Effect of Bacillus subtilis (DSM 17299) on performance, digestibility, intestine morphology, and pH in broiler chickens

Reis, Matheus de Paula; Fassani, Édison José; Júnior, Antônio Amândio Pinto Garcia; Rodrigues, Paulo Borges; Bertechini, A. G.; Barrett, Nathaniel W.; Persia, Michael E.; Schmidt, Carl J.

doi:10.3382/japr/pfx032

Cited by 38 publications

(32 citation statements)

References 49 publications

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“…In this study, adding B. subtilis or B. licheniformis to broilers' diet improved apparent digestibility of protein and ME compared to the control group. In agreement with our findings, a recent study has shown that supplementing B. subtilis improved total tract digestibility of DM, CP, and ME in broiler chickens (Reis et al 2017). As mentioned before, the ileal pH of broilers fed with probiotics was lower than control birds which can help the absorption of protein and minerals like Copper, Ca, Iron, Manganese, and Magnesium (Raghuwanshi et al 2015).…”

Section: Apparent Ileal and Total Tract Nutrient Digestibilitysupporting

confidence: 92%

Comparison of two probiotic preparations on growth performance, intestinal microbiota, nutrient digestibility and cytokine gene expression in broiler chickens

Zaghari

Sarani

Hajati

2020

Journal of Applied Animal Research

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This study was done to evaluate the effects of two dietary probiotic preparations (Bacillus subtilis and Bacillus licheniformis) on growth performance, intestinal microbiota, nutrient digestibility and cytokine gene expression in broiler chickens. A total of 180 male broiler chicks (one-day-old Ross 308 strain, average initial body weight = 40.05 ± 0.12) were used in a completely randomized design (CRD) composed of 3 treatments and 6 replicates. Experimental diets included: (1) basal diet (without additive), (2) basal diet plus 0.5 g/kg diet B. subtilis preparation (1 × 10 9 CFU/g), (3) basal diet plus 0.5 g/kg diet B. licheniformis preparation (1 × 10 9 CFU/g). The results showed that supplementation of B. licheniformis improved (P < 0.05) broilers' body weight gain (BWG), feed conversion ratio (FCR), and production efficiency factor (PEF). Adding B. licheniformis caused the lowest (P < 0.01) feed cost per kilogram weight gain and the highest (P < 0.05) return of investment (ROI). Probiotic treatments significantly decreased (P < 0.01) ileal pH of the broilers. Probiotic treatments improved (P < 0.01) apparent metabolizable energy (AME) and total tract digestibility of protein. It was concluded that although both probiotic bacteria improved AME and total tract protein digestibility, B. licheniformis was superior to B. subtilis in improving broiler chickens' BWG (2580.70 vs. 2427.45 g) and their PEF (418.95 vs. 374.49).

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Section: Apparent Ileal and Total Tract Nutrient Digestibilitysupporting

confidence: 92%

Comparison of two probiotic preparations on growth performance, intestinal microbiota, nutrient digestibility and cytokine gene expression in broiler chickens

Zaghari

Sarani

Hajati

2020

Journal of Applied Animal Research

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“…The beneficial effects of dietary Bacillus spp. probiotic supplementation on FCR and increased BW of broilers are well documented in many studies (Opalinski et al, 2007;Melegy et al, 2011;Yang et al, 2016;Reis et al, 2017). Spore-forming Bacillus spp.…”

Section: Discussionmentioning

confidence: 91%

“…These results are in agreement with those of Hatab et al (2016), who reported that dietary supplementation with Bacillus spp. probiotics (B. tequilensis K03 strain and B. subtilis DSM 17299) rose carcass and relative organ weights due to increase of cell growth and turnover, while other researchers reported that (Afsharmanesh et al, 2014;Park et al, 2014;Reis et al, 2017;Shokryazdan et al, 2017), using the same or different probiotic species did not affect the relative organ weights of broilers. The reason for these contradictions may be due to differences in conditions of chickens, methods of administration, viability and concentrations of used bacteria, as well as the strain sources (Shokryazdan et al, 2017).…”

Section: Discussionmentioning

confidence: 92%

Effects of Indigenous Spore-Forming Probiotic as Feed Supplement on Performance and Safety in Broilers

