Effect of Bacillus thuringiensis on the Biological Parameters and Phytophagy of Podisus nigrispinus (Hemiptera: Pentatomidae)

Abstract

Effect of Bacillus thuringiensis on the Biological Parameters and Phytophagy of Podisus nigrispinus (Hemiptera: Pentatomidae). -Entomol Gener 34 (4): 313-321; Stuttgart 2013-11. ---[Note]The effect of Bacillus thuringiensis Berliner 1911 var aizawai strain GC-91 (Bta) on the biological parameters and phytophagy of Podisus nigrispinus (Dallas 1851) (Hemiptera: Pentatomidae) were evaluated using the follow treatments: a) provision of deionized water and prey Plutella xylostella (Linnaeus 1758) (Lepidoptera: Plutellidae); b) provision of only a solution containing Bta; and c) provision of prey and the solution containing Bta. To evaluate the phytophagy of the predator, leaves of Brassica oleraceae var acephala Linnaeus cv Manteiga da Geórgia were provided and replaced every two days, and subsequently stained by immersion in 1% acid fuchsin. Staining enabled the visualization of the feeding sheath, which allowed for the quantifi cation of punctures infl icted by P nigrispinus. The phytophagy, reproductive capacity and biological cycle in P nigrispinus were negatively affected by the presence of Bta; however, its predatory capacity was not altered.

Interactions of Bacillus thuringiensis bioinsecticides and the predatory stink bug Podisus nigrispinus to control Plutella xylostella

“…To observe the feeding sheaths, each leaf was immersed in 1% fuchsine acid for 12 h (Dibelli et al. ). Then leaves were washed with water, and the number of feeding sheaths was counted using a stereomicroscope (Leica ® M80, Leica Microsystems, Wetzlar, Germany).…”

“…The kale leaves that were removed from the containers every two days and replaced by new ones were used to verify the phytophagy of the predators on the leaves. To observe the feeding sheaths, each leaf was immersed in 1% fuchsine acid for 12 h (Dibelli et al 2013). Then leaves were washed with water, and the number of feeding sheaths was counted using a stereomicroscope (Leica â M80, Leica Microsystems, Wetzlar, Germany).…”

“…; Dibelli et al. ). Therefore, this is the first study to assess the behaviour of P. nigrispinus in contact with Bt‐bioinsecticides only on a plant sprayed.…”

“…Previous studies that have evaluated the interaction between P. nigrispinus and Bt-bioinsecticides have observed the biology of the predator in two situations: the predators consumed only larvae of P. xylostella contaminated with B. thuringiensis, or the predators consumed only bacterial suspension as the source of water (Carvalho et al 2012;Dibelli et al 2013). Therefore, this is the first study to assess the behaviour of P. nigrispinus in contact with Bt-bioinsecticides only on a plant sprayed.…”

Dissecting the Environmental Consequences of Bacillus thuringiensis Application for Natural Ecosystems

“…For predatory bugs, the data on the Bt toxicity differ depending on the experimental setup and the Bt strains involved. For Podisus nigrispinus , the exposure to the Bt var aizawai strain GC-91 contained in the prey and solution negatively affected the phytophagy, reproductive capacity, and biological cycle [ 132 ]. On the contrary, when fed with Plutella xylostella larvae treated with the Bt var.…”