Effect of Bacillus subtilis on the rumen microbial community and its components exhibiting high correlation coefficients with the host nutrition, growth, and development

Ушакова, Н. А.; Некрасов, Р.В.; Мелешко, Н. А.; Laptev, G. Yu.; Ильина, Л. А.; Козлова, А. А.; Nifatov, A. V.

doi:10.1134/s0026261713040127

Cited by 20 publications

(11 citation statements)

References 12 publications

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“…Additionally, developing calves fed Bacillus subtilis exhibited a fraction of the amount of bacteria from the family Bifidobacteriaceae as well as an increased amount of microbes from Lactobacillaceae compared to the control. As well, the average daily increase in body mass of the experimental groups of calves, with low amount of ruminal Bifidobacteriaceae, was significantly higher (p ≤ 0.05) than that of the control [27]. When correlated with our results, this may be due to the negative effect of Bifidobacteria on size.…”

Section: Discussionsupporting

confidence: 86%

Phenotypic effects of probiotics on Xenopus laevis development

Oosbree¹,

Kiesow

2017

J Stud Res

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Probiotics supplementation, such as Lactobacillus acidophilus and Bifidobacteria, has become increasingly popular as a means to naturally treat minor ailments and attain overall better health. This research sought to determine whether probiotics L. acidophilus and Bifidobacteria as well as a combination (Lactobacilli and Bifidobacteria) had an impact on the development of Xenopus laevis (African clawed frog) embryos. We used several control and experimental groups per probiotic species. For experimental groups, each probiotic species (or combination) was isolated and dissolved in dechlorinated distilled water to create a 0.0025% and 0.025% solution. Embryos were measured/observed to determine presence of abnormalities; collect intraocular, gut, and head measurements; and examine behavior. After organogenesis, we euthanized embryos to examine the presence of specific species of bacteria using EnteroPluri tubes. All groups showed similar mortality, morphology, and activity (P > 0.05), except for those treated with Bifidobacteria (P ? 0.05), and in all instances, activity and stage of development were positively correlated (r = 0.38 to 0.69). Gut bacterial composition was similar between Bifidobacteria and control groups, but gut compositions were different among L. acidophilus and combination (Lactobacilli and Bifidobacteria) groups. All data combine to indicate that Bifidobacteria should be limited or avoided as it contributes to smaller overall embryo size and higher activity levels, while L. acidophilus consumed at a 0.0025% concentration (recommended dosage) is the best option as a probiotic supplement as it provides the benefits associated with this probiotic without adversely impacting the embryo. We suggest additional research to examine the positive influence of L. acidophilus, as these data suggest its usefulness as a probiotic.

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Section: Discussionsupporting

confidence: 86%

Phenotypic effects of probiotics on Xenopus laevis development

Oosbree¹,

Kiesow

2017

J Stud Res

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“…Another explanation would be that BS could suppress E. coli while promoting anaerobic intestinal probiotics growth in symbiosis with them ( Stanley et al., 2014 ). Ushakova et al. (2013) found that BS can secrete pathogen-suppressive substances that have bacteriostatic action on common pathogens such as Staphylococcus aureus and E. coli which were highly sensitive to sample concentrate, and the bacteriostatic effect is equivalent to normal antibiotics.…”

Section: Discussionmentioning

confidence: 99%

Effect of Bacillus subtilis on growth performance, bone mineralization, and bacterial population of broilers fed with different protein sources

