Editorial for Special Issue: The Chorioallantoic Membrane (CAM) Model—Traditional and State-of-the Art Applications: The 1st International CAM Conference

Schneider‐Stock, Regine; Flügen, Georg

doi:10.3390/cancers15030772

Cited by 5 publications

(5 citation statements)

References 14 publications

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“…Although the chick embryo CAM model has some advantages as an experimental model, it is rarely used for testing polypropylene mesh (23) or collagen-based scaffolds (24,25). Furthermore, the evaluation of the CAM model through the utilization of artificial intelligence-driven techniques has been documented in only ten publications at present (26), with even fewer studies focusing on the implementation of polypropylene mesh and collagen scaffold implants (27)(28)(29).…”

Section: Discussionmentioning

confidence: 99%

“…A limited number of articles have reported on the evaluation of the CAM model using artificial intelligencebased techniques at present (26,27,29), with none specifically focusing on polypropylene polymers. Only a limited number of studies have documented the evaluation of collagen scaffold implants through the use of automated analysis software dedicated to the assessment of CAM derived stereomicroscopic images.…”

Section: Discussionmentioning

confidence: 99%

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Artificial Intelligence (AI) Based Analysis ofIn VivoPolymers and Collagen Scaffolds Inducing Vascularization

SALVANTE,

POPOIU,

BARB

et al. 2024

In Vivo

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Background/Aim: Biomaterials are essential in modern medicine, both for patients and research. Their ability to acquire and maintain functional vascularization is currently debated. The aim of this study was to evaluate the vascularization induced by two collagen-based scaffolds (with 2D and 3D structures) and one non-collagen scaffold implanted on the chick embryo chorioallantoic membrane (CAM). Materials and Methods: Classical stereomicroscopic image vascular assessment was enhanced with the IKOSA software by using two applications: the CAM assay and the Network Formation Assay, evaluating the vessel branching potential, vascular area, as well as tube length and thickness. Results: Both collagen-based scaffolds induced noninflammatory angiogenesis, but the non-collagen scaffold induced a massive inflammation followed by inflammatoryrelated angiogenesis. Vessels branching points/Region of Interest (Px^2) and Vessel branching points/Vessel total area (Px^2), increased exponentially until day 5 of the experiment certifying a sustained and continuous angiogenic process induced by 3D collagen scaffolds. Conclusion: Collagen-based scaffolds may be more suitable for neovascularization compared to non-collagen scaffolds. The present study demonstrates the potential of the CAM model in combination with AI-based software for the evaluation of vascularization in biomaterials. This approach could help to reduce and replace animal experimentation in the pre-screening of biomaterials.Biomaterials assume a pivotal role in the design and development of an extensive array of biomedical products and devices, encompassing, among others, prosthetic heart valves, hip joint replacements, dental implants, and ocular contact lenses. Of particular note is a substantial subset of 620

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Artificial Intelligence (AI) Based Analysis ofIn VivoPolymers and Collagen Scaffolds Inducing Vascularization

SALVANTE,

POPOIU,

BARB

et al. 2024

In Vivo

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“…The CAM model has been widely used in the past to study the hallmarks of cancer, such as angiogenesis, proliferation, and tumor invasion, as well as to analyze the conditions underlying cancer therapies [ 100 ]. The process of developing a vascular network that supplies nutrients and oxygen to tumor cells has obviously been the subject of multiple studies, because growing tumors take advantage of the host’s physiological angiogenesis and promote its exuberant development to secure adequate oxygen and nutrient supply, to dispose of waste products, and to facilitate the dissemination of tumor cells to other districts [ 101 ].…”

Section: The Application Of Cam Up To Nowmentioning

confidence: 99%

“…Along with this feature, the intrinsic aggressiveness of various tumor forms was found to have more explanatory elements in diagnostic and therapeutic phases. In this context, by supplying the chicken embryo’s circulatory system with blood and nutrients, CAM provides an ideal system by recreating the physiological microenvironment for the cell–cell and cell–matrix interaction studies that occur during the metastatic cascade [ 100 ]. After the injection of tumor cells into the circulatory system of the chicken embryo, metastatic potential can be assessed by tracking the mRNA levels of metastasizing cancer cells in chick embryos.…”

Section: The Application Of Cam Up To Nowmentioning

confidence: 99%

CAM Model: Intriguing Natural Bioreactor for Sustainable Research and Reliable/Versatile Testing

