EDTA-Modified Carbapenem Inactivation Method: a Phenotypic Method for Detecting Metallo-β-Lactamase-Producing
            <i>Enterobacteriaceae</i>

Sfeir, Maroun M.; Hayden, Joshua; Fauntleroy, Kathy; Mazur, C.; Johnson, J. Kristie; Simner, Patricia J.; Das, Sanchita; Satlin, Michael J.; Jenkins, Stephen G.; Westblade, Lars F.

doi:10.1128/jcm.01757-18

Cited by 94 publications

(73 citation statements)

References 15 publications

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“…Bacterial isolates showing a positive mCIM test were cultured on Trypticase soy agar with sheep blood (TSAB). The EDTA-modified carbapenem inactivation method (eCIM) was performed according to Sfeir et al [45]. The isolates were considered positive for MBL production if the zone diameter of meropenem disc increased by ≥5 mm in comparison to the zone diameter observed for the mCIM and were considered negative for MBL production if the increase in the zone diameter was ≤4 mm.…”

Section: Edta-modified Carbapenem Inactivation Methods (Ecim) For Detementioning

confidence: 99%

The Inc FII Plasmid and its Contribution in the Transmission of blaNDM-1 and blaKPC-2 in Klebsiella pneumoniae in Egypt

et al. 2019

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The emergence of bla KPC-2 and bla NDM-1 producing Klebsiella pneumoniae represents a great problem in many Egyptian hospitals. One hundred and twenty-six K. pneumoniae isolates from patients admitted to Assiut University Hospital were identified by an API20E kit. Carbapenemase-producing K. pneumoniae (CPKP) was detected by the modified carbapenem inactivation method (mCIM), the EDTA-modified carbapenem inactivation method (eCIM), and an E-test. Based on the polymerase chain reaction, all isolates were negative for bla-VIM-1 and bla-IMP-1 , fifteen of these isolates were positive for both bla KPC-2 and bla NDM-1 , two isolates were positive for bla KPC-2 only, and twenty-eight isolates were positive for bla-NDM-1 only. Although one isolate was positive for the string test, all CPKP isolates were negative for capsular genes. Only 71.1% of CPKP transferred their plasmids to their corresponding transconjugants (E. coli J53). The resistance patterns of the clinical isolates and their transconjugates were similar, except for 12 isolates, which showed differences with their transconjugates in the resistance profile of four antibiotics. Molecular typing of the plasmids based on replicon typing showed that Inc FIIK and FII plasmids predominated in isolates and their transconjugants carrying bla KPC-2 and/or bla NDM-1 . Conjugative Inc FII plasmids play an important role in the spread of CPKP, and their recognition is essential to limit their spread.

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Section: Edta-modified Carbapenem Inactivation Methods (Ecim) For Detementioning

confidence: 99%

The Inc FII Plasmid and its Contribution in the Transmission of blaNDM-1 and blaKPC-2 in Klebsiella pneumoniae in Egypt

et al. 2019

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“…Not only detection of carbapenemases but also identification of their genes accurately and rapidly in Enterobacteriaceae is still a major challenge for clinical laboratories. The phenotypic methods such as Carba NP and modified carbapenem inactivation method (mCIM), which are recommended by Clinical and Laboratory Standards Institute (CLSI) guidelines are commonly used in routine laboratory practice (Humphries et al ; Tamma and Simner ; Sfeir et al ). Though these methods detect carbapenemase activity irrespective of carbapenemase genes, inhibitor‐based methods can be used for differentiation of carbapenemase genes (Ohsaki et al ; Greissl et al ).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of MASTDISCS combi Carba plus for the identification of metallo‐β‐lactamases, KPC and OXA‐48 carbapenemase genes in Enterobacteriaceae clinical isolates

