Efecto de la colchicina sobre la morfología foliar y los estomas de <i>Kalanchoe daigremontiana</i> Raym.-Hamet & H.Perrier (Crassulaceae)

Abstract

El presente estudio evaluó el efecto de diferentes concentraciones y tiempo de exposición de colchicina, sobre la morfología foliar y los estomas de Kalanchoe daigremontiana. Inicialmente, se recolectaron y se sometieron las plántulas de K. daigremontiana a concentraciones de 0,025% y 0,1% (p/v) de colchicina y a dos tiempos de exposición (24 y 48 horas). Posteriormente, se realizaron estudios morfológicos como la altura de la planta (AP), anchura de la hoja (AH), número de hojas (NH), longitud foliar (LH), espesor de hoja (EH) y volumen foliar (VH) cada 15 días, durante 16 semanas después de la siembra. Luego, se caracterizaron los estomas, teniendo en cuenta el ancho, el largo, índice estomático y el número de cloroplasto por estoma. Se encontró un incremento significativo en la morfología foliar y estomática, en los tratamientos con colchicina de 0,025% a 24 y 48 horas. Lo que demuestra que el uso de la colchicina logra un mayor crecimiento en poco tiempo y aumento de la biomasa en la planta medicinal K. daigremontiana.

Impact of colchicine on leaf morphology and stomatics of Kalanchoe tubiflora (Harv.) Raym.-Hamet (Crassulaceae)

“…On the other hand, height, leaves number, foliar thickness, foliar volume and num ber of seedlings with the rest of treatments (Fig. 2), the direct effect that colchicine has on the morphology could be confirmed (Salazar et al 2018); in that way, the useof 0.15%for48h,0.1%and0.15%for72hcauseda con si derableincreaseinfoliarthicknessand91.7,78.7and 100 % (respectively) of tetraploid cells in Aloe vera (Molero et al 2018). Similarly, 3 species of kumquats submitted to colchicine produced thicker, significantly rounder and heavierleaves(Nukayaetal.2019).Next,whencomparing the use of 0.05 % and 0.025 % for 24 h (T2 and T3), likewise, the use of 0.1 % and 0.05 % for 48 h (T5 and T6), it is observed that even doubling the colchicine con cen tra tionitdoesnotachieveasignificantdifferencebetween most of the variables of these treatments, which is similar toGalloneetal.…”

“…The genus Kalanchoe belongs to the Crassulaceae family which includes about 125 described species (Salazar et al 2018). Fifty six species are native to Southern and Eas tern Africawhiletheremaining60speciesarenativetoMa da gas car (Pokhrel & Karsai 2015, Vlielander et al 2018.…”

“…To idicate polyploid plants, indirect methods are performed as morphological characters (Sánchez & Matos 2012), cytological methods measuring the stomatal size and density, the number of chloroplasts in stomata guard cells (Sadatetal.2017,Orrillo&Bonierbale2009,Choqueetal. 2007,Standringetal.1990)andbychromosomecounting (Matos 2014, Salazar et al 2018. For the foregoing, taking into account the medicinal and ornamental importance, the following study evaluates the effect of different con cen trationsandexposuretimeof colchicineonfoliarmor pho logy and K. tubiflora stomata.…”

“…K. tubiflora seedlings were collected from a mother plant collected in Cúcuta municipality, Colombia. 80 seedlings were taken, with 1 cm height average and a de ve loped root system (Salazar et al 2018). Subsequently, the seedlings were submitted to different colchicine con cen tra tions (0.025, 0.05 and 0.1 % w/v) and exposure times (24 and 48 h) in total darkness, taking the distilled water as control(Table1).Next,theywereplantedin1litrepoly ethy lene bags on a substrate composed of vermiculite, rice huskandclayeysoilinproportions1:1:1.…”

“…For stomatal characteristics such as width, length and chloroplast number, the methodology developed by Salazar et al (2018), where transparent adhesive tape was used to remove the epidermis from the abaxial zone, from the central part of the sheet of approximately 1 cm 2 . The tape wasfixedonaslide,thenthespecimenwasplacedunder the LEICA compound microscope model DME 500.…”