Palumbo,

Sisi,

Checchi

2023

Biology

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We are witnessing the revival of the CAM model, which has already used been in the past by several researchers studying angiogenesis and anti-cancer drugs and now offers a refined model to fill, in the translational meaning, the gap between in vitro and in vivo studies. It can be used for a wide range of purposes, from testing cytotoxicity, pharmacokinetics, tumorigenesis, and invasion to the action mechanisms of molecules and validation of new materials from tissue engineering research. The CAM model is easy to use, with a fast outcome, and makes experimental research more sustainable since it allows us to replace, reduce, and refine pre-clinical experimentation (“3Rs” rules). This review aims to highlight some unique potential that the CAM-assay presents; in particular, the authors intend to use the CAM model in the future to verify, in a microenvironment comparable to in vivo conditions, albeit simplified, the angiogenic ability of functionalized 3D constructs to be used in regenerative medicine strategies in the recovery of skeletal injuries of critical size (CSD) that do not repair spontaneously. For this purpose, organotypic cultures will be planned on several CAMs set up in temporal sequences, and a sort of organ model for assessing CSD will be utilized in the CAM bioreactor rather than in vivo.

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“…In-ovo methods comprise experiments using chorio-allantois membrane (CAM), vaccine research and production, toxicity studies as well as cardiovascular research, just to name a few. The largest research area is covered by CAM assays, because this highly vascularized membrane enables basic research regarding cancer (tumor cell growth [9], xenografts [10], epithelialmesenchymal transition [11], circulating tumor cells [12]), angiogenesis (pro-and antiangiogenetic drugs) [13,14], wound healing, stem cells [15] and even serves as training platform for surgical procedures [16]. As these models have been shown to provide essential information about cell interactions, cancer and drug effects [17], they are suitable to answer important questions of basic research [18,19].…”

Section: Introductionmentioning

confidence: 99%

Different narcotic gases and concentrations for immobilization of ostrich embryos for in-ovo imaging

Perkas,

Schmidt,

Kuehnel

et al. 2024

Exp. Biol. Med.

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In-ovo imaging using avian eggs has been described as a potential alternative to animal testing using rodents. However, imaging studies are hampered by embryonal motion producing artifacts. This study aims at systematically comparing isoflurane, desflurane and sevoflurane in three different concentrations in ostrich embryos. Biomagnetic signals of ostrich embryos were recorded analyzing cardiac action and motion. Ten groups comprising eight ostrich embryos each were investigated: Control, isoflurane (2%, 4%, and 6%), desflurane (6%, 12%, and 18%) and sevoflurane (3%, 5%, and 8%). Each ostrich egg was exposed to the same narcotic gas and concentration on development day (DD) 31 and 34. Narcotic gas exposure was upheld for 90 min and embryos were monitored for additional 75 min. Toxicity was evaluated by verifying embryo viability 24 h after the experiments. Initial heart rate of mean 148 beats/min (DD 31) and 136 beats/min (DD 34) decreased over time by 44–48 beats/minute. No significant differences were observed between groups. All narcotic gases led to distinct movement reduction after mean 8 min. Embryos exposed to desflurane 6% showed residual movements. Isoflurane 6% and sevoflurane 8% produced motion-free time intervals of mean 70 min after discontinuation of narcotic gas exposure. Only one embryo death occurred after narcotic gas exposure with desflurane 6%. This study shows that isoflurane, desflurane and sevoflurane are suitable for ostrich embryo immobilization, which is a prerequisite for motion-artifact free imaging. Application of isoflurane 6% and sevoflurane 8% is a) safe as no embryonal deaths occurred after exposure and b) effective as immobilization was observed for approx. 70 min after the end of narcotic gas exposure. These results should be interpreted with caution regarding transferability to other avian species as differences in embryo size and incubation duration exist.

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Editorial for Special Issue: The Chorioallantoic Membrane (CAM) Model—Traditional and State-of-the Art Applications: The 1st International CAM Conference

Abstract: In 1959, Russell and Burch published the “Principles of Humane Experimental Technique” [...]

Cited by 5 publications

References 14 publications

Artificial Intelligence (AI) Based Analysis ofIn VivoPolymers and Collagen Scaffolds Inducing Vascularization

Artificial Intelligence (AI) Based Analysis ofIn VivoPolymers and Collagen Scaffolds Inducing Vascularization

CAM Model: Intriguing Natural Bioreactor for Sustainable Research and Reliable/Versatile Testing

Different narcotic gases and concentrations for immobilization of ostrich embryos for in-ovo imaging

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