Efectos de la criopreservación sobre las subpoblaciones espermáticas en caprinos

Corredor, Leonardo Hernández; Rodríguez, Omar Camargo; Torres, A. S.; Páez, Juan David Montoya; Moreno, Armando Quintero

doi:10.15381/rivep.v29i3.14169

Cited by 3 publications

(2 citation statements)

References 13 publications

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“…Finally, the semen pH was seven in one male and eight in the other. The ejaculation of the evaluated males was carried out in October when at the end of the rainy season and the beginning of the forage availability; however, the sperm quality of the males was lower (224106 sperm mL 1 and 16106 sperm mL 1 ) than that established for this species (2,500 to 3,000106 sperm mL 1 ) (Hernández-Corredor et al, 2018). This can be explained in the following way (first) the males were recovering from the dry season where there was little available food, followed by the moment when the males were ejaculated, who had finished the riding period, and finally, for a male to ejaculate, previous training is needed to reduce their stress level.…”

Section: Resultsmentioning

confidence: 96%

Reproductive evaluation of bucks (Capra hircus L.) with usual management in herds from Benito Juarez, Guerrero, Mexico

Hernández-Ruiz

González

Pineda-Burgos

et al. 2021

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Objective: To evaluate the reproductive characteristics of male goats (Capra hircus L.)with habitual management on their herds at Benito Juarez municipality, Guerrero, Mexico.Design / methodology / approach: Ten male Creole goats from seven herds wereevaluated, aged between 2 to 7 years and 2 to 3 body condition (BC). The males wereevaluated regarding their sexual behavior, sperm quality, physical examination andreproductive clinical evaluation.Results: All the assessed male Creole goats displayed sexual behavior (P < 0.05).However, for the males of three of the evaluated herds the sexual behavior was lower (P < 0.05). Of the ten electro-ejaculated males, 20% ejaculated and 80% did not (P < 0.05). From the males that ejaculated, a sperm concentration of 224 x 106 and 16 x 106 spermmL -1 was recorded for the first and second male. Finally, the physical and clinicalreproductive evaluation concurred with the established parameters for the species.Limitations of the study / implications: Under the conditions in which the study wascarried out, it was not possible to evaluate males in the same age conditions andreproductive characteristics. However, this allowed collecting information from fieldconditions so that producers can apply it for herd improvement.Findings / conclusions: The male Creole goats of the evaluated herds showed intensesexual behavior with low sperm characteristics.

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Section: Resultsmentioning

confidence: 96%

Reproductive evaluation of bucks (Capra hircus L.) with usual management in herds from Benito Juarez, Guerrero, Mexico

Hernández-Ruiz

González

Pineda-Burgos

et al. 2021

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“…47 The differences in the size of the head between the sperm in the different treatments occur because the sperm has modified the function of the membrane and this sperm will have lost or gained part of their intracellular content as a result of the osmotic change. 48 This explains the excessive growth of the sperm cells in the face of physical-chemical changes and the physiological adaptation of the cells to the different stressors of the components that add them in each process. It should be noted that the treatment of EPp and EPr have higher values than those taken in the fresh sample, this can be justified in the role that the biopharmaceutical performs when included in sheep semen.…”

Section: Sperm Motility Depends On Endogenous and Exogenous Factorsmentioning

confidence: 99%

Effect of the incorporation of plasma rich of platelets on the spermatozoa physiology of ram semen

