Editorial: Biofluid Extracellular Vesicles and Their Involvement in Animal Reproductive Physiology

Saadeldin, Islam M.; Gad, Ahmed; Mermillod, Pascal

doi:10.3389/fvets.2021.747138

Cited by 3 publications

(2 citation statements)

References 7 publications

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“…Amongst other things, oocytes prevent polyspermy by producing CGs that contain enzymes to harden the ZP and prevent subsequent spermatozoa fusion. Recently, EVs have emerged as an important component of several biological fluids, serving a crucial role in communication among different reproductive cells ( 35 ). The present study demonstrated that OEC-EVs significantly increased CG content ( Fig.…”

Section: Discussionmentioning

confidence: 99%

“…Fallopian tube-secreted EVs contribute to improving the developmental competence and the fertilization of oocytes by reducing the ROS levels, eliciting anti-apoptotic effects, improving lipid metabolism, and increasing the expression of reprogramming-related genes ( 11 , 24 ). Many studies have presented isolation, characterization, and microRNA analyses of oviduct EVs in various species ( 12 , 25 , 35 , 36 , 51 ). Oviduct EV characterization identified proteins with critical roles in the gamete/embryo-oviduct interactions, such as OVGP1, heat shock protein (HSP) 90, HSPA8, HSP70, gelsolin and ezrin ( 22 ).…”

Section: Discussionmentioning

confidence: 99%

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Oviduct epithelial cell‑derived extracellular vesicles promote the developmental competence of IVF porcine embryos

et al. 2023

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Assisted reproductive technology has increased the efficiency of animal reproduction. However, polyspermy is a significant limitation of porcine in vitro fertilization (IVF). Therefore, reducing the polyspermy rate and improving monospermic embryos is crucial. Recent studies have reported that oviductal fluid, along with its contents of extracellular vesicles (EVs), enhanced the fertilization process and supported embryo development. Consequently, the present study investigated the effects of porcine oviduct epithelial cells (OEC-EVs) on sperm-oocyte interactions during porcine IVF and evaluated in vitro embryo developmental competence outcomes. During IVF embryo development, the cleavage rate was significantly higher in the group treated with 50 ng/ml OEC-EVs compared with the control group (67.6±2.5 vs. 57.3±1.9; P<0.05). Furthermore, the OEC-EV group had significantly more embryos (16.4±1.2 vs. 10.2±0.8; P<0.05), and the polyspermy rate significantly decreased (32.9±2.5 vs. 43.8±3.1; P<0.05) compared with that of the control group. Additionally, the fluorescence intensities of cortical granules (3.56±0.47 vs. 2.15±0.24; P<0.05) and active mitochondria (8.14±0.34 vs. 5.96±0.38; P<0.05) were significantly higher in the OEC-EV group compared with those in the control group. In conclusion, OEC-EV adsorption and penetration crosstalk between sperm and oocytes was observed. OEC-EV treatment was demonstrated to significantly improve the concentration and distribution of cortical granules in oocytes. Furthermore, OEC-EVs also increased oocyte mitochondrial activity, reduced polyspermy and increased the IVF success rate.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Oviduct epithelial cell‑derived extracellular vesicles promote the developmental competence of IVF porcine embryos

et al. 2023

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Oviduct epithelial cells‐derived extracellular vesicles improve preimplantation developmental competence of in vitro produced porcine parthenogenetic and cloned embryos

Fang

Tanga

Bang

et al. 2021

Molecular Reproduction Devel

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Extracellular vesicles (EVs) carry bioactive cargoes involved in the early preimplantation development. This study investigated the effects of EVs obtained from an oviductal epithelial cell (OEC) conditioned medium on the developmental competence of in parthenogenetic activation (PA) and somatic cell nuclear transfer (SCNT) porcine embryos. The OEC‐EV‐treated group showed significant increases in blastocyst formation and hatching rates compared to the control group (40.8% ± 2.2% and 20.1% ± 2.1% vs. 24.9% ± 2.0% and 5.3% ± 1.1%; p < 0.05), respectively. The 7 day OEC‐EVs treatment group significantly increased blastocyst formation rate than the 3 day and 0 day‐groups (45.0 ± 0.8 vs. 33.0 ± 0.7 and 26.7 ± 0.5; p < 0.05), respectively. SCNT revealed that the OEC‐EV increased blastocyst formation rate compared to that of oviductal fluid EVs (OF‐EVs) (35.4% ± 1.4% vs. 29.3% ± 1.3%; p < 0.05). Reactive oxygen species levels, apoptosis, and blastocyst lipid content were significantly decreased in the OEC‐EVs group compared with the control group. OEC‐EV group showed a significantly decreased BAX and increased BCL2, SOD1, POU5F1, SOX2, NANOG, GATA6, PNPLA2, LIPE, and MGLL gene expression than the control group (p < 0.05). In conclusion, OEC‐EVs supplementation in embryo culture media improved the quality of porcine embryos, potentially helping porcine‐cloned embryonic development possibly through transfer of messenger RNA and proteins to the early embryos.

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Potential roles of extracellular vesicles as a noninvasive tool for prenatal genetic diagnosis

Saadeldin¹,

Ehab²,

Swelum³

2023

F&S Science

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Editorial: Biofluid Extracellular Vesicles and Their Involvement in Animal Reproductive Physiology

Cited by 3 publications

References 7 publications

Oviduct epithelial cell‑derived extracellular vesicles promote the developmental competence of IVF porcine embryos

Oviduct epithelial cell‑derived extracellular vesicles promote the developmental competence of IVF porcine embryos

Oviduct epithelial cells‐derived extracellular vesicles improve preimplantation developmental competence of in vitro produced porcine parthenogenetic and cloned embryos

Potential roles of extracellular vesicles as a noninvasive tool for prenatal genetic diagnosis

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