Editorial: Bioprocess‐oriented plant design – turning basic research into practical applications

Bley, Thomas

doi:10.1002/elsc.201190024

Cited by 2 publications

(1 citation statement)

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“…Our previous investigations [21] showed that the temporary immersion is a prospective technology for galanthamine production by L. aestivum L. shoots; however, there are few but very important limitations: large-scale temporary immersion apparatus is not available; the labor cost during the exploitation of such cultivation is too high. It is well known that the improvement of existed bioreactor designs or development of new constructions is an essential step in development of effective biotechnological processes for secondary metabolite production [30]. Because of these circumstances, we estimated the potential of bubblecolumn bioreactor with regard to galanthamine production.…”

Section: Discussionmentioning

confidence: 99%

Galanthamine production by Leucojum aestivumL. shoot culture in a modified bubble column bioreactor with internal sections

Georgiev

Ivanov

Berkov

et al. 2012

Engineering in Life Sciences

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Shoot culture of summer snowflake (Leucojum aestivum L.) was successfully cultivated in an advanced modified glass‐column bioreactor with internal sections for production of Amaryllidaceae alkaloids. The highest amounts of dry biomass (20.8 g/L) and galanthamine (1.7 mg/L) were achieved when shoots were cultured at 22°C and 18 L/(L·h) flow rate of inlet air. At these conditions, the L. aestivum shoot culture possessed mixotrophic‐type nutrition, synthesizing the highest amounts of chlorophyll (0.24 mg/g DW (dry weight) chlorophyll A and 0.13 mg/g DW chlorophyll B). The alkaloids extract of shoot biomass showed high acetylcholinesterase inhibitory activity (IC50 = 4.6 mg). The gas chromatography–mass spectrometry (GC/MS) profiling of biosynthesized alkaloids revealed that galanthamine and related compounds were presented in higher extracellular proportions while lycorine and hemanthamine‐type compounds had higher intracellular proportions. The developed modified bubble‐column bioreactor with internal sections provided conditions ensuring the growth and galanthamine production by L. aestivum shoot culture.

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Section: Discussionmentioning

confidence: 99%