Efeito de Schinus terebinthifolius Raddi (aroeira) e Carapa guianensis Aublet (andiroba) na cicatrização de gastrorrafias

Abstract

-Background -The gastrorraphy isolated or associated with the use of biological adhesives formed throughout the history of surgery the usual way to promote healing in gastric lesions; however, the use of herbal medicine has been increasingly employed to help the wound healing. Aim -To evaluate the wound healing caused in the stomach of rats using extract of Schinus terebinthifolius Raddi and Carapa guianensis Aublet oil. Methods -Ninety rats, adult males were divided into three groups: aroeira, andiroba and control group, which were subdivided into three subgroups of five animals according to the time of the deaths (seven, 14 and 21 days). All underwent the same surgical procedure (injury and suture the stomach) differing only to the animals in groups aroeira and andiroba that received a daily dose of 100 mg / kg of hydroalcoholic extract and oil, by gavage, while the control group received normal saline. The parameters evaluated were the macroscopic and microscopic test of resistance to air insufflation and test the traction force.Results -All animals showed good healing of gastric and abdominal wall without infection and dehiscence. Both groups presented neighboring organs adhesions on the gastric surface. The endurance test for air insufflation showed higher average pressure within seven days and the test revealed greater traction force of rupture between seven and 14 days in groups aroeira and andiroba. The intensity of chronic inflammation revealed statistically significant differences in angiogenesis and fibroblast proliferation. Conclusion -The use of extract of Schinus terebinthifolius Raddi and Carapa guianensis Aublet oil favored the gastric wound healing in rats.RESUMO -Racional -A gastrorrafia isolada ou associada ao uso de adesivos biológicos constituiu ao longo da história da cirurgia a forma usual de promover a cicatrização nas lesões gástricas; entretanto, o uso de fitoterápico tem sido cada vez mais empregado para auxiliar o processo cicatrical. Objetivo -Avaliar a cicatrização de ferida provocada no estômago de ratos com uso do extrato hidroalcoólico de Schinus terebinthifolius Raddi e óleo da Carapa guianensis Aublet. Métodos -Foram utilizados 90 ratos, adultos, machos, distribuídos em três grupos: grupo aroeira, grupo controle e grupo andiroba, os quais foram subdivididos em três subgrupos de cinco animais conforme o momento da morte induzida (sete, 14 e 21 dias). Todos foram submetidos ao mesmo procedimento cirúrgico (lesão e rafia do estômago) diferindo apenas que os animais dos grupos aroeira e andiroba receberam dose diária de 100 mg/kg do extrato hidroalcoólico e do óleo, via gavagem, enquanto o grupo controle recebeu solução salina isotônica. Os parâmetros avaliados foram: alterações macroscópicas e microscópicas, teste de resistência à insuflação de ar atmosférico e teste pela força de tração. Resultados -Todos os animais demonstraram boa cicatrização da parede abdominal e das gastrorrafias, sem infecção e deiscência. Ambos os grupos apresentaram aderências à superfície das ga...

Schinus terebinthifolius raddi (Aroeira) and Orbignya phalerata mart. (Babassu) effect in cecorrahphy healing in rats

“…Unlike the study by Santos et al 25 in which the rupture site on insufflation test occurred in specific point of the suture line (weakest point), it can be inferred that in this site young collagen predominated; in this study, rupture occurred both inside and outside of suture site, with a significant difference in GA at day 21, when all were outside.…”

“…In relation to fibroblast proliferation, there was a significant difference at 14 days between the GC and GB, favoring GC and GA, similar to other studies 24,25 . Another important variable to assess the chronic inflammatory process is fibrosis or collagen deposition.…”

“…Studies of Lucena et al 9 and Santos et al 25 showed intensity on day 7 of chronic inflammation favoring the experimental group with a significant difference in angiogenesis variable. In the present study, there was significant difference on day 14 in GC and GA. Santos et al 24 found no difference between groups.…”

“…Already the oral route of administration by daily gavage 24,25 differs from other papers 23,26 using it parenterally, intraperitoneally and even topically in different dosages, proving that this herbal can be used by different administration routes. Was considered by the authors of this study that daily administration would give better action opportunity and could contribute to the literature with alternative higher dosage.…”

Schinus terebinthifolius Raddi ( Aroeira) leaves oil attenuates inflammatory responses in cutaneous wound healing in mice

“…It has been used in folk medicine as teas, infusions or tinctures as an anti-inflammatory, febrifuge, analgesic, and depurative agent; also, it has been used to treat skin wounds, repair injury in respiratory, digestive and genitourinary systems [1][2][3][4][5][6] . Previous reports have demonstrated that S. terebinthifolius extracts or fractions is rich in polyphenols, which display antioxidant, antibacterial, antifungal and anti-allergic activities in different experimental models [7][8] .…”