EDC-Mediated Oligonucleotide Immobilization on a Long Period Grating Optical Biosensor

Chen, Xianfeng; Liu, Chen; Hughes, Marcus D.; Nagel, David A.; Hine, Anna V.; Zhang, Lin

doi:10.4172/2155-6210.1000173

Cited by 26 publications

(20 citation statements)

References 33 publications

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“…The DNA probe was covalently immobilized on the grating surface and was subsequently hybridized with the complementary target DNA sequence. Recently, the same authors presented an improvement in the immobilization of the DNA probe, one that allowed a detectable oligonucleotide concentration of 4 nM [78]. Other researchers have explored DNA hybridization by using LPG sensors [79,80,81] and LPG in MOF [37].…”

Section: Biosensorsmentioning

confidence: 99%

Towards a Uniform Metrological Assessment of Grating-Based Optical Fiber Sensors: From Refractometers to Biosensors

et al. 2017

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A metrological assessment of grating-based optical fiber sensors is proposed with the aim of providing an objective evaluation of the performance of this sensor category. Attention was focused on the most common parameters, used to describe the performance of both optical refractometers and biosensors, which encompassed sensitivity, with a distinction between volume or bulk sensitivity and surface sensitivity, resolution, response time, limit of detection, specificity (or selectivity), reusability (or regenerability) and some other parameters of generic interest, such as measurement uncertainty, accuracy, precision, stability, drift, repeatability and reproducibility. Clearly, the concepts discussed here can also be applied to any resonance-based sensor, thus providing the basis for an easier and direct performance comparison of a great number of sensors published in the literature up to now. In addition, common mistakes present in the literature made for the evaluation of sensor performance are highlighted, and lastly a uniform performance assessment is discussed and provided. Finally, some design strategies will be proposed to develop a grating-based optical fiber sensing scheme with improved performance.

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Section: Biosensorsmentioning

confidence: 99%

Towards a Uniform Metrological Assessment of Grating-Based Optical Fiber Sensors: From Refractometers to Biosensors

et al. 2017

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“…This has also contributed to the increase in interest in label-free sensing using LPGs, especially at the PMTP where there is high RI sensitivity, rapid response and adaptability by choice of overlay [18,72,78,79]. LPGs at PMTP have been used for real time monitoring of phage-bacteria interactions [80,81] where a 1.3 nm wavelength shift was detected as bacteria binding Optical Fibre Long-Period Grating Sensors Operating at and around the Phase Matching… DOI: http://dx.doi.org /10.5772/intechopen.81179 occurred [80], and target-probe DNA hybridisation [82,83]. The well-known properties of the streptavidin-biotin interaction, as well as its use for studying biological processes, have encouraged its use as a means of understanding the characteristics of thin film PMTP sensors [21,55].…”

Section: Chemical and Gas Sensingmentioning

confidence: 99%

Optical Fibre Long-Period Grating Sensors Operating at and around the Phase Matching Turning Point

Wong¹,

Juan²,

Ibsen³

et al. 2019

Applications of Optical Fibers for Sensing

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Optical fibres have been exploited as sensors for many years and they provide a versatile platform with a small form factor. Long-period gratings (LPGs) operating at and around the phase matching turning point (PMTP) possess some of the highest sensitivities to external perturbations in the family of LPG-based sensor devices. This type of optical fibre grating has been demonstrated as a sensor for use in a wide range of applications. In this review chapter, an overview of PMTP LPGs is presented and the key developments, findings and applications are highlighted. The fabrication considerations and sensor limitations are also discussed.

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“…Different devices have been proposed for this purpose. Among the evanescent-field-based devices we can find long period gratings (LPGs), tilted fiber Bragg gratings (TFBGs) or BIO-Bragg gratings stamped in tapered fibers [7][8][9][10]. Also, surface modes have been exploited for biosensing: optical microfibers can be metallic coated to support surface plasmon resonances [11,12] and whispering gallery modes in microspheres and microbottles have been demonstrated to show really low detection limits [13,14].…”

Section: Introductionmentioning

confidence: 99%

Resonant Couplings in U-Shaped Fibers for Biosensing

Londero¹,

Delgado-Pinar²,

Cuadrado-Laborde

et al. 2023

J. Lightwave Technol.

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U-shaped tight curvatures in optical fibers lead to resonant couplings between the fundamental and higher order modes that are sensible to different parameters, such as strain or temperature, for example. The optical response of the sensor consists on the shift of the resonant wavelength of the coupling. In the case of singlemode fibers, the coupling involves a so-called "cladding mode" and, due to its evanescent field, the curved region will be sensible to changes in the external medium, as well. In this paper, we present the fabrication and characterization of a robust, easy-to-make, U-shaped fiber sensor based on singlemode telecom fiber and its application for biosensing. The resonant nature of the sensing mechanism presents the advantage of large dynamic ranges for RI variations without the ambiguity of other techniques such as interferometry. We studied the performance of the U-shaped fiber sensor for different bending radii, to optimize its sensitivity and detection limit at 1550 nm operation wavelength, as well as the effect of temperature on its response. The shift of the resonant wavelength was measured in detail as a function of the external RI within the range [1.33-1,37]; the detection limit was established in (3.71±0.03)×10 -5 RIU. Furthermore, the device was successfully tested as a proof of concept biosensor, using a system model antigen-antibody (BSA-aBSA.)

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EDC-Mediated Oligonucleotide Immobilization on a Long Period Grating Optical Biosensor

Cited by 26 publications

References 33 publications

Towards a Uniform Metrological Assessment of Grating-Based Optical Fiber Sensors: From Refractometers to Biosensors

Towards a Uniform Metrological Assessment of Grating-Based Optical Fiber Sensors: From Refractometers to Biosensors

Optical Fibre Long-Period Grating Sensors Operating at and around the Phase Matching Turning Point

Resonant Couplings in U-Shaped Fibers for Biosensing

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