Efeito da adição do ácido linoleico conjugado no cultivo in vitro de embriões F1 Holandês x Zebu na sobrevivência pós-vitrificação

Leite, A. C.; Andrade, V. B.; Silva, Eliane Beatriz Magalhães; Borges, Álan Maia

doi:10.1590/1678-4162-9238

Cited by 8 publications

(5 citation statements)

References 11 publications

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“…All photos were converted to black and white (32 bt), and the oocytes included in antral follicles were delimited and analyzed using the area of integrated density compared. This evaluation was performed in the Image J ® software according to Leite et al (2017) .…”

Section: Methodsmentioning

confidence: 99%

Cis-9, trans-11 and trans-10, cis-12 conjugated linoleic acid gastric administration during the pregestational and gestational periods does not influence the follicular endowment of the progeny

Freitas,

Lopes,

Nascimento

et al. 2023

Anim. Reprod.

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Fetal programming suggests that maternal stimulation and nutrition during the period of fetal development can program the progeny. Conjugated linoleic acid (CLA), an isomer of linoleic acid, has been characterized in several aspects, but few studies have been performed on its involvement in reproduction and fetal programming. The aim of this study was to evaluate the F1, F2 and F3 progeny of female mice supplemented with CLA during the pregestational and gestational periods with respect to biometric and reproductive parameters, as well as ovarian morphophysiology. The F1 progeny of mothers supplemented with CLA exhibited stable weight gain, while the F2 progeny showed no effects (P=0.0187 and P=0.0245, respectively). A reduction in Lee's Index was observed in both generations at the second post-weaning evaluation week in the animals treated with CLA (P=0.0100 and P=0.0078, respectively). The F2 generation showed an increase in the anogenital index in both sexes of the animals treated with CLA (P= 0.0114 and P<0.0001, female and male respectively). CLA administration to mothers did not affect any of the following in their progeny: ovarian follicle mobilization (P>0.05), follicle number (P>0.05) and the integrated density of the lipid content of oocytes included in antral follicles (P>0.05). This study evaluated the use of CLA in mothers and found that it did not affect the progeny regarding murine reproductive performance, suggesting that this supplement can be used safely.

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Section: Methodsmentioning

confidence: 99%

Cis-9, trans-11 and trans-10, cis-12 conjugated linoleic acid gastric administration during the pregestational and gestational periods does not influence the follicular endowment of the progeny

Freitas,

Lopes,

Nascimento

et al. 2023

Anim. Reprod.

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show abstract

“…Five in vitro embryo culture routines were performed, considering each routine as a repeat and for each repeat it was used five to six replicates. All procedures for in vitro embryo production were based on Leite et al 21 . Briefly, small ovarian follicles containing immature oocytes were punctured to obtain cumulus-oocyte complexes (COCs).…”

Section: Methodsmentioning

confidence: 99%

In vitro toxicity of Niedenzuella (Tetrapterys) multiglandulosa on bovine embryos

et al. 2022

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Niedenzuella (Tetrapterys) multiglandulosa, a vine plant found in Brazil, has been correlated to outbreaks of poisoning in cattle and buffaloes, generating economic losses related to the death due to heart failure, miscarriage, abortion, stillbirth, and neonatal mortality. The aim of this study was to examine the embryotoxic potential of the aqueous plant extract on in vitro bovine embryos. In vitro study was performed in five replicates of bovine embryo culture assigned in two groups: control, in vitro embryo culture medium without the aqueous plant extract; treated group, with addition of 2.7mg/mL of aqueous plant extract (10%) to the embryo culture on the sixth day of culture. Cleavage rate was evaluated at day 2 of the cell culture. Viability, hatchability and underdevelopment of blastocysts on the seventh, eighth, and ninth days (D7, D8, and D9, respectively) of culture were assessed under stereoscopic microscope. On day 7, blastocysts were submitted to TUNEL assay to determine apoptotic index. In vitro exposure of bovine embryos to of N. multiglandulosa resulted in reduced embryo development and survival, evaluated by dark cytoplasm indicating poor morphology and poor quality with marked reduction of hatchability. We observed a significant reduction of blastocyst production/number of cleaved embryos (60.6% vs 41.5%); reduction of blastocysts production/total number of matured bovine oocytes (35.1% vs 21.3%); and embryonic hatching rates (38.0% vs 10.0%). However, no effects were observed on the apoptotic rate. In conclusion, aqueous extract of N. multiglandulosa leaves reduces bovine embryo viability in vitro, suggesting possible detrimental effects on embryo development.

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“…Lipid accumulation is associated with loss of embryonic viability and an increase in lesions caused by cryopreservation, especially in the early stages of embryonic development (80) . Leite et al (41) . showed that the supplementation of culture media with trans-10 conjugated linoleic acid isomer, cis-12 reduced the deposition of intracytoplasmic lipids in embryonic cells.…”

Section: Effect Of Cla Supplementation On Embryo Cryopreservationmentioning

confidence: 98%

“…One of the main effects of CLA supplementation on embryo cryopreservation is the reduction in the expression of enzymes, caused by chemical agents responsible for triglyceride catalysis, reducing lipid exposure in embryonic cells (6) . Additionally, Leite et al (41) proposed that the addition of CLA in the culture medium can affect enzymatic digestion of the zona pellucida, altering the hatching rate of embryos.…”

Section: Cla Regulates Cell Membrane Functionalitymentioning

confidence: 99%

Conjugated linoleic acid as a potential bioactive molecule to modulates gamete and embryo cryotolerance

et al. 2020

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Conjugated linoleic acid (CLA) is a mixture of positional isomers of linoleic acid found in meat and dairy products from ruminants. It is a trans fat widely used by athletes as a food supplement, due to a supposed effect of maximizing the use of body fat reserves. The interest in diet and culture media supplementation with CLA is an emerging area, demanding studies in order to elucidate its benefits in the reproductive parameters, as well as in cryopreservation. Therefore, the aim of this review was to discuss the effects of CLA on the oocytes, sperm and embryos cryotolerance. Some studies have already demonstrated its use in cryopreservation of germline. Among those, it was observed that CLA supplementation during oocyte in vitro maturation can increase their viability post-freezing and developmental capacity. Regarding the use of CLA on sperm, there are few studies and their results are still inconclusive. Finally, studies about CLA supplementation on embryo culture media have shown promising results, indicating that this bioactive molecule is able to modulate lipid uptake on blastomeres. Altogether, these findings demonstrate the potential use of CLA as a bioactive molecule to improve germline and embryo cryotolerance and open new perspectives on human and animal reproduction field.

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Efeito da adição do ácido linoleico conjugado no cultivo in vitro de embriões F1 Holandês x Zebu na sobrevivência pós-vitrificação

Abstract: Efeito da adição do ácido linoleico conjugado no cultivo in vitro de embriões F1 Holandês x Zebu na sobrevivência pós-vitrificação

Cited by 8 publications

References 11 publications

Cis-9, trans-11 and trans-10, cis-12 conjugated linoleic acid gastric administration during the pregestational and gestational periods does not influence the follicular endowment of the progeny

Cis-9, trans-11 and trans-10, cis-12 conjugated linoleic acid gastric administration during the pregestational and gestational periods does not influence the follicular endowment of the progeny

In vitro toxicity of Niedenzuella (Tetrapterys) multiglandulosa on bovine embryos

Conjugated linoleic acid as a potential bioactive molecule to modulates gamete and embryo cryotolerance

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