EDC cross-linking improves skin substitute strength and stability

Crosslinking and Mechanical Properties Significantly Influence Cell Attachment, Proliferation, and Migration Within Collagen Glycosaminoglycan Scaffolds

“…1). 14,17 This means that EDAC is limited to crosslinking collagen molecules that are directly adjacent to each other (1 nm), whereas GTA can crosslink molecules that are separated by a distance. 18 The nature of this chemical crosslinking involves the use of reagents that have the potential to cause cell death if they remain within the scaffold.…”

Local and regional mechanical characterisation of a collagen-glycosaminoglycan scaffold using high-resolution finite element analysis

“…However, on a scaffoldlevel, previous investigations have demonstrated that scaffold volume fraction can influence cell infiltration: the larger the pore size (lower volume fraction) the more the cells disperse throughout the scaffold due the increased fluid movement [Byrne et al, 2008;Tierney et al, 2008]; though interestingly without fluid influences, and when contact guidance is measured (cell motility along scaffold struts), cells migrate less in larger pores (151µm to 96µm) . Similarly, biosynthesis and degradation rates have also been shown to vary according to scaffold properties, such as cross-link densities and stiffness, which in part is related to relative density [Chevallay et al, 2000;Lee et al, 2001;Petite et al, 1994;Powell et al, 2006;Vickers et al, 2006;Weadcock et al, 1996]. Thus, it is likely the regional variances do impact on cell activity and tissue growth.…”

Physiochemical properties and resorption progress of porcine skin-derived collagen membranes: <i>In vitro</i> and <i>in vivo</i> analysis

“…DHT cross-linking technique in collagen membranes could promote favorable mechanical properties, more or less, due to the formation of ester and amide bonds 22) . EDC is regarded as a zero-length cross-linker, which does not retain reagents harmful to the membrane itself 23) . The present study was designed to evaluate two cross-linked collagen membranes (DHT and DHT/EDC) derived from PD for GBR/GTR procedures, and compare their features with those of a membrane without cross-linking (BG) in vitro and in vivo.…”

“…Membrane porosity and 3D architecture are considered to be pivotal for osteoblast infiltration and proliferation in the early phase of GBR/GTR 10) . Powell and Boyce 23) proposed that appropriate porosity should be >55%. Enea et al 24) reported that an interconnected 3D architecture was important for functional performance.…”