Corredor L,

Restrepo S,

Cano J

et al. 2020

JDVAR

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Plasma rich in platelets (PRP) have been used for healing processes, maxillary oral surgery, plastic surgery, although its use in andrology is null; however, there is no information about its effect on sperm motility and morphology. The aim of this study was to compare the effect PRP in inclusion ram semen in the parameters motility (Mt) and morphometrics (Mp). Semen samples (n=8) were collected by artificial vagina from two rams located in the North of Santander, Colombia (L 7°54′N, L 72°30′W). Obtain the blood sample by venous puncture of the jugular the tubes (Citrated and EDTA) at 3000 g/3 min centrifuged, after separation PRP (rich and poor). After the initial evaluation, Eppendorf tubes of 2mL, 500μL, saline solution, 10μL of fresh semen and 10μL PRP (rich or poor) were placed 5 min after preparation of the samples, (F:fresh, N: saline solution, CPr: citrated platelets rich; CPp: citrated platelets poor; EPr: EDTA platelets rich; EPp EDTA platelets poor). sperm was analyzed per software the Sperm Class Analyser (ISAS, Proiser, Spain) were calculated for Mp and for Mt, parameters were compared between treatments (T) by ANOVA. Significant differences (P<0.001) were found between T for Mp and Mt. According to these results, the sperm head dimensions (SHD) of semen samples with inclusion EPr were bigger and the velocity was higher. An increase in SHD has been previously described due to osmolar adjustment procedures, the same happened with the parameter of Mt and linearity, improving the characteristic up to 6% with respect to fresh semen. it is observed that the PRP improves the Mp and the Mt of the ram spermatozoa, thanks to the growth factors and the components of its dense granules

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Red-Light Irradiation of Horse Spermatozoa Increases Mitochondrial Activity and Motility through Changes in the Motile Sperm Subpopulation Structure

Catalán

Papas

Gacem

et al. 2020

Biology

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Previous studies in other mammalian species have shown that stimulation of semen with red-light increases sperm motility, mitochondrial activity, and fertilizing capacity. This study sought to determine whether red-light stimulation using a light emitting diode (LED) at 620–630 nm affects sperm motility and structure of motile subpopulations, sperm viability, mitochondrial activity, intracellular ATP levels, rate of O2 consumption and DNA integrity of horse spermatozoa. For this purpose, nine ejaculates were collected from nine different adult stallions. Upon collection, semen was diluted in Kenney extender, analyzed, its concentration was adjusted, and finally it was stimulated with red-light. In all cases, semen was packaged in 0.5-mL transparent straws, which were randomly divided into controls and 19 light-stimulation treatments; 6 consisted of a single exposure to red-light, and the other 13 involved irradiation with intervals of irradiation and darkness (light-dark-light). After irradiation, sperm motility was assessed using a Computerized Semen Analysis System (CASA). Flow cytometry was used to evaluate sperm viability, mitochondrial membrane potential and DNA fragmentation. Intracellular levels of ATP and O2 consumption rate were also determined. Specific red-light patterns were found to modify kinetics parameters (patterns: 4, 2-2-2, 3-3-3, 4-4-4, 5-1-5, and 5-5-5 min), the structure of motile sperm subpopulations (patterns: 2, 2-2-2, 3-3-3, and 4-1-4 min), mitochondrial membrane potential (patterns: 4, 3-3-3, 4-4-4, 5-1-5, 5-5-5, 15-5-15, and 15-15-15 min), intracellular ATP levels and the rate of O2 consumption (pattern: 4 min), without affecting sperm viability or DNA integrity. Since the increase in some kinematic parameters was concomitant with that of mitochondrial activity, intracellular ATP levels and O2 consumption rate, we suggest that the positive effect of light-irradiation on sperm motility is related to its impact upon mitochondrial activity. In conclusion, this study shows that red LED light stimulates motility and mitochondrial activity of horse sperm. Additional research is needed to address the impact of red-light irradiation on fertilizing ability and the mechanisms through which light exerts its effects.

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Efectos de la criopreservación sobre las subpoblaciones espermáticas en caprinos

Cited by 3 publications

References 13 publications

Reproductive evaluation of bucks (Capra hircus L.) with usual management in herds from Benito Juarez, Guerrero, Mexico

Reproductive evaluation of bucks (Capra hircus L.) with usual management in herds from Benito Juarez, Guerrero, Mexico

Effect of the incorporation of plasma rich of platelets on the spermatozoa physiology of ram semen

Red-Light Irradiation of Horse Spermatozoa Increases Mitochondrial Activity and Motility through Changes in the Motile Sperm Subpopulation Structure

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