Effect of altering the intervals between consecutive superovulatory doses of porcine follicle-stimulating hormone on ovarian responses and embryo yields in anestrous ewes

Abstract

The effect of varying intervals between successive gonadotropin injections on the superovulatory outcomes in anestrous Rideau Arcott ewes superstimulated for ovarian follicular development with multiple doses of porcine FSH (pFSH) was evaluated in a single study. Twenty-five animals received six (1×2.5ml and 5×1.25ml) injections of Folltropin-V given at 0800 and 1600h or at 0800 and 2000h in Group 1 (n=9) or Group 2 (n=16), respectively. An i.m. injection of 500 IU of equine chorionic gonadotropin (eCG; Folligon) was given concurrently with the first pFSH dose. Time of estrus was synchronized among ewes with intravaginal sponges containing 60mg of medroxyprogesterone acetate (Veramix) that were left in place for 14days; sponges were removed at the time of the 5th pFSH injection. Six days after insertion of MAP sponges, all ewes received an i.m. injection of estradiol-17β dissolved in 1ml of sesame oil (350μg/ewe) to synchronize follicular wave emergence. Following the last pFSH dose, all animals were given a single i.m. injection of 50μg of gonadotropin-releasing hormone (GnRH; Cystorelin) to induce ovulations before placing in a pen with four fertile rams for 36h. The ovarian responses were assessed and embryos recovered surgically 7days after GnRH injections. The mean number of corpora lutea was greater (P<0.05) in Group 1 compared with Group 2 ewes (21.0±2.9 compared with 10.4±1.6, respectively; mean±SEM) but there was no difference (P>0.05) in the number of transferable embryos (5.4±2.4 compared with 5.4±1.3/ewe, respectively), and Group 1 animals had significantly more degenerated embryos than Group 2 ewes (2.6±1.2 compared with 0.6±0.3/ewe, respectively). A superovulatory protocol wherein pFSH injections were given at 0800 and 1600h was more effective in terms of inducing multiple ovulations than the protocol with 12-h intervals between consecutive pFSH doses, but it was not associated with an increased production of transferable quality embryos by anestrous ewes.

Potential role for human menopausal gonadotropin in ovine superovulatory regimens during the breeding season

“…and 11.1 transferable embryos per ewe, respectively, which was equal or greater than the response reported by other researchers using FSH (Bartlewski et al, 2017;Bettencourt et al, 2008;Forcada et al, 2011;King et al, 2022;Menchaca et al, 2009;Quan et al, 2011). In cattle, hMG can reduce ovulatory variability among individual animals (Lauria et al, 1982), an observation we failed to detect in sheep.…”

“…In this context, our observations agreed with hMG studies involving cattle and sheep (Lauria et al, 1982; Schiewe et al, 1990; Scudamore et al, 1991; Sugano et al, 2001). FSH and hMG stimulated an average of 10.4 and 11.1 transferable embryos per ewe, respectively, which was equal or greater than the response reported by other researchers using FSH (Bartlewski et al, 2017; Bettencourt et al, 2008; Forcada et al, 2011; King et al, 2022; Menchaca et al, 2009; Quan et al, 2011). In cattle, hMG can reduce ovulatory variability among individual animals (Lauria et al, 1982), an observation we failed to detect in sheep.…”

“…Embryos were recovered from these donor ewes through a midventral laparotomy at day 19 (Table 1) under general anaesthesia induced with xylazine as previously described (Bartlewski et al, 2017). In brief, the reproductive tract was exposed and corpora lutea were identified.…”