Hosseini

Modarressi

Mousavi

et al. 2020

J Hellenic Vet Med Soc

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Probiotics colonize the intestine of animals and birds and provide useful effects on their performance and immune status. This study describes a high throughput screening and characterization of spore-forming bacteria from Iranian poultry farms with the aim to identify potential probiotic native Bacillus spp. and determine its effects on growth performance, hemato-biochemical parameters, immunity, intestinal microflora, morphology and MUC2 gene expression of broiler chickens. A total of 300 one-day-old female Ross 308 broilers (42.6 ± 0.6 g) were used in a 6-wk study. Broilers were randomly allotted to 1 of 3 dietary treatments consisting of 4 replicate cages with 25 broilers each: 1-Control (Corn-soy-based diet: C), 2-C + 200 g/ton of the GalliPro® (Bacillus subtilis DSM 17299, 4×10 9 CFU/g, as positive control group: PC), 3-C + 200 g/ton of the native probiotic (B. tequilensis K03, 4×10 9 CFU/g: NP) identified in this study. During the experiment parameters were measured weekly. The results revealed that birds of the NP and PC groups exhibited improved feed conversion ratio (FCR) and increased body weight (BW), carcass and breast meat yield compared with the birds of the C group (P<0.05). Also, lymphocytes level, antibody titers against Newcastle diseases virus (NDV) and infectious bronchitis virus (IBV) of vaccinated birds were increased, while serum triglycerides, total cholesterol levels and abdominal fat of birds fed NP and PC were decreased compared to birds of the C group (P<0.05). The villus height, the relative expression of MUC2 gene and Bacillus spp. populations were increased, while E. coli was significantly decreased in the ileum content of treated groups (P<0.05). These results indicate that the identified native B.tequilensis K03 strain can improve immunity and broiler performance by modifying intestinal microflora and morphology. Studied native probiotic Bacillus tequilensis K03 has useful effects on health status and it can be used as poultry feed supplement.

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“…subtilis DSM 32315 decreased the relative weight of ileum in broilers, which was similar to the study of Reis et al . 20 who reported an improved growth performance concomitant with a lessened relative weight of duodenum of broilers after B . subtilis (DSM 17299) addition.…”

Section: Discussionmentioning

confidence: 99%

Supplemental Bacillus subtilis DSM 32315 manipulates intestinal structure and microbial composition in broiler chickens

Wang

Zhang

et al. 2018

Sci Rep

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Knowledge about the modulation of gut microbiota improves our understanding of the underlying mechanism by which probiotic treatment benefits the chickens. This study examined the effects of Bacillus subtilis DSM 32315 on intestinal structure and microbial composition in broilers. Broiler chicks were fed basal diets without or with B. subtilis supplementation (1.0 × 109 spores/kg of diet). Supplemental B. subtilis increased average body weight and average daily gain, as well as elevated villus height and villus height to crypt depth ratio of ileum in broilers. Multi-dimension analysis showed a certain degree of separation between the cecal microbiota from treatment and control groups. Increased Firmicutes abundance and reduced Bacteroidetes abundance in cecum were observed responded to B. subtilis addition, which also increased the abundances of Christensenellaceae and Caulobacteraceae, and simultaneously decreased the abundances of potentially harmful bacteria such as Vampirovibrio, Escherichia/Shigella and Parabacteroides. Network analysis signified that B. subtilis addition improved the interaction pattern within cecal microbiota of broilers, however, it exerted little influence on the metabolic pathways of cecal microbiota by comparison of the functional prediction of metagenomes. In conclusion, supplemental B. subtilis DSM 32315 improved growth performance and intestinal structure of broilers, which could be at least partially responsible by the manipulation of cecal microbial composition.

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Effect of Bacillus subtilis (DSM 17299) on performance, digestibility, intestine morphology, and pH in broiler chickens

Cited by 38 publications

References 49 publications

Comparison of two probiotic preparations on growth performance, intestinal microbiota, nutrient digestibility and cytokine gene expression in broiler chickens

Comparison of two probiotic preparations on growth performance, intestinal microbiota, nutrient digestibility and cytokine gene expression in broiler chickens

Effects of Indigenous Spore-Forming Probiotic as Feed Supplement on Performance and Safety in Broilers

Supplemental Bacillus subtilis DSM 32315 manipulates intestinal structure and microbial composition in broiler chickens

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