Effect of bee pollen extract on lead-induced toxicity in rat testis

Bouazza, Sofiane; Demmouche, Abbassia; Toumi-Benali, Fawzia; Zouba, M; Bahri, M R; Agher, N.; Merakchi, Nacera; Ahmar, Mama El

doi:10.38150/sajeb.8(3).p91-102

Cited by 5 publications

(3 citation statements)

References 53 publications

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“…The results of the present study are in line with the results of the above studies. In another study, Bouazza et al (2018) stated that lead acetate created empty spaces between germ cells. Aftab and Abdul Rauf, (2014) attributed the vacancy between the germ cells to the Sertoli cell damage, which is caused by genotoxic and antiproliferative effects of compounds such as lead acetate.…”

Section: Discussionmentioning

confidence: 99%

“…BaSalamah et al (2018) in their study reported atrophy of the seminiferous tubules and destruction of germ cells and sertoli cells in the testis while exposed to rat lead acetate. In another study, Bouazza et al (2018) stated that lead acetate reduced the height of the germ epithelium and created empty spaces between the germ cells. Dorostghoal et al (2011) also reported a significant dose-dependent decrease in the height of the germinal epithelium during the neonatal, pre-pubertal, and post-pubertal periods of rats exposed to lead acetate.…”

Section: Groupsmentioning

confidence: 99%

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Impact of Maternal Exposure to Lead Acetate before Pregnancy Through Lactation Period on the Testicular histomorphologic Indices of Male Offspring of Wistar Rats, A Stereological Study

2023

JJBS

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Exposure to heavy metals in the products of various industries and the environment is a permanent threat to humans. Much research had shown the adverse effects of lead acetate even at low doses. The present study was conducted to investigate the effect of lead acetate on testicular histomorphologic indices of male offspring of female Wistar rats that were exposed to lead during pre-pregnancy, pregnancy and lactation periods. After adaption, rats were divided into different cages for mating (1 male to 2 females). After diagnosing pregnancy, rats were divided into three groups: pregnancy, lactation, and pregnancy until the end of lactation (PL). During the mentioned periods, 0.2% lead acetate plus 500 μl of glacial acetic acid were available in drinking water. Female rats belonging to the pre-pregnancy group had access to the mentioned compound for 3 weeks before mating. Also, females belonging to the PrePregnancy to the end of Lactation. had access to the mentioned compound for 3 weeks before pregnancy to the end of lactation. Offspring of control and sham groups also had access to drinking water and 500 μl of glacial acetic acid in drinking water, for survey of deposition of lead acetae, from the beginning to the end of the experimental period (63 days), respectively. At the end of the experiment (postnatal day: 63), adults were sacrificed and their left testicles were fixed in 10% formalin. In order to perform morphometric studies, testicular tissue was randomly divided into equal sections, and for hematoxylin-eosin staining routine tissue preparation steps were performed. Finally, the prepared slides were used for stereological examinations. The results showed that exposure to 0.2% lead acetate had significant reduction on the volumetric density of the seminiferous tubules, the volumetric density of the epithelium of the tubules, the volumetric density of the interstitial tissue and the height of the germinal epithelium of the tubules of experimental groups compared to the control and sham groups. It should be noted that exposure to 0.2% lead acetate, despite the decreasing effects, did not cause a significant change in the length of the tubules and the epithelial surface area of these tubules. We conclude that lead, in the amount of 0.2%, has different effects on the tissue structure of testes of male offspring. Therefore, due to the increasing development of various technologies and industries that are sources of production of this harmful compound, the need to protect the mother and fetus against its adverse effects are felt more than ever.

