Groundwater level plays an important role in triggering landslides. In this paper, Distinct Element Method is used to investigate the impact of groundwater table fluctuation on the stability of jointed rock slopes. For this purpose, 110 cases including different number of joint sets, joint friction angles, joint spacings, and joint angles are considered and the influence of changing groundwater level on the stability of a jointed rock slope is investigated through a series of parametric studies. This study shows that the factor of safety for slopes can decrease significantly with increasing the groundwater level, and the impact is more significant on slopes with steeper joints. Furthermore, as the spacing of the joints decreases, the impact decreases. However, as the joint spacing increases, the groundwater table should rise to a higher elevation to be able to have an impact. Moreover, the impact on the factor of safety is similar for different joint friction angles when the groundwater level elevation is high. This study provides a better understanding of the impact of groundwater table fluctuation on the stability of jointed rock slopes.