receptor in human oocytes. In this study, we focus on the investigation of the presence and differential distribution of fatty acid amide hydrolase (FAAH) and monoglyceride lipase (MGLL) in relation to CB1 during the maturation of human oocytes. We used a total of 290 human oocytes not suitable for in vitro fertilization/intracytoplasmic sperm injection (ICSI): germinal-vesicle (GV) and metaphase-I (MI) stages and metaphase-II (MII) oocytes that had not developed into an embryo after ICSI. Cannabinoid-degrading enzymes and the cannabinoid CB1 receptor were present in human oocytes. Specifically, FAAH was detected in the periphery of the oocyte from the GV to MI stage and co-localized with CB1. Later, by the MII stage, FAAH was spread within the oocyte, whereas MGLL immunostaining was homogeneous across the oocyte at all stages of maturation and only overlapped with CB1 at the GV stage. This coordinated redistribution of cannabinoid system proteins suggests a role for this system in the maturation of the female gamete.

show abstract

Section: Discussionmentioning

confidence: 99%

Dynamic of expression and localization of cannabinoid-degrading enzymes FAAH and MGLL in relation to CB1 during meiotic maturation of human oocytes

et al. 2016

View full text Add to dashboard Cite

show abstract

“…Contribution from the epidydimis, prostate, and seminal vesicles is possible. In addition, cannabinoid receptors and endocannabinoid synthetic and degradation enzymes have been localized in fallopian tubal epithelium, which indicates exposure of spermatozoa to the endocannabinoids in the female genital tract (45)(46)(47)(48).…”

Section: Discussionmentioning

confidence: 99%

Relationship between seminal plasma levels of anandamide congeners palmitoylethanolamide and oleoylethanolamide and semen quality

Amoako

Marczylo²,

Elson

et al. 2014

Fertility and Sterility

Self Cite

View full text Add to dashboard Cite

“…FAAH activity in peripheral blood cell membranes is significantly lower in women with ectopic pregnancies than in those with normal pregnancies (Gebeh et al 2013). Similarly, the expression of FAAH mRNA is also significantly lower in the fallopian tubes of women with ectopic pregnancies than in those of women in the luteal or follicular phase of their menstrual cycle (Gebeh et al 2012). Furthermore, plasma anandamide levels are significantly higher in women with ectopic pregnancies than in those with normal pregnancies (Gebeh et al 2013).…”

Section: Endocannabinoids In Ectopic Pregnanciesmentioning

confidence: 95%

“…FAAH, NAPE-PLD, and CB1 and CB2 receptors can be strongly detected in the luminal border of the fallopian tube epithelium (Gebeh et al 2012). FAAH activity in peripheral blood cell membranes is significantly lower in women with ectopic pregnancies than in those with normal pregnancies (Gebeh et al 2013).…”

Section: Endocannabinoids In Ectopic Pregnanciesmentioning

confidence: 96%

The role of endocannabinoids in pregnancy

et al. 2013

View full text Add to dashboard Cite

Endocannabinoids are a family of lipid signalling molecules. As with prostaglandins (PGs), endocannabinoids are derived from polyunsaturated fatty acids and affect cell function via receptor-mediated mechanisms. They also bind to PG receptors, although at a lower affinity. The endocannabinoid network is regulated in pregnancy from embryo development to labour onset. Even small changes in endocannabinoid exposure can retard embryo development and affect implantation success. There is now compelling evidence that aberrant expression of factors involved in the endocannabinoid pathway in the placenta and circulating lymphocytes results in spontaneous miscarriage and poor pregnancy outcomes. It is likely that competition between endocannabinoids, PGs and other similar lipids ultimately determines how phospholipid/fatty acid substrates are metabolised and, thus, the balance between the uterotonic and tocolytic activities. We, therefore, hypothesise that endocannabinoid profiles may be used as a biomarker to predict and/or identify spontaneous labour onset.Reproduction (2013) 146 R101-R109

show abstract

Ectopic Pregnancy Is Associated with High Anandamide Levels and Aberrant Expression of FAAH and CB1 in Fallopian Tubes

Abstract: High anandamide levels and reduced expression of CB1 and FAAH may play a role in ectopic implantation.

Cited by 47 publications

References 36 publications

Dynamic of expression and localization of cannabinoid-degrading enzymes FAAH and MGLL in relation to CB1 during meiotic maturation of human oocytes

Dynamic of expression and localization of cannabinoid-degrading enzymes FAAH and MGLL in relation to CB1 during meiotic maturation of human oocytes

Relationship between seminal plasma levels of anandamide congeners palmitoylethanolamide and oleoylethanolamide and semen quality

The role of endocannabinoids in pregnancy

Contact Info

Product

Resources

About