Efecto de dos medios de fertilización en el desarrollo in vitro de embriones bovinos criollos

Vargas, José Goicochea; Jorge, Wilson Rondón; Pachorro, Fidel Acosta; Marin, Yusep Gomez; Martin, Magaly Montalvo; Alor, Max Salvatierra; Falcón, Jéssica Martel; Zevallos, Oscar Ballarte; Zegarra, Julio Díaz; Fuster, Marcelo Ratto

doi:10.15381/rivep.v32i5.18709

Cited by 2 publications

(1 citation statement)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…OPU-IVF has been successful for Bos taurus breeds in the temperate climatic region and for Bos indicus breeds in the hot climatic tropical region [3,4]. However, for Bos taurus adapted breeds to the hot climatic tropical region studies are scarce and less successful [5,6],among other possible factors, the adverse hot humid climate affects directly and indirectly the reproductive performance of females [7]. The low fertility of cattle in hot climates is related to heat stress caused by high maximum temperatures (Tmax) and relative humidity (RH) [8].…”

Section: Introductionmentioning

confidence: 99%

<em>In vitro</em> embryos of Romosinuano and Tropical Milking cattle during three seasons in Veracruz, Mexico

Rosales-Martínez,

Becerril-Pérez,

Rosendo-Ponce

et al. 2024

Preprint

View full text Add to dashboard Cite

One of the main factors that influence the fertility of cattle in grazing systems in hot tropical climates is heat stress. The objective of this study was to evaluate the effect of season, breed, hormonal and physiological condition on the quantity and quality of cumulus-oocyte complexes (COCs) and embryos produced in vitro, from Romosinuano (RM) and Tropical Milking (TM) donors. Three ovum pick-up and in vitro fertilization (OPU-IVF) were performed, one per season; hot dry (HD; 10, 10), hot humid (HH; 9, 9) and fresh dry (FD; 7, 10) in RM and LT donors. Serum levels of cortisol, insulin and glucose were measured, in addition to heart rate (HR), respiratory rate (RR) and rectal temperature (RT). Effect of season x genotype interaction (p≤0.05) was observed in all COCs variables and only in cleavage embryos (CLI) (p≤0.05). Body weight (BW) affected all COCs variables (p≤0.01), except unviable (UNV) although affected degenerated embryos (DEG) (p≤0.01) and total blastocysts (BLAST) (p≤0.01). Cow age only affected viable COCs (VIAB) (p ≤ 0.05), code one blastocysts (BC1) and BLAST (p≤0.01). Cortisol affected total COCs (COCsT), VIAB and total matured in vitro (TMIV) (p≤0.01), as well as CLI, BC1 (p≤0.01) and BLAST (p≤0.05). Insulin affected COCsT (p≤0.01), UNV (p≤0.05), denuded oocytes (DE) (p≤0.01), BC1 and code two blastocysts (BC2) (p≤0.01). Glucose affected all COCs variables (p≤0.01), except UNV and all embryo variables except BC2. HR affected COCsT, DE, TMIV (p≤0.01), CLI, BLAST and DEG (p≤0.05). RR affected COCsT, UNV, VIAB, CLI (p≤0.05), BC1, BLAST and DEG (p≤0.01). RT only affected DE, VIAB (p≤0.01) and BLAST (p≤0.05). The seasonal climatic year variation of Veracruz and changes in physiological and hormonal variables have diverse effects on the cumulus-oocyte complexes and embryos produced by RM and TM donors.

show abstract

Section: Introductionmentioning

confidence: 99%

<em>In vitro</em> embryos of Romosinuano and Tropical Milking cattle during three seasons in Veracruz, Mexico

Rosales-Martínez,

Becerril-Pérez,

Rosendo-Ponce

et al. 2024

Preprint

View full text Add to dashboard Cite

show abstract

In Vitro Embryos of Romosinuano and Tropical Milking Cattle during Three Seasons in Veracruz, Mexico

Rosales-Martínez,

Becerril-Pérez,

Rosendo-Ponce

et al. 2024

Animals

View full text Add to dashboard Cite

One of the main factors that influences the fertility of cattle in grazing systems in hot tropical climates is heat stress. The objective of this study was to evaluate the effect of season, breed, hormonal and physiological condition on the quantity and quality of cumulus–oocyte complexes (COCs) and embryos produced in vitro, from Romosinuano (RM) and Tropical Milking (TM) donors. Three ovum pick-up and in vitro fertilization (OPU-IVF) were performed, one per season: hot dry (HD; 10, 10), hot humid (HH; 9, 9) and fresh dry (FD; 7, 10) in RM and LT donors. Serum levels of cortisol, insulin and glucose were measured, in addition to heart rate (HR), respiratory rate (RR) and rectal temperature (RT). Effect of season x genotype interaction (p ≤ 0.05) was observed in all COC variables and only in cleavage embryos (CLI) (p ≤ 0.05). Body weight (BW) affected all COC variables (p ≤ 0.01), except unviable (UNV) although affected degenerated embryos (DEG) (p ≤ 0.01) and total blastocysts (BLAST) (p ≤ 0.01). Cow age only affected viable COCs (VIAB) (p ≤ 0.05), code one blastocysts (BC1) and BLAST (p ≤ 0.01). Cortisol affected total COCs (COCsT), VIAB and total matured in vitro (TMIV) (p ≤ 0.01), as well as CLI, BC1 (p ≤ 0.01) and BLAST (p ≤ 0.05). Insulin affected COCsT (p ≤ 0.01), UNV (p ≤ 0.05), denuded oocytes (DE) (p ≤ 0.01), BC1 and code two blastocysts (BC2) (p ≤ 0.01). Glucose affected all COC variables (p ≤ 0.01), except UNV and all embryo variables except BC2. HR affected COCsT, DE, TMIV (p ≤ 0.01), CLI, BLAST and DEG (p ≤ 0.05). RR affected COCsT, UNV, VIAB, CLI (p ≤ 0.05), BC1, BLAST and DEG (p ≤ 0.01). RT only affected DE, VIAB (p ≤ 0.01) and BLAST (p ≤ 0.05). The seasonal climatic year variation of Veracruz and changes in physiological and hormonal variables have diverse effects on the cumulus–oocyte complexes and embryos produced by RM and TM donors.

show abstract

Efecto de dos medios de fertilización en el desarrollo in vitro de embriones bovinos criollos

Cited by 2 publications

References 11 publications

<em>In vitro</em> embryos of Romosinuano and Tropical Milking cattle during three seasons in Veracruz, Mexico

<em>In vitro</em> embryos of Romosinuano and Tropical Milking cattle during three seasons in Veracruz, Mexico

In Vitro Embryos of Romosinuano and Tropical Milking Cattle during Three Seasons in Veracruz, Mexico

Contact Info

Product

Resources

About