Effect of Anatase TiO<sub>2</sub> Nanoparticles on the Growth of RSC-364 Rat Synovial Cell

Wang, Jiangxue; Ma, Jianglin; Dong, Linmeng; Hou, Ying; Jia, Xin; Niu, Xufeng; Fan, Yubo

doi:10.1166/jnn.2013.7145

Cited by 22 publications

(18 citation statements)

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“…While the common approach in studying effects of TiO 2 nanoparticles on cells is to measure cell proliferation decrease after exposure to high nanoparticles concentration [ 53 ] we decided to focus on the concentrations that do not affect cell proliferation. Our results allow a deeper understanding of cell membrane alterations induced by TiO 2 nanoparticles, in the context of how changes in membrane mechanics can alter cell-bacteria interaction.…”

Section: Discussionmentioning

confidence: 99%

Exposure to TiO2 nanoparticles increases Staphylococcus aureus infection of HeLa cells

Wei

Ou-Yang

et al. 2016

J Nanobiotechnol

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BackgroundTitanium dioxide (TiO2) is one of the most common nanoparticles found in industry ranging from food additives to energy generation. Approximately four million tons of TiO2 particles are produced worldwide each year with approximately 3000 tons being produced in nanoparticulate form, hence exposure to these particles is almost certain.ResultsEven though TiO2 is also used as an anti-bacterial agent in combination with UV, we have found that, in the absence of UV, exposure of HeLa cells to TiO2 nanoparticles significantly increased their risk of bacterial invasion. HeLa cells cultured with 0.1 mg/ml rutile and anatase TiO2 nanoparticles for 24 h prior to exposure to bacteria had 350 and 250 % respectively more bacteria per cell. The increase was attributed to bacterial polysaccharides absorption on TiO2 NPs, increased extracellular LDH, and changes in the mechanical response of the cell membrane. On the other hand, macrophages exposed to TiO2 particles ingested 40 % fewer bacteria, further increasing the risk of infection.ConclusionsIn combination, these two factors raise serious concerns regarding the impact of exposure to TiO2 nanoparticles on the ability of organisms to resist bacterial infection.Electronic supplementary materialThe online version of this article (doi:10.1186/s12951-016-0184-y) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Exposure to TiO2 nanoparticles increases Staphylococcus aureus infection of HeLa cells

Wei

Ou-Yang

et al. 2016

J Nanobiotechnol

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“…Cell culture. The rat FLS cell line, RSC-364 (21,22), used in the present study was a generous gift from Dr Jingxiang Huang (Chinese People's Liberation Army General Hospital, Bejing, China). Cells were cultured in DMEM containing 10% fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc.) supplemented with 100 U/ml penicillin and 100 µg/ml streptomycin (Sigma-Aldrich) at 37˚C in a humidified atmosphere of 5% CO 2 in air.…”

Section: Methodsmentioning

confidence: 99%

Chlorogenic acid induces apoptosis to inhibit inflammatory proliferation of IL-6-induced fibroblast-like synoviocytes through modulating the activation of JAK/STAT and NF-κB signaling pathways

Lou

Zhou

Liu

et al. 2016

Experimental and Therapeutic Medicine

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Chlorogenic acid (CGA) is the primary constituent of Caulis , a Chinese herb used for the treatment of rheumatoid arthritis (RA). The present study aimed to investigate whether CGA was able to inhibit the proliferation of the fibroblast-like synoviocyte cell line (RSC-364), stimulated by interleukin (IL)-6, through inducing apoptosis. Following incubation with IL-6 or IL-6 and CGA, the cellular proliferation of RSC-364 cells was detected by MTT assay. The ratio of apoptosed cells were detected by flow cytometry. Western blot analysis was performed to observe protein expression levels of key molecules involved in the Janus-activated kinase/signal transducer and activator of transcription 3 (JAK/STAT) signaling pathway [phosphorylated (p)-STAT3, JAK1 and gp130] and the nuclear factor κB (NF-κB) signaling pathway [phosphorylated (p)-inhibitor of κB kinase subunit α/β and NF-κB p50). It was revealed that CGA was able to inhibit the inflammatory proliferation of RSC-364 cells mediated by IL-6 through inducing apoptosis. CGA was also able to suppress the expression levels of key molecules in the JAK/STAT and NF-κB signaling pathways, and inhibit the activation of these signaling pathways in the inflammatory response through IL-6-mediated signaling, thereby resulting in the inhibition of the inflammatory proliferation of synoviocytes. The present results indicated that CGA may have potential as a novel therapeutic agent for inhibiting inflammatory hyperplasia of the synovium through inducing synoviocyte apoptosis in patients with RA.