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Section: Discussionmentioning

confidence: 99%

Section: Groupsmentioning

confidence: 99%

Impact of Maternal Exposure to Lead Acetate before Pregnancy Through Lactation Period on the Testicular histomorphologic Indices of Male Offspring of Wistar Rats, A Stereological Study

2023

JJBS

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“…3,4 Many studies revealed that lead acetate could cause infertility in male. 5 A study revealed that giving 0.1% lead acetate to rats had reduced testis weight, seminiferous tubulous diameter, epididymal sperm count, and serum testosterone level. 6 study revealed that 20 mg/kg BW lead acetate could caused the decrease of testis weight and diameter of tubulous seminiferous in male mice testis.…”

Section: Introductionmentioning

confidence: 99%

Protective Effect of Lemongrass Extract ( Cymbopogon nardus) on Spermatozoa Cells Induced by Lead Acetate

Setiyowati

Solekha

Irawan

et al. 2022

BHSJ

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Introduction: Male infertility can be caused by many factors. One of which is environmental pollution such as lead acetate. Lead acetate exposure can increase Reactive Oxygen Species (ROS), affecting spermatozoa quality. Natural antioxidants and flavonoid on Lemongrass (C. nardus) have a great potential for protecting the male reproductive system. This study aimed to analyze the effect of giving various dosages of C. nardus extract on the sperm quality of mice. Methods: The Balb/C male mice were divided into five equal groups. The negative control group was injected with 0.01 ml Na-CMC 0.5% within 40 days, and the positive control group was injected with 0.01 ml lead acetate 7 mg/kg BW within 5 days, the treatment groups were injected by 0.01 ml lead acetate 7 mg/kg body weight within 5 days and continuously injected by 0.01 ml of C. nardus extract with various dosage 25 mg/kg BW, 50 mg/kg BW, and 100 mg/kg BW within 35 days. At the end of the experiment, mice were sacrificed, and sperm suspensions were collected from cauda epididymal to measure the morphology, concentration, and motility.Results: The results showed that giving C. nardus extract could repair morphology, concentration, and motility of spermatozoa with significantly different (p<0.05). The 25 mg/kg BW dose has a good protective effect. Conclusion: The C. nardus extract can repair the decreasing sperm quality caused by lead acetate exposure.

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Protective effect of bee pollen in acute kidney injury, proteinuria, and crystalluria induced by ethylene glycol ingestion in rats

Elghouizi

Al-Waili

Elmenyiy

et al. 2022

Sci Rep

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Oxidative stress plays a role in hyperoxaluria-induced kidney injury and crystallization. Bee pollen is a hive product with a high content of antioxidants. The antioxidant content and protective effect of bee pollen extract (BPE) against ethylene glycol (EG) induced crystalluria, and acute kidney injury (AKI) were investigated. The effect of BPE on the EG-induced liver injury and proteinuria was also examined. Ten groups of male Wister rats were treated daily with vehicle, cystone, BPE (100, 250, and 500 mg/kg b.wt.), and group 6–9 treated with EG, EG + BPE (100, 250, and 500 mg/kg b.wt.) and group 10 EG + cystone. The dose of EG was 0.75% v/v, and the dose of cystone was 500 mg/kg b.wt. On day 30, blood and urine samples were collected for analysis. Kidneys were removed for histopathological study. The antioxidant activity of BPE was assessed, and its total phenols and flavonoids were determined. EG significantly increased urine parameters (pH, volume, calcium, phosphorus, uric acid, and protein), blood urea, creatinine, and liver enzymes (P < 0.05). EG decreased creatinine clearance and urine magnesium and caused crystalluria. Treatment with BPE or cystone mitigates EG's effect; BPE was more potent than cystone (P < 0.05). BPE increases urine volume, sodium, and magnesium compared to the control and EG treated groups. BPE reduces proteinuria and prevents AKI, crystalluria, liver injury, and histopathological changes in the kidney tissue caused by EG. BPE might have a protective effect against EG-induced AKI, crystalluria, proteinuria, and stone deposition, most likely by its antioxidant content and activity.

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Effect of bee pollen extract on lead-induced toxicity in rat testis

Cited by 5 publications

References 53 publications

Impact of Maternal Exposure to Lead Acetate before Pregnancy Through Lactation Period on the Testicular histomorphologic Indices of Male Offspring of Wistar Rats, A Stereological Study

Impact of Maternal Exposure to Lead Acetate before Pregnancy Through Lactation Period on the Testicular histomorphologic Indices of Male Offspring of Wistar Rats, A Stereological Study

Protective Effect of Lemongrass Extract ( Cymbopogon nardus) on Spermatozoa Cells Induced by Lead Acetate

Protective effect of bee pollen in acute kidney injury, proteinuria, and crystalluria induced by ethylene glycol ingestion in rats

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