ED-XRF spectrometric analysis of comparative elemental composition of in vivo and in vitro roots of Andrographis paniculata (Burm.f.) Wall. ex Nees—a multi-medicinal herb

Andrographis paniculata(Burm. f.) Wall. ex Nees: A Review of Ethnobotany, Phytochemistry, and Pharmacology

“…Arifullah et al [ 141 ] isolated andrographolide and echiodinin from in vitro leaf callus of AP. In vitro root culture system can be exploited as a renewable source of minerals essential for designing effective drugs [ 167 ]. Other bioactive compounds can also be extracted via tissue culture.…”

Regeneration of Andrographis paniculata Nees: Analysis of genetic fidelity and andrographolide content in micropropagated plants

“…Except for the preliminary study of micropropagation by Martin (2004), Purkayastha et al (2008), Handique (2010, 2011) and Basu and Yogananth (2011), no comprehensive in vitro studies have been reported on A. paniculata. Behera et al (2010) reported similar composition of trace elements among in vivo and in vitro regenerated roots of A. paniculata. Here we describe an efficient protocol for direct plant regeneration from nodal explants of A. paniculata along with the randomly amplified polymorphic deoxyribonucleic acid (DNA) (RAPD) analysis to determine the genetic fidelity of the regenerated plants and analysis of andrographolide content in regenerated plants.…”

Atomic spectrometry update-X-ray fluorescence spectrometry

“…Bennal et al 42 determined the K-L vacancy transfer probabilities for selected elements in the atomic range 42 # Z # 82 by using gamma 57 Co source excitation, also reporting good agreement with literature data. Mass absorption coefficients of selected semiconductor materials and biological matrices were determined experimentally at different X-ray energies by Ekinci et al 43 Cengiz et al 44 determined L-shell X-ray production cross-sections, fluorescence yields, and vacancy transfer probabilities for selected heavy elements by using 241 Am excitation. In another report by Cengiz and co-workers 45 K-shell X-ray production cross-sections, fluorescence yields, K-L shell vacancy transfer probabilities, and the natural line widths of selected heavy elements from the range 73 # Z # 81 were measured.…”