Lü

Guo

et al. 2019

Lett Appl Microbiol

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The increasing frequency of class A KPC enzymes, class B metallo‐β‐lactamases (MBLs) and class D OXA‐48 enzymes in Enterobacteriaceae makes their early identification urgent. A simple commercial MASTDISCS combi Carba plus disc system (MAST‐Carba plus) was designed for the identification of MBLs, KPC and OXA‐48 carbapenemase genes in Enterobacteriaceae. To validate the MAST‐Carba plus, a total of 77 isolates of carbapenemase‐producing Enterobacteriaceae (CPE) and 84 isolates of noncarbapenemase‐producing Enterobacteriaceae (non‐CPE) were selected for differentiation of the genes of Enterobacteriaceae by MAST‐Carba plus. Meanwhile, the carbapenemase genes such as blaKPC, blaIMP, blaVIM, blaNDM‐1 and blaOXA‐48 were detected by PCR (polymerase chain reaction). Thus, when considered on the basis of PCR results, the sensitivity of MAST‐Carba plus detection of KPC strains is 82·3%, the specificity is 100·0%, the positive predictive value is 100·0% and the negative predictive value is 92·4%. For MBLs strains, the sensitivity is 100·0%, the specificity is 97·1%, the positive predictive value is 84·6% and the negative predictive value is 100·0%. For OXA‐48 strains, the sensitivity is 100·0%, the specificity is 99·4%, the positive predictive value is 80·0% and the negative predictive value is 100·0%. Our findings suggest that MAST‐Carba plus is a rapid and promising method for identifying the MBLs, KPC and OXA‐48 carbapenemase genes in Enterobacteriaceae, which could be exploited in basic microbiology laboratory to prevent the transmission of CPE. Significance and Impact of the Study Not only detection of carbapenemases but also identification of their genes accurately and rapidly in Enterobacteriaceae is still a major challenge for clinical laboratories in order to prevent the transmission of carbapenemase‐producing Enterobacteriaceae (CPE). Therefore, this study aimed to evaluate the performance of a new rapid method (MASTDISCS combi Carba plus) for the identification of metallo‐β‐lactamases (MBLs), KPC and OXA‐48 carbapenemase genes in Enterobacteriaceae clinical isolates.

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“…Если он является положительным, то сравнивают диаметры зон задержки роста контрольного штамма вокруг дисков с меропенемом, использованных для постановки mCIM и eCIM. В случае если диаметр зоны задержки роста в eCIM превышает на ≥ 5 мм таковой в mCIM, то тестируемый штамм признают продуцентом МБЛ [14].…”

Section: метод инактивации карбапенемовunclassified

Comparative review of the modern methods for carbapenemases detection

Popov

2019

CMAC

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Современные методы определения продукции карбапенемаз Попов Д.А. 126 КМАХ. 2019. Том 21. №2 АНТИБИОТИКОРЕЗИСТЕНТНОСТЬ * Коммерческий тест в настоящее время не зарегистрирован в РФ. Современные методы определения продукции карбапенемаз Попов Д.А. 132 КМАХ. 2019. Том 21. №2 АНТИБИОТИКОРЕЗИСТЕНТНОСТЬ * Использование карбапенемов в стандартных дозах в виде монотерапии может быть недостаточно эффективным, особенно в случае тяжелых инфекций. ** При выявлении генов blaКРС, blaОХА-48-цефтазидим/авибактам или комбинации на основе полимиксинов. При выявлении генов МБЛ-комбинации на основе полимиксинов или цефтазидим/авибактам + азтреонам.

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EDTA-Modified Carbapenem Inactivation Method: a Phenotypic Method for Detecting Metallo-β-Lactamase-Producing Enterobacteriaceae

Cited by 94 publications

References 15 publications

The Inc FII Plasmid and its Contribution in the Transmission of blaNDM-1 and blaKPC-2 in Klebsiella pneumoniae in Egypt

The Inc FII Plasmid and its Contribution in the Transmission of blaNDM-1 and blaKPC-2 in Klebsiella pneumoniae in Egypt

Evaluation of MASTDISCS combi Carba plus for the identification of metallo‐β‐lactamases, KPC and OXA‐48 carbapenemase genes in Enterobacteriaceae clinical isolates

Comparative review of the modern methods for carbapenemases detection

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