et al. 2020

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The objective of this study was to investigate the impacts of Bacillus subtilis ( BS ), ATCC 6051a strain, as a probiotic bacterium in broiler diets based of 2 protein sources (soybean meal [ SBM ] and cowpea seeds [ CWP ]), on growth performance ( GP ), carcass traits, bone mineralization, and microflora population (0 to 42 d age). The SBM and CWP starter, grower, and finisher diets were tested in the presence or absence of BS (5.0 × 10 11 CFU spores g −1 feed) in a 2 × 2 factorial arrangement of treatments in a completely randomized design. Broilers were randomly assigned to 4 dietary treatments with 6 replicate pens per treatment (20 chicks per pen). The results showed that broilers fed CWP had comparable GP (body weight gain, feed intake, and feed conversion ratio) to the birds fed the SBM diet. Carcass, breast and legs' yield, organ size (i.e., gizzard, liver, pancreas, small intestine, cecum), and bone development were not affected by the protein source. The addition of BS in both types of diet improved BWG ( P < 0.001) and feed efficiency, especially in the grower and finisher period ( P = 0.047; P = 0.043 , respectively). In addition, BS significantly decreased abdominal fat ( P = 0.026) and cecum weight ( P = 0.034) and increased tibia bone P concentration ( P = 0.015). Furthermore, BS decrease cecal pH ( P = 0.010) and reduced Escherichia coli and Staphylococcus spp. from cecum and excreta broilers ( P < 0.001; P < 0.0001 , respectively). It is concluded that the BS significantly improved the GP of broilers and can beneficially affect the gut and excreta bacterial community in both SBM and CWP diets.

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“…Perhaps not surprisingly, the lactic acid bacteria Streptococcus and Lactobacillus tolerated aciduric challenge well in the cow rumen ( Petri et al, 2013 ). Representatives of the genera Bacillus and Lactobacillus produce organic acids, some of which may endow probiotic effects ( Qiao et al, 2010 ; Seo et al, 2013 ; Ushakova et al, 2013 ). The genus Paenibacillus has been noted for its hemicellulose-degrading activity ( Kala et al, 2017 ), while the genus Sharpea tends to form and metabolize lactate an thereby increased its relative abundance in low methane yield sheep rumen ( Kamke et al, 2016 ).…”

Section: Discussionmentioning

confidence: 99%

The Planktonic Core Microbiome and Core Functions in the Cattle Rumen by Next Generation Sequencing

Wirth

Kádár²,

Kakuk

et al. 2018

Front. Microbiol.

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The cow rumen harbors a great variety of diverse microbes, which form a complex, organized community. Understanding the behavior of this multifarious network is crucial in improving ruminant nutrient use efficiency. The aim of this study was to expand our knowledge by examining 10 Holstein dairy cow rumen fluid fraction whole metagenome and transcriptome datasets. DNA and mRNA sequence data, generated by Ion Torrent, was subjected to quality control and filtering before analysis for core elements. The taxonomic core microbiome consisted of 48 genera belonging to Bacteria (47) and Archaea (1). The genus Prevotella predominated the planktonic core community. Core functional groups were identified using co-occurrence analysis and resulted in 587 genes, from which 62 could be assigned to metabolic functions. Although this was a minimal functional core, it revealed key enzymes participating in various metabolic processes. A diverse and rich collection of enzymes involved in carbohydrate metabolism and other functions were identified. Transcripts coding for enzymes active in methanogenesis made up 1% of the core functions. The genera associated with the core enzyme functions were also identified. Linking genera to functions showed that the main metabolic pathways are primarily provided by Bacteria and several genera may serve as a “back-up” team for the central functions. The key actors in most essential metabolic routes belong to the genus Prevotella. Confirming earlier studies, the genus Methanobrevibacter carries out the overwhelming majority of rumen methanogenesis and therefore methane emission mitigation seems conceivable via targeting the hydrogenotrophic methanogenesis.

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Effect of Bacillus subtilis on the rumen microbial community and its components exhibiting high correlation coefficients with the host nutrition, growth, and development

Cited by 20 publications

References 12 publications

Phenotypic effects of probiotics on Xenopus laevis development

Phenotypic effects of probiotics on Xenopus laevis development

Effect of Bacillus subtilis on growth performance, bone mineralization, and bacterial population of broilers fed with different protein sources

The Planktonic Core Microbiome and Core Functions in the Cattle Rumen by Next Generation Sequencing

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