ED27 Trophoblast-like Cells Isolated from First-trimester Chorionic Villi are Genetically Identical to HeLa Cells Yet Exhibit a Distinct Phenotype

Kniss, Douglas A.; Xie, Yizhen; Li, Y.; Kumar, Sanjay; Linton, E.A.; Cohen, Paul A.; Fan-Havard, Patty; Redman, Chanelle; Sargent, Ian L.

doi:10.1053/plac.2001.0749

Cited by 41 publications

(23 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The nature of the deposits collected in OM-treated HeLa cultures showed a calcium phosphate component; however, the mineral did not seem to be as crystalline or extracellular matrix-bound as observed in MSC cultures. In addition, HeLa cells were shown to be strongly positive for ALP activity, in line with reports that they express several ALP isoforms [Boylan et al, 1996;Kniss et al, 2002]. This could explain the substantial mineral content observed under OM conditions, as high ALP activity has been reported to contribute to unspecific mineral deposition in other systems [Russell et al, 1986;Khouja et al, 1990;Hui et al, 1997].…”

Section: Discussionsupporting

confidence: 77%

Differences in the Pattern and Regulation of Mineral Deposition in Human Cell Lines of Osteogenic and Non-Osteogenic Origin

Rashidi

Strohbuecker²,

Jackson³

et al. 2011

Cells Tissues Organs

View full text Add to dashboard Cite

Bone marrow-derived mesenchymal stem cells (MSCs) are widely used as a cellular model of bone formation, and can mineralize in vitro in response to osteogenic medium (OM). It is unclear, however, whether this property is specific to cells of mesenchymal origin. We analysed the OM response in 3 non-osteogenic lines, HEK293, HeLa and NTera, compared to MSCs. Whereas HEK293 cells failed to respond to OM conditions, the 2 carcinoma-derived lines NTera and HeLa deposited a calcium phosphate mineral comparable to that present in MSC cultures. However, unlike MSCs, HeLa and NTera cultures did so in the absence of dexamethasone. This discrepancy was confirmed, as bone morphogenetic protein inhibition obliterated the OM response in MSCs but not in HeLa or NTera, indicating that these 2 models can deposit mineral through a mechanism independent of established dexamethasone or bone morphogenetic protein signalling.

show abstract

Section: Discussionsupporting

confidence: 77%

Differences in the Pattern and Regulation of Mineral Deposition in Human Cell Lines of Osteogenic and Non-Osteogenic Origin

Rashidi

Strohbuecker²,

Jackson³

et al. 2011

Cells Tissues Organs

View full text Add to dashboard Cite

show abstract

“…We showed H1R and H2R expressions in primary cultured human amniotic cells in this report. We also showed the expressions in WISH cells; however, WISH contains the possibility of contamination of HeLa cells and we did not confirm the existence of HeLa markers in the cells we used [32] .…”

Section: Discussionmentioning

confidence: 64%

Histamine Stimulates Interleukin-6 Production through Histamine H1 Receptors in Human Amnion Cells

Morimitsu

Sato

Kotani

et al. 2009

Gynecol Obstet Invest

View full text Add to dashboard Cite

Background/Aims: Previous studies have stated that maternal allergic diseases are associated with increased risk of preterm labor/delivery, but the underlying mechanisms remain unclear. This study tested the hypothesis that histamine induces interleukin (IL)-6 production in amnion cells. Methods: Using cultured human amnion cells, we examined expression of histamine receptors and effects of histamine on IL-6 production. Results: Reverse transcription-polymerase chain reaction and Western blotting revealed expression of histamine H1 receptor (H1R) and H2 receptor (H2R) in human amnion. Histamine stimulation significantly increased concentrations of IL-6 in conditioned medium, as did tumor necrosis factor-α and IL-1β in positive controls. In addition, the H1R antagonist olopatadine significantly blocked histamine-induced production of IL-6, whereas the H2R antagonist ranitidine did not. Conclusion: Histamine appears to induce IL-6 production through H1R in human amnion cells.

show abstract

“…The STR analysis, also known as DNA fingerprinting, the gold standard for cell authentication, demonstrated identity with HeLa cells and WISH cells. The WISH cells, initially isolated at the Wistar Institute by Hayflick 28 from primary amnion epithelium, are known to be contaminated with HeLa cells 29 and many in the parturition research community have used them. [6][7][8][30][31][32] Importantly, at the time when the HES cell line was isolated in our laboratory, we did not use HeLa cells.…”

Section: Discussionmentioning

confidence: 99%

Discovery of HeLa Cell Contamination in HES Cells: Call for Cell Line Authentication in Reproductive Biology Research

Kniss

Summerfield

2014

Reprod. Sci.

View full text Add to dashboard Cite

Continuous cell lines are used frequently in reproductive biology research to study problems in early pregnancy events and parturition. It has been recognized for 50 years that many mammalian cell lines contain inter-or intraspecies contaminations with other cells. However, most investigators do not routinely test their culture systems for cross-contamination. The most frequent contributor to cross-contamination of cell lines is the HeLa cell isolated from an aggressive cervical adenocarcinoma. We report on the discovery of HeLa cell contamination of the human endometrial epithelial cell line HES isolated in our laboratory. Short tandem repeat analysis of 9 unique genetic loci demonstrated molecular identity between HES and HeLa cells. In addition, we verified that WISH cells, isolated originally from human amnion epithelium, were also contaminated with HeLa cells. Inasmuch as our laboratory did not culture HeLa cells at the time of HES cell derivations, the source of contamination was the WISH cell line. These data highlight the need for continued diligence in authenticating cell lines used in reproductive biology research.

show abstract

ED27 Trophoblast-like Cells Isolated from First-trimester Chorionic Villi are Genetically Identical to HeLa Cells Yet Exhibit a Distinct Phenotype

Cited by 41 publications

References 27 publications

Differences in the Pattern and Regulation of Mineral Deposition in Human Cell Lines of Osteogenic and Non-Osteogenic Origin

Differences in the Pattern and Regulation of Mineral Deposition in Human Cell Lines of Osteogenic and Non-Osteogenic Origin

Histamine Stimulates Interleukin-6 Production through Histamine H1 Receptors in Human Amnion Cells

Discovery of HeLa Cell Contamination in HES Cells: Call for Cell Line Authentication in Reproductive Biology Research

Contact Info

Product

Resources

About