Effect of 3-nitropropionic acid inducing oxidative stress and apoptosis of granulosa cells in geese

Abstract

The mechanism of action by which oxidative stress induces granulosa cell apoptosis, which plays a vital role in initiating follicular atresia, is not well understood. In the present study, the effect of 3-nitropropionic acid (3-NPA) on oxidative stress and apoptosis in granulosa cells in geese was investigated. Our results showed that treatment with 3-NPA at 5.0 mmol/l for 24 h increased intracellular reactive oxygen species (ROS) production by 25.4% and decreased granulosa cell viability by 45.5% (P<0.05). Catalase and glutathione peroxidase gene expression levels in granulosa cells treated with 3-NPA were 1.32- and 0.49-fold compared with those of the control cells, respectively (P <0.05). A significant decrease in the expression level of B-cell lymphoma 2 (Bcl-2) protein and remarkable increases in the levels of Bax, p53 and cleaved-Caspase 3 proteins and the ratio of Bax/Bcl-2 expression in granulosa cells treated with 3-NPA were observed (P<0.05). Furthermore, a 38.43% increase in the percentage of early apoptotic cells was also observed in granulosa cells treated with 3-NPA (P<0.05). Moreover, the expression levels of NF-κB, Nrf2, Fhc, Hspa2 and Ho-1 in granulosa cells treated with 3-NPA were elevated 4.36-, 1.63-, 3.62-, 27.54- and 10.48-fold compared with those of the control cells (P<0.05), respectively. In conclusion, the present study demonstrates that treatment with 3-NPA induces ROS production and apoptosis and inhibits the viability of granulosa cells in geese. Furthermore, 3-NPA triggers increases in the expression of cleaved-Caspase 3 protein and the ratio of Bax/Bcl-2 expression, and induces the early apoptosis of granulosa cells.

Antioxidant Defence Systems and Oxidative Stress in Poultry Biology: An Update

“…Iron dietary supplementation at 700 or 1400 mg Fe/kg was shown to significantly increase Nrf2 gene expression in jejunum of 21 day old Chinese fast growing Yellow broilers [280]. The expression levels of Nrf2 and HO-1 in goose granulosa cells treated with 3-nitropropionic acid were elevated 1.63 and 10.48 fold, respectively [281]. The mRNA level of Nrf2 in the liver and jejunum of broilers fed a diet containing soybean meal (SBM) heated at 100 • C for 8 h was significantly decreased in comparison to birds fed the control diet containing untreated SBM for 42 days [282].…”

Molecular Mechanisms Linking Oxidative Stress and Diabetes Mellitus

“…It can also induce apoptotic processes in the pancreatic cells leading to death and loss of beta cells [48,55,56]. A series of proapoptotic agents are highly sensitive to oxidative stress and can activate the apoptotic process in the pancreatic cells [63][64][65]. Moreover, an overload of free radical species has a negative effect on metabolic pathways in the beta cells and impairs K ATP channels leading to lower insulin secretion [48,61] (Figure 1).…”

Quercetin with lycopene modulates enzymic antioxidant genes pathway in isoproterenol cardiotoxicity in rats

“…Consequently, it could affect the permeability of mitochondrial and cytoplasmic membranes and release oxidative products into circulation. TBARS is a well-established index of MDA content, an oxidative stress marker for lipid peroxidation in tissues (Kang et al, 2018 ). Also, GSSG, an important oxidative tripeptide thiol that depicts the oxidative stress status in various inflammation related disorders (Shahsavani et al, 2010 ).…”