Effect of beta-mercaptoethanol or epidermal growth factor supplementation on in vitro maturation of canine oocytes collected from dogs with different stages of the estrus cycle

Kim, Min Kyu; Fibrianto, Yuda Heru; Oh, Hyun Ju; Jang, Goo; Kim, Hye Jin; Lee, Kyu Seung; Kang, Sung Keun; Lee, Byeong Chun; Hwang, Woo Suk

doi:10.4142/jvs.2004.5.3.253

Cited by 43 publications

(23 citation statements)

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“…Another issue, with varying opinions among researchers, is the influence of the stage of oestrus cycle in IVM of dog oocytes. In vitro meiosis of canine oocytes has been attributed by various authors to in vivo reproductive status of ovary donors (Yamada et al 1993;Luvoni et al 2001;Kim et al 2004). Other authors have reported that oocytes retrieved from ovaries of bitches at various reproductive phases of the oestrus cycle can progress into meiosis (Songsasen and Wildt 2005), and develop into early embryos up to and including the 8-cell stage (Rodrigues et al 2004).…”

Section: Discussionmentioning

confidence: 99%

Preliminary Study in Immature Canine Oocytes Stained with Brilliant Cresyl Blue and Obtained From Bitches with Low and High Progesterone Serum Profiles

Rodrigues

Rodriguez

Silva

et al. 2009

Reprod Domestic Animals

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This study was conducted: (i) to observe the features and levels of blue colour impregnation in morphologically selected immature canine cumulus oocyte complexes (COCs) stained with the brilliant cresyl blue (BCB) dye, as indicators of quality, and integrity of nuclear oocyte chromatin configuration before in vitro maturation (IVM); (ii) to observe the relationship between the influence of serum progesterone (SP) concentrations from ovary donors and BCB staining of immature dog oocytes. The results showed that out of 138 canine COCs, germinal vesicle (GV) stage prevailed in BCB+ oocytes at percentages of 67.4% (60/89), which were statistically higher than those observed in BCB+/- (52.2%; 23/44) and BCB- (20%; 1/5) oocytes (p = 0.023). Oocytes BCB+ were interpreted as those having completed their growth and therefore possessing the capacity to mature and develop in vitro. Ooplasm and cumulus cells (CCs) of canine oocytes were BCB staining independent. Ooplasm blue colour staining reaction varied between grown oocytes, revealing different levels of glucose-6-phosphate dehydrogenase activity among and within oocytes. Additionally, SP profile of ovary donors was not a relevant indicator for selection of oocytes screened with the BCB stain. Similar numbers of high quality oocytes were observed to be BCB+, BCB+/- and BCB- between groups of females with SP varying from 0 to 2.5 ng/ml (n = 5), and those with SP varying from 2.6 to 16.7 ng/ml (n = 4) (p = 0.680). It may be inferred that bitches with low and high SP profiles have grown oocytes in their ovaries, as determined by the BCB absorbance in their ooplasms.

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Section: Discussionmentioning

confidence: 99%

Preliminary Study in Immature Canine Oocytes Stained with Brilliant Cresyl Blue and Obtained From Bitches with Low and High Progesterone Serum Profiles

Rodrigues

Rodriguez

Silva

et al. 2009

Reprod Domestic Animals

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“…Nuclear chromatin was evaluated under epifluorescence microscopy (Nikon Eclipse 600 (NIKON Instruments, Florence, Italy) equipped with B‐2 A 460 nm excitation and 346 nm emission filters). Chromatin configurations were classified as previously described (Kim et al. 2004).…”

Section: Methodsmentioning

confidence: 99%

“…Nuclear chromatin was evaluated under epifluorescence microscopy (Nikon Eclipse 600 (NIKON Instruments, Florence, Italy) equipped with B-2 A 460 nm excitation and 346 nm emission filters). Chromatin configurations were classified as previously described (Kim et al 2004). Stages indicative of meiosis resumption were: germinal vesicle break down (GVBD) (organization and condensation of chromatin into chromosomes), metaphase I (alignment of chromosomes on meiotic spindle) and metaphase II, which was indicated by the presence of the 2nd metaphase plate (MII) and the first polar body (PB) extruded.…”

Section: Nuclear Chromatin Evaluationmentioning

confidence: 99%

Expression of the μ Opioid Receptor and Effects of the Opioid Antagonist Naloxone on In Vitro Maturation of Oocytes Recovered from Anoestrous Bitches