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“…Wang et al 38 noticed that TiO 2 NPs induce oxidative stress in a rat synovial cell line (RSC-364) by increasing the generation of free radicals. It has been previously demonstrated that metal, half-metal, and metal-oxide NPs induce production of ROS in different cell lines.…”

Section: Titanium Dioxide Nanoparticles Induce Generation Of the Supementioning

confidence: 99%

Titanium dioxide nanoparticles enhance production of superoxide anion and alter the antioxidant system in human osteoblast cells

Niska¹,

Pyszka²,

Tukaj³

et al. 2015

IJN

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Titanium dioxide (TiO 2 ) nanoparticles (NPs) are manufactured worldwide for a variety of engineering and bioengineering applications. TiO 2 NPs are frequently used as a material for orthopedic implants. However, to the best of our knowledge, the biocompatibility of TiO 2 NPs and their effects on osteoblast cells, which are responsible for the growth and remodeling of the human skeleton, have not been thoroughly investigated. In the research reported here, we studied the effects of exposing hFOB 1.19 human osteoblast cells to TiO 2 NPs (5–15 nm) for 24 and 48 hours. Cell viability, alkaline phosphatase (ALP) activity, cellular uptake of NPs, cell morphology, superoxide anion (O 2 •−2 ) generation, superoxide dismutase (SOD) activity and protein level, sirtuin 3 (SIR3) protein level, correlation between manganese (Mn) SOD and SIR, total antioxidant capacity, and malondialdehyde were measured following exposure of hFOB 1.19 cells to TiO 2 NPs. Exposure of hFOB 1.19 cells to TiO 2 NPs resulted in: (1) cellular uptake of NPs; (2) increased cytotoxicity and cell death in a time- and concentration-dependent manner; (3) ultrastructure changes; (4) decreased SOD and ALP activity; (5) decreased protein levels of SOD1, SOD2, and SIR3; (6) decreased total antioxidant capacity; (7) increased O 2 •− generation; and (8) enhanced lipid peroxidation (malondialdehyde level). The linear relationship between the protein level of MnSOD and SIR3 and between O 2 •− content and SIR3 protein level was observed. Importantly, the cytotoxic effects of TiO 2 NPs were attenuated by the pretreatment of hFOB 1.19 cells with SOD, indicating the significant role of O 2 •− in the cell damage and death observed. Thus, decreased expression of SOD leading to increased oxidizing stress may underlie the nanotoxic effects of TiO 2 NPs on human osteoblasts.

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Effect of Anatase TiO₂ Nanoparticles on the Growth of RSC-364 Rat Synovial Cell

Cited by 22 publications

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Exposure to TiO2 nanoparticles increases Staphylococcus aureus infection of HeLa cells

Exposure to TiO2 nanoparticles increases Staphylococcus aureus infection of HeLa cells

Chlorogenic acid induces apoptosis to inhibit inflammatory proliferation of IL-6-induced fibroblast-like synoviocytes through modulating the activation of JAK/STAT and NF-κB signaling pathways

Titanium dioxide nanoparticles enhance production of superoxide anion and alter the antioxidant system in human osteoblast cells

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Effect of Anatase TiO2 Nanoparticles on the Growth of RSC-364 Rat Synovial Cell

Cited by 22 publications

References 0 publications

Exposure to TiO2 nanoparticles increases Staphylococcus aureus infection of HeLa cells

Exposure to TiO2 nanoparticles increases Staphylococcus aureus infection of HeLa cells

Chlorogenic acid induces apoptosis to inhibit inflammatory proliferation of IL-6-induced fibroblast-like synoviocytes through modulating the activation of JAK/STAT and NF-κB signaling pathways

Titanium dioxide nanoparticles enhance production of superoxide anion and alter the antioxidant system in human osteoblast cells

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Effect of Anatase TiO₂ Nanoparticles on the Growth of RSC-364 Rat Synovial Cell