Efeitos de citocininas sobre a anatomia foliar e o crescimento de Annona glabra L. durante o cultivo in vitro e ex vitro

Oliveira, Lenaldo Muniz de; Paiva, Renato; Aloufa, Magdi Ahmed Ibrahim; Castro, Evaristo Mauro de; Santana, José Raniere Ferreira de; Nogueira, Raírys Cravo

doi:10.1590/s0103-84782008000500040

Cited by 7 publications

(8 citation statements)

References 5 publications

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“…However, at 10 µM KIN is superior to other cytokinins (10 µM BAP and TDZ) ( Figure 2D to F), and the palisade parenchyma presents more elongated cells, characterizing more markedly the mesophyll limit of the mesophyll and demonstrating greater differentiation. Oliveira et al (2008) also verified that leaves of plants subjected to treatment with BAP and KIN presented greater differentiation of the mesophyll compared to the treatments with TDZ and ZEA, with reduced proportion of intercellular spaces; this suggests higher efficaciousness of these two sources of cytokinin on the development of the assimilatory system of the plants during cultivation in vitro. The data from the aforementioned author corroborates that described for the present work at 120 days, in which the treatments with BAP and KIN at 5 and 10 µM demonstrated greater differentiation between the parenchyma.…”

Section: Anatomic Evaluationssupporting

confidence: 57%

“…Since one of the main problems of acclimatization is related to the low photosynthetic rates, the excess of cytokinins may hamper this step. In accordance with Oliveira et al (2008), in their work with pond-apple (Annona glabra -Annonaceae) using different cytokinins in the concentration of 5 µM, the thickness of the mesophyll was affected by the presence of BAP and KIN, and not by TDZ and ZEA (zeatin). This result diverges from that obtained in the present work at 120 days, in which only the presence of TDZ differed from the control.…”

Section: Anatomic Evaluationsmentioning

confidence: 53%

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Leaf anatomy of Crambe abyssinica Hochst. during in vitro shoot induction

Werner¹,

Milanez²,

Gontijo³

et al. 2016

Afr. J. Biotechnol.

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This study aimed to characterize and evaluate possible modifications in the leaf anatomy of crambe during the process of shoot induction based on micropropagation protocol. The anatomic characteristics of the leaves, and also the morphological characteristics of crambe plantlets, were evaluated during the shoot induction phase of the micropropagation. The shoots were induced by the cytokinins, 6-benzylaminopurine (BAP), kinetin (KIN) and thidiazuron (TDZ), at distinct concentrations on Murashige and Skoog (MS) medium during 120 days of culture. Comparing the leaf anatomy, it was observed that, at day 30, only the adaxial epidermis and the palisade parenchyma presented significant differences in relation to the tested concentrations, independently of the type of cytokinin employed. At 120 days, the anatomic analysis of the mesophyll demonstrated no marked difference among the cytokinins at 5 µM. Therefore, the various sources and concentrations of cytokinins applied in this work did not promote marked changes in the sense of altering the organization and/or thickness compared to the control.

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Section: Anatomic Evaluationssupporting

confidence: 57%

Section: Anatomic Evaluationsmentioning

confidence: 53%

Leaf anatomy of Crambe abyssinica Hochst. during in vitro shoot induction

Werner¹,

Milanez²,

Gontijo³

et al. 2016

Afr. J. Biotechnol.

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“…According to Aloni et al (2006), auxins and cytokinins control the differentiation of xylem and phloem, and other hormones, like gibberellins and ethylene, may also be involved in this process. Assays demonstrated that the addition of BAP along with KIN in the culture medium increased the thickness of parenchyma cells, both spongy and palisade, and consequently the thickness of foliar limbs in Annona glabra (Oliveira et al, 2008). The quantitative analysis of leaf tissues of two species in Bromeliaceae showed that 0.5 mg/L of BAP resulted in a greater distance between the xylem and phloem (Galek and Kukulczanka, 1996).…”

Section: Introductionmentioning

confidence: 99%

Growth regulators, DNA content and anatomy in vitro-cultivated Curcuma longa seedlings

Antoniazzi¹,

Ferrari²,

Nascimento³

et al. 2016

Afr. J. Biotechnol.

