Abstract:& To selective adsorption of glucoamylase (GA) from the raw materials of the unpasteurized sake, various polymer beads were prepared by immobilization of cationic or anionic functional groups onto cellulose beads (Cellufine). Under conditions which mimic sake (GA 500 lg mL À1 , ethanol 20 vol%, pH 4), Aspergillus species GA (As-GA) (pI 3.5) was strongly adsorbed by cationic Cellufines with anion-exchange capacity (AEC) of 0.5-1.3 meq g À1 , but Rhizopus sp. GA (pI 6.5) was strongly adsorbed by anionic adsorben… Show more
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