Effect of alternative cryopreservation procedures on bull semen

Abstract

Summary:The aim of this study was to assess the use of -152°C ultra freezer for freezing and storing bull semen as an alternative to freezing over the liquid nitrogen vapor and storing in liquid nitrogen in terms of certain post-thaw spermatological parameters. Two ejaculates, one per each bull, were used without pooling. Four different freezing and storing protocols were tested. At 1 week, 2 months and 6 months after cryopreservation, the frozen semen was thawed and the seminal parameters were determined. As a conclusion, this study has confirmed that the use of -152 °C ultra-low freezer for freezing and storing bull semen for 6 months is a viable alternative to liquid nitrogen.Key words: Bull, cryopreservation, semen, storage, ultra-low freezer. Alternatif Dondurma ve Saklama Yöntemlerinin Boğa Sperması Üzerine EtkisiÖzet: Bu çalışmanın amacı, boğa spermasının -152 °C derin dondurucuda dondurulup saklanmasının sıvı azot buharında dondurma ve sıvı azotta saklamaya çözüm sonu kimi spermatolojik parametreler bakımından bir alternatif olup olmayacağının araştırılmasıdır. Bu çalışmada iki adet boğanın birer ejakulatı miks yapılmadan kullanıldı. Araştırmada dört farklı dondurma ve saklama protokolü denendi. Dondurma işleminden bir hafta, iki ve altı aylık periyotlar sonrasında payetler çözdürüldü ve çözüm sonu bazı spermatolojik özellikler değerlendirildi. Sonuç olarak bu çalışma -152°C derin dondurucunun boğa spermasının dondurulması ve altı ay süreyle saklanmasında sıvı azota bir alternatif olarak kullanılabilirliğini ve boğa spermasının dondurulması ve saklanmasında iyi bir ortam sağladığını doğrulamaktadır. Anahtar sözcükler: Boğa, derin dondurucu, dondurma, saklama, sperma.* Aynı isimli doktora tezinden özetlenmiştir.Bull semen can be frozen in pellets, ampoules or straws and then stored in liquid nitrogen (13). In addition to the traditional method for freezing semen in straws, freezers operating with liquid nitrogen are also used (2,3,4,11). However, with the changes in life sciences, liquid nitrogen-based systems have faced with some problems, the most important two being the contamination risk and temperature change up to 90 °C (4,6,7,8,10). Therefore, there is a need to investigate feasible alternatives for semen cryopreservation which can prevent the complications of traditional methods (2). However, there are also other studies confirming that another viable alternative may be the use of ultra-low freezers at -152 °C, not too far from liquid nitrogen temperature at -196 °C (2,3,4,11). It remains to be established mechanical freezers which can provide air phase at -150°C without the need for liquid nitrogen can prevent cross-contamination of germ-plasm over a long period of storage (6,7). The aim of this experiment was to assess the use of an ultra-low freezer at -152 °C for freezing and storage of bull semen for 6 months. Postthawing seminal parameters were evaluated to see whether an ultra-low freezer at -152 °C could be a feasible alternative to cryopreservation in liquid nitrogen.Two Holstein bulls we...

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