ABSTRACT(Responses in germination and early growth of radish under the infl uence of an aqueous extract of Piper mikanianum (Kunth) Steudel). Th is study evaluated the allelopathic eff ects of an extract from P. mikanianum leaves on the germination and early growth of radish (Raphanus sativus L.). Leaf extracts were prepared at concentrations of 2, 4 and 8%. Th e pH and osmotic potential of the extracts were determined. Germination bioassay consisted of fi ve replicates of 25 radish seeds distributed in Petri dishes with germitest paper and 7 mL of extract or water, and were kept at 25°C in B.O.D. for fi ve days. Germination percentage (PG), germination speed (VG), germination speed index (IVG) and index of allelopathic eff ect (RI) were determined. For bioassay initial growth, seeds were germinated until the protrusion of the radicle was 2 mm and transferred to Gerbox boxes containing germitest paper and 15 mL of extract or water, which were kept for seven days at 25°C in B.O.D. to evaluate the length of the radicle and hypocotyl, fresh and dry mass, water content and chlorophyll content (a, b and total). Leaf extracts from P. mikanianum negatively aff ected all parameters examined for germination (PG, VG, IVG and RI). Th e extracts aff ected the initial growth, causing reduction of the radicle length and stimulating the growth of the hypocotyl. In addition, the extracts at 8% concentration reduced fresh weight and water content. Extracts at concentrations of 2 and 4% increased the content of chlorophyll b and total chlorophyll. Th us, Piper mikanianum exerted an allelopathic eff ect on radish.