Effect Invitro Activation on the Spermiograms of ligozoospermic Patients

Abstract

Objective: The objective of this study was to improve certain sperm function parameters in vitro of oligozoospermic patients and other mild male factors using sperm activation techniques such as swim-up and density gradient. Methodology: The study was carried out between January 2014 and October 2014 including (30) semen samples of oligozoospermic patients who attended the Fertility Center in AL- Sadr Medical City. The liquefied semen was then carefully mixed for few second and the semen was analyzed by macroscopic and microscopic examination. Two method of IUI sperm activation have been used in this study to separate the motile spermatozoa in seminal plasma. Data analyzed using the SPSS version(20) Results: The results of the study showed that there was statistical significant ( p<0.05) decrease in the sperm concentration, leukocyte concentration, malondialdehyde(MDA) concentration and sperm chromatin maturity after activation by both swim-up and density gradient techniques compared with before activation. Also the results was appeared a significant (p<0.05) improvement in progressive motility (A+B) and normal morphology after activation by both techniques while, there was a significant (p<0.05) improvement in progressive motility (A+B) was noticed by using density gradient technique compared to swim-up technique. Conclusions: It was concluded that the density gradient and swim-up techniques were appeared clear improvement in the certain sperm parameters of oligozoospermic patients after activation. Recommendation: The density gradient techniques with intrauterine insemination to treatment Oligozoospermic patients.