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Curcuma longa L., from the Zingiberaceae family, generally reproduces through its rhizomes, which are also utilized for therapeutic purposes because they are rich in terpenoids. Its conventional propagation has low efficiency due to the small number of seedlings and their contamination by pathogens. Therefore, this study aimed to evaluate the influence of growth regulators on the development of in vitro-cultivated C. longa as well as to determine their influence on DNA content and foliar anatomy. Shoots were inoculated in MS culture medium with the addition of 30 g/L of sucrose and 6.5 g/L of agar, and a pH adjusted to 5.8. Two assays were built to study the multiplication and rooting phases of growth. The first assay evaluated the influence of eight concentrations of cytokinins and auxins on the multiplication phase. Leaf samples were analyzed for DNA content through flow cytometry, utilizing two reference standards, green pea, and tomato. Characteristics of leaf anatomy were also measured in four time periods. The second assay analyzed the influence of six auxin concentrations on the rooting phase. The first assay showed that the root systems grew more in treatment 3 (4.44 µM benzylaminopurine [BAP], 0.46 µM kinetin [KIN]) and reached greater dry mass in T8 (8.88 µM BAP, 0.92 µM KIN, 2.16 µM naphthalene acetic acid [NAA]). The largest fresh matter of the main shoot was found in T2 (4.44 µM BAP). The estimated DNA content varied depending on the presence of supplemental growth regulators, from 2.38 to 2.77 pg, and was greater in T4 (4.44 µM BAP, 1.08 µM NAA) and T5 (4.44 µM BAP, 0.46 µM KIN, 1.08 µM NAA). Results from the latter two treatments were not significantly different. Estimates of DNA content were precise, as indicated by coefficients of variation that were much lower than 5%. The results also showed quantitative variation of evaluated anatomical traits. In general, there was a thin epidermis layer with rectangular cells, followed by parenchyma with octahedral cells and differentiated xylem and phloem. In leaf parenchyma, the presence of idioblasts containing phenolic compounds was observed in all growth stages. In the rooting phase, the supplementary auxins affected the dry matter of the aerial part and roots. The highest averages were observed in treatments with 2.0 µM NAA.

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“…Annona glabra L. (Annonaceae) is a fruit species that has been extensively researched due to its favorable agronomic characteristics, serving as a grafting stock for several cultivated Annonaceae (Oliveira et al 2007) due to its phytochemical properties, with antibacterial, antifungal, insecticidal and cytotoxic effects (Padmaja et al 1995;Oliveira et al 2008). However, the commercial exploitation of this species as a rootstock for cultivated Annonaceae, and as a source of bioactive compounds or even for the recovery of degraded areas of vegetation has been limited by the difficulty of obtaining healthy specimens, especially in large quantities (Hoffmann et al 1996).…”

mentioning

confidence: 99%

“…Clonal propagation via in vitro culture has been a viable alternative for the multiplication of several species (e.g., Azevedo et al 2008;Castro et al 2008;Schiavinato et al 2008;Victorio et al 2008), including A. glabra (Oliveira et al 2007;Santana et al 2008a,b). However, obtaining an efficient protocol for in vitro multiplication of Annonaceae on a commercial scale is still a challenge.…”

mentioning

confidence: 99%

Influence of flask sealing and activated charcoal on the morphogenesis and leaf anatomy of Annona glabra (Annonaceae) cultured in vitro

Santana¹,

Paiva²,

Oliveira³

et al. 2011

Sitientibus sér. Ci. Biol.

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This study evaluated the effects of the type of tube closure and the use of activated charcoal in the morphogenesis and leaf anatomy of Annona glabra (Annonaceae). Nodal segments of A. glabra were inoculated in WPM medium supplemented with 30 g l-1 sucrose and five concentrations of activated charcoal (0, 1, 2, 3 and 4 g l-1). During in vitro culture, nodal segments were kept in test tubes closed in one of three ways: plastic cap sealed with PVC wrap, plastic cap without a PVC wrap and cotton plug. The results showed that the shoots obtained in tubes closed with a plastic cap without a PVC wrap or sealed with a cotton plug had increased growth compared to those obtained in tubes sealed with a plastic cap and a PVC wrap. The presence of activated charcoal caused a reduction of leaf abscission and influenced the anatomical development of the shoots in the tubes sealed with a cotton plug and sealed with a plastic cap without a PVC wrap. The highest means for leaf tissue thickness were obtained from the shoots kept in closed tubes with a plastic lid and a PVC wrap in the absence of activated charcoal.

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Efeitos de citocininas sobre a anatomia foliar e o crescimento de Annona glabra L. durante o cultivo in vitro e ex vitro

Abstract: RESUMO O efeito de diferentes fontes de citocininas durante o cultivo in vitro de

Cited by 7 publications

References 5 publications

Leaf anatomy of Crambe abyssinica Hochst. during in vitro shoot induction

Leaf anatomy of Crambe abyssinica Hochst. during in vitro shoot induction

Growth regulators, DNA content and anatomy in vitro-cultivated Curcuma longa seedlings

Influence of flask sealing and activated charcoal on the morphogenesis and leaf anatomy of Annona glabra (Annonaceae) cultured in vitro

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