Effect of Amphotericin B on Dopachrome Tautomerase Activity and Other Melanogenic Parameters in Cultured B16/F10 Melanoma Cells

Peinado, P.; Martinez-Liarte, J. H.; Solano, Francisco; Lozano, José Antonio

doi:10.1111/j.1600-0749.1992.tb00570.x

Cited by 2 publications

(4 citation statements)

References 11 publications

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“…Concerning dopa oxidase and tyrosine hydroxylase activities, both of them showed an increase after treatment, in a similar way as it happened with the amphotericin B (Johnson and Bagnara, 1990;Peinado et al, 1992), although the percentage of activation obtained was clearly lower than that obtained after the anifungi action for 24 hr. Thus, the stimulation of these tyrosinase activities was always lower It is worth mention that two proteins, tyrosinase and TRP1, encoded by the albino and brown loci, respectively than two-fold.…”

Section: Resultssupporting

confidence: 71%

“…noticeable as soon as 4 hr. It is notable that this behavior was very different from those shown by these activities after treatment and removal of amphotericin B (Peinado et al, 1992).…”

Section: Evolution Of the Effect After Antibiotics Suppressionmentioning

confidence: 67%

“…There have been recent reports on the effect that one of them, the antifungal reagent amphotericin B (hngizone), produced on several melanogenic parameters, like enzymatic activities or melanin content, in cultured B16/F10 malignant melanocytes (Johnson and Bagnara, 1990;Peinado et al, 1992). Through these studies it has been proven that routine concentrations of the reagent (2.5 pg/ ml) caused an activation in dopachrome tautomerase (DCT), dopa oxidase and tyrosine hydroxylase activities, and melanin content in these cells.…”

Section: Introductionmentioning

confidence: 99%

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Effect of Penicillin‐Streptomycin and Other Antibiotics on Melanogenic Parameters in Cultured B16/F10 Melanoma Cells

Martinez-Liarte

Solano

Lozano

1995

Pigment Cell Research

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Penicillin and streptomycin, the most widely used antibiotics in mammalian cell cultures, caused a moderate stimulation in dopa oxidase and tyrosine hydroxylase activities, but a slight inactivation in the dopachrome tautomerase activity of B16/F10 melanoma cells at the routine concentration (100 units/ml penicillin and 100 micrograms/ml streptomycin) used for preventing bacterial growth in cultured animal cells. At these concentrations, tyrosinase activities and melanin content augmented with time during the first 24-48 hr. The opposite effect acted on cell viability. After withdrawal of the antibiotics from the culture medium, the recovery of melanogenic parameters to normal values was fully reached after few hours (around 10), and it was already noticeable as soon as 4 hr after removal. Other antibiotics used in cell culture, like kanamycin, gentamicin, and the antimicotic nystatin, exerted similar low effects at the recommended concentrations, always lower than two-fold and thus lower than those reported for amphotericin B. Taking into account these relatively low effects, and the high risk of contamination of mammalian cells culture without antibiotics, penicillin and streptomycin may still be routinely used in experiments leading to explore the melanogenic activity of malignant melanocytes in culture, unless very precise studies and strict conditions were needed.

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Section: Resultssupporting

confidence: 71%

“…noticeable as soon as 4 hr. It is notable that this behavior was very different from those shown by these activities after treatment and removal of amphotericin B (Peinado et al, 1992).…”

Section: Evolution Of the Effect After Antibiotics Suppressionmentioning

confidence: 67%

Section: Introductionmentioning

confidence: 99%

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Effect of Penicillin‐Streptomycin and Other Antibiotics on Melanogenic Parameters in Cultured B16/F10 Melanoma Cells

Martinez-Liarte

Solano

Lozano

1995

Pigment Cell Research

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“…Indeed, melanin deficient (mel − ) strains L. prolificans generated by UV mutagenesis [ 43 ] were shown to be as resistant to amphotericin B, nystatin and azoles as the wild-type parent. Despite this, amphotericin B has been shown to increase the melanin content of melanoma cells [ 54 ]. An alternative explanation for the increased growth of mutants Δ Lppks1 :: hph and Δ Lpscd1 :: hph might be compensation for DHN-melanin loss through alternative pathways of melanin biosynthesis.…”

Section: Discussionmentioning

confidence: 99%

Targeted Disruption of Melanin Biosynthesis Genes in the Human Pathogenic Fungus Lomentospora prolificans and Its Consequences for Pathogen Survival

Al-laaeiby

Kershaw

Penn

et al. 2016

IJMS

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The dematiaceous (melanised) fungus Lomentospora (Scedosporium) prolificans is a life-threatening opportunistic pathogen of immunocompromised humans, resistant to anti-fungal drugs. Melanin has been shown to protect human pathogenic fungi against antifungal drugs, oxidative killing and environmental stresses. To determine the protective role of melanin in L. prolificans to oxidative killing (H2O2), UV radiation and the polyene anti-fungal drug amphotericin B, targeted gene disruption was used to generate mutants of the pathogen lacking the dihydroxynaphthalene (DHN)-melanin biosynthetic enzymes polyketide synthase (PKS1), tetrahydroxynapthalene reductase (4HNR) and scytalone dehydratase (SCD1). Infectious propagules (spores) of the wild-type strain 3.1 were black/brown, whereas spores of the PKS-deficient mutant ΔLppks1::hph were white. Complementation of the albino mutant ΔLppks1::hph restored the black-brown spore pigmentation, while the 4HNR-deficient mutant ΔLp4hnr::hph and SCD-deficient mutant ΔLpscd1::hph both produced orange-yellow spores. The mutants ΔLppks1::hph and ΔLp4hnr::hph showed significant reductions in spore survival following H2O2 treatment, while spores of ΔLpscd1::hph and the ΔLppks1::hph complemented strain ΔLppks1::hph:PKS showed spore survivals similar to strain 3.1. Spores of the mutants ΔLp4hnr::hph and ΔLpscd1::hph and complemented strain ΔLppks1::hph:PKS showed spore survivals similar to 3.1 following exposure to UV radiation, but survival of ΔLppks1::hph spores was significantly reduced compared to the wild-type strain. Strain 3.1 and mutants ΔLp4hnr::hph and ΔLppks1::hph:PKS were resistant to amphotericin B while, paradoxically, the PKS1- and SCD1-deficient mutants showed significant increases in growth in the presence of the antifungal drug. Taken together, these results show that while melanin plays a protective role in the survival of the pathogen to oxidative killing and UV radiation, melanin does not contribute to its resistance to amphotericin B.

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Effect of Amphotericin B on Dopachrome Tautomerase Activity and Other Melanogenic Parameters in Cultured B16/F10 Melanoma Cells

Cited by 2 publications

References 11 publications

Effect of Penicillin‐Streptomycin and Other Antibiotics on Melanogenic Parameters in Cultured B16/F10 Melanoma Cells

Effect of Penicillin‐Streptomycin and Other Antibiotics on Melanogenic Parameters in Cultured B16/F10 Melanoma Cells

Targeted Disruption of Melanin Biosynthesis Genes in the Human Pathogenic Fungus Lomentospora prolificans and Its Consequences for Pathogen Survival

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