EDS1 Contributes to Nonhost Resistance of <i>Arabidopsis thaliana</i> Against <i>Erwinia amylovora</i>

Moreau, Manon; Degrave, Alexandre; Vedel, Régine; Bitton, Frédérique; Patrit, Oriane; Renou, Jean-Pierre; Barny, Marie‐Anne; Fagard, Mathilde

doi:10.1094/mpmi-05-11-0111

Cited by 54 publications

(76 citation statements)

References 48 publications

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“…2012). Another important gene for the SA signaling pathway, EDS1, also contributes to host, nonhost and basal resistance against bacterial pathogens (Aarts et al 1998;Glazebrook 2001;Moreau et al 2012). Apart from plant genes, several pathogen genes also determine host/nonhost status of a plant.…”

Section: Page 12 Of 37mentioning

confidence: 99%

Host Versus Nonhost Resistance: Distinct Wars with Similar Arsenals

2015

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Plants face several challenges by bacterial, fungal, oomycete, and viral pathogens during their life cycle. In order to defend against these biotic stresses, plants possess a dynamic, innate, natural immune system that efficiently detects potential pathogens and initiates a resistance response in the form of basal resistance and/or resistance (R)-gene-mediated defense, which is often associated with a hypersensitive response. Depending upon the nature of plant-pathogen interactions, plants generally have two main defense mechanisms, host resistance and nonhost resistance. Host resistance is generally controlled by single R genes and less durable compared with nonhost resistance. In contrast, nonhost resistance is believed to be a multi-gene trait and more durable. In this review, we describe the mechanisms of host and nonhost resistance against fungal and bacterial plant pathogens. In addition, we also attempt to compare host and nonhost resistance responses to identify similarities and differences, and their practical applications in crop improvement.

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Section: Page 12 Of 37mentioning

confidence: 99%

Host Versus Nonhost Resistance: Distinct Wars with Similar Arsenals

2015

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“…EDS5 is involved in the transport of precursors for SA biosynthesis (Nawrath et al 2002). EDS1 is another regulatory protein (Moreau et al 2012 ) and it controls SA production to amplify defense signals (Eulgem et al 2004;Song et al 2004). PAD4 displays similarity to triacyl glycerol lipases and other esterases (Jirage et al 1999).…”

Section: Salicylate Signaling Systemmentioning

confidence: 99%

“…SA elevates NPR1 (for non-expresser of PR gene1 ), which is a master regulator of SA-mediated defense responses . SA induces increased expression of several WRKY and ERF transcription factors in Arabidopsis (Knoth et al 2007 ;Mao et al 2007 ;Miao and Zentgraf 2007 ;Zheng et al 2006Zheng et al , 2007Zhang et al 2007d ;Grennan 2008 ;Moreau et al 2012 ). These transcription factors are necessary for the inducible expression of several defense genes (Yu et al 2001 ;Grennan 2008 ).…”

Section: Salicylate Signaling Systemmentioning

confidence: 99%

“…They play important role in activating plant immune responses either positively Qiu et al 2007 ;Moreau et al 2012 ) or negatively Nurmberg et al 2007;Kim et al 2008b ;Sun et al 2010 ;Moreau et al 2012 ). In Arabidopsis , there are 72 expressed WRKY genes, and many of them are implicated in the regulation of the plant immune responses positively or negatively via modulation of the JA/SA signaling pathways (Eulgem and Somssich 2007 ).…”

Section: Pamp-induced Expression Of Transcription Factorsmentioning

confidence: 99%

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PAMP Signaling in Plant Innate Immunity

Vidhyasekaran

2014

PAMP Signals in Plant Innate Immunity

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Plant innate immunity is a potential basal defense system existing in plant kingdom. This system provides powerful weapons to the host plants to fi ght against viral, bacterial, fungal, and oomycete pathogens and serves as a surveillance system against invasion of pathogens. It is not active in normal healthy plants and it requires specifi c signals to get activated. Pathogen-associated molecular patterns (PAMPs) act as alarm/danger signals to trigger the plant innate immune responses. When pathogens land on the plant's surface, plants read the molecular fi ngerprints/signatures of pathogens (PAMPs) by binding the PAMPs with cognate pattern-recognition receptors (PRRs) residing in plant cell plasma membrane and trigger several defense signaling systems.

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“…However, its mechanism, particularly against bacterial pathogens, is not well understood 4 . Screening for mutants or silenced plants that compromise nonhost resistance, and high throughput transcript profiling for identification of differentially expressed genes during nonhost resistance [5][6][7][8][9] are two major approaches previously used for dissecting bacterial nonhost resistance. Because nonhost resistance is controlled by a complex mechanism(s) 4 with the involvement of many genes, a high throughput functional genomic approach for gene identification is critical for better understanding the nonhost resistance mechanism(s).…”

Section: Introductionmentioning

confidence: 99%

VIGS-Mediated Forward Genetics Screening for Identification of Genes Involved in Nonhost Resistance

Senthil‐Kumar

Lee

Mysore

2013

JoVE

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Nonhost disease resistance of plants against bacterial pathogens is controlled by complex defense pathways. Understanding this mechanism is important for developing durable disease-resistant plants against wide range of pathogens. Virus-induced gene silencing (VIGS)-based forward genetics screening is a useful approach for identification of plant defense genes imparting nonhost resistance. Tobacco rattle virus (TRV)-based VIGS vector is the most efficient VIGS vector to date and has been efficiently used to silence endogenous target genes in Nicotiana benthamiana.In this manuscript, we demonstrate a forward genetics screening approach for silencing of individual clones from a cDNA library in N. benthamiana and assessing the response of gene silenced plants for compromised nonhost resistance against nonhost pathogens, Pseudomonas syringae pv. tomato T1, P. syringae pv. glycinea, and X. campestris pv. vesicatoria. These bacterial pathogens are engineered to express GFPuv protein and their green fluorescing colonies can be seen by naked eye under UV light in the nonhost pathogen inoculated plants if the silenced target gene is involved in imparting nonhost resistance. This facilitates reliable and faster identification of gene silenced plants susceptible to nonhost pathogens. Further, promising candidate gene information can be known by sequencing the plant gene insert in TRV vector. Here we demonstrate the high throughput capability of VIGS-mediated forward genetics to identify genes involved in nonhost resistance. Approximately, 100 cDNAs can be individually silenced in about two to three weeks and their relevance in nonhost resistance against several nonhost bacterial pathogens can be studied in a week thereafter. In this manuscript, we enumerate the detailed steps involved in this screening. VIGS-mediated forward genetics screening approach can be extended not only to identifying genes involved in nonhost resistance but also to studying genes imparting several biotic and abiotic stress tolerances in various plant species. Video LinkThe video component of this article can be found at

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EDS1 Contributes to Nonhost Resistance of Arabidopsis thaliana Against Erwinia amylovora

Cited by 54 publications

References 48 publications

Host Versus Nonhost Resistance: Distinct Wars with Similar Arsenals

Host Versus Nonhost Resistance: Distinct Wars with Similar Arsenals

PAMP Signaling in Plant Innate Immunity

VIGS-Mediated Forward Genetics Screening for Identification of Genes Involved in Nonhost Resistance

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