Effect of alternative C2 carbon sources on the growth, lipid, and γ-linolenic acid production of spirulina (Arthrospira platensis)

Abstract

The influence of different organic carbon sources (glucose, ethanol, and acetic acid) at different concentrations (0.1, 0.5, and 1.0 g/L for batch and 1.0, 2.0, and 3.0 g/L for fed-batch) were studied in the mixotrophic production (using both light and carbon source) of γ-linolenic acid (GLnA) by spirulina (Arthrospira platensis). The obtained spirulina was analyzed in terms of biomass, lipid, and GLnA production. In the batch media, increasing the concentrations of glucose, ethanol, and acetic acid led to an increase in the biomass, lipid, and GLnA production. However, carbon sources at concentrations greater than 1.0 g/L in fed-batch media appeared to have no significant effects on the above parameters. It was also demonstrated that biomass, lipid, and GLnA production using ethanol and acetic acid could be as good as those achieved with the classic glucose-based culture media.

Effect of <em>Thymus vulgaris</em> and <em>Bunium persicum</em> essential oils on the oxidative stability of virgin olive oil

“…Fatty acid methyl esters were prepared here according to the method described by Golmakani et al (2012). The composition and types of fatty acids in the VOO sample was analyzed using a GC system (SP-3420A, Beijing Beifen-Ruili Analytical Instrument, Beijing, China) which is a device equipped with a split/splitless injector, a flame ionization detector (FID) and a BPX70 capillary column (Bis-cyanopropylsiloxane-silphenylene, 120 m × 0.25 mm internal diameter; 0.25 μm film thickness, SGE Analytical Science, Melbourne, Australia).…”

Oxidative stability of UV irradiated and X-rayed soybean oil incorporated with rose oil

“…To determine the fatty acid composition, fatty acid methyl esters (FAMEs) of SBO were prepared according to the method described by Golmakani et al, (2012). For this purpose, a GC system (SP-3420A, Beijing, China) was equipped with an FID and a BPX-70 fused silica capillary column (30 m × 0.25 mm internal diameter, 0.25 µm film thickness) and was used accordingly.…”

Untitled

“…To determine the fatty acid profile of KO, fatty acid methyl esters (FAMEs) were prepared following the method of Golmakani, Rezaei, Mazidi, and Razavi (2012). Fatty acid profile of KO was analysed using a GC system (SP-3420A, Beijing Beifen-Ruili Analytical Instrument, Beijing, China) equipped with a split/splitless injector, BPX70 capillary column (30 m × 0.25 mm id; 0.25 μm film thickness, SGE Analytical Science, Melbourne, Australia), and a flame ionization detector (FID) according to the method of .…”