Iorga

Valentini

Santis

et al. 2009

Reprod Domestic Animals

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The mu-opioid receptor (MOR) is expressed in bovine, human, equine and canine oocytes, and in seasonal breeders, it is expressed with higher intensity during the anoestrous phase. Supplementation of in vitro maturation (IVM) medium with opioid agents, agonists or antagonists, was shown to affect oocyte maturation in several species such as rat, bovine and equine. This study reports the effects of supplementing IVM medium with naloxone (Nx), an opioid antagonist, on nuclear and cytoplasmic maturation rate of oocytes recovered from anoestrous bitches. Cytoplasmic maturation was examined in terms of mitochondrial (mt) distribution. In order to confirm the receptor-mediated action of Nx, in oocytes of anoestrous bitches, MOR expression was analyzed by Western blot. Cumulus-oocyte complexes, recovered from the ovaries of bitches in anoestrous, were cultured in vitro and Nx was added at the concentrations of 1 x 10(-6), 1 x 10(-8) and 1 x 10(-10) M. The rate of oocytes resuming meiosis after culture in presence of 1 x 10(-6) M Nx (29%) was significantly higher than that of oocytes of control group (12%; p < 0.05). However, treatment with Nx did not affect mt distribution pattern. In denuded oocytes and in corresponding cumulus cells, a doublet of 65 and 50 kDa was observed. We conclude that, in oocytes of anoestrous bitches, MOR is expressed and Nx significantly improves nuclear maturation rate. Further studies should be performed to elucidate the expression of other opioid receptors, such as delta and kappa, and possible interactive effects of their antagonists on canine oocyte maturation.

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“…fetal calf, cow or bitch, in oestrus or not; Robertson et al 1992;Hewitt et al 1998;Rodrigues and Rodrigues 2003;Oh et al 2005;Lee et al 2007c); numerous growth factors such as IGF-1, epidermal growth factor (EGF) and human or porcine somatotropin (Rodrigues and Rodrigues 2003;Kim et al 2004;Bolamba et al 2006;Cui et al 2006;Rodrigues et al 2006;Hatoya et al 2009); various protein sources (Songsasen et al 2002;Rodrigues and Rodrigues 2003); hyaluronan (Rodrigues et al 2006); antioxidants (Kim et al 2004;Rota and Cabianca 2004;Hossein et al 2007;Lee et al 2007b); and cell cycle inhibitors (Hanna et al 2008). Nevertheless, Alhaider and Watson (2009) recently obtained high metaphase rates (up to 49%) with a combination of fibroblast growth factor (FGF), IGF, growth factor (GF) and transforming growth factor (TGF)-a.…”

Section: In Vitro Maturation Of Oocytesmentioning

confidence: 99%

The canine oocyte: uncommon features of in vivo and in vitro maturation

Chastant-Maillard

Lesegno

Chebrout

et al. 2011

Reprod. Fertil. Dev.

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The biology of the canine oocyte is unusual compared with that of other mammalian females. The present paper reviews both in vivo and in vitro specificities of canine oocytes. Final follicular growth in the bitch is characterised by an early appearance of LH binding sites in the granulosa, a high proportion of polyovular follicles and a preovulatory luteinisation, starting at the time of the LH surge. Through follicular fluid, preovulatory oocytes are thus exposed to high levels of progesterone, as high as 1000-fold plasma concentrations. The composition of the follicular fluid is affected by the size of the female. The more specific aspect of oocyte biology in the bitch is ovulation: oocytes are expelled immature, at the Prophase I stage. Ovulatory follicles are 6-8 mm in diameter, releasing oocytes from 110 µm, with dark cytoplasm. Resumption of meiosis occurs from 48 h postovulation, MII stages appearing 48-54 h after ovulation. The mechanisms controlling such a late meiotic resumption are still unknown. Granulosa cells seem to play a central role as in other mammalian species, but not with cAMP as the principal mediator. The importance of a transient reactivation of oocyte transcription a few hours before meiotic resumption is to be explored. These specific features may contribute to the low efficiency of IVM. Only 10-20% oocytes reach the metaphase stage and suffer from a poor cytoplasmic maturation. Moreover, in vitro culture of canine oocytes is associated with a high proportion of degeneration. To date, IVM of the oocytes is the main limiting factor for the development of assisted reproductive techniques in the canine. A better knowledge of the basic physiology of folliculogenesis and the molecular mechanisms controlling oocyte meiosis resumption in this species may allow us to overcome this obstacle.

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Effect of beta-mercaptoethanol or epidermal growth factor supplementation on in vitro maturation of canine oocytes collected from dogs with different stages of the estrus cycle

Cited by 43 publications

References 0 publications

Preliminary Study in Immature Canine Oocytes Stained with Brilliant Cresyl Blue and Obtained From Bitches with Low and High Progesterone Serum Profiles

Preliminary Study in Immature Canine Oocytes Stained with Brilliant Cresyl Blue and Obtained From Bitches with Low and High Progesterone Serum Profiles

Expression of the μ Opioid Receptor and Effects of the Opioid Antagonist Naloxone on In Vitro Maturation of Oocytes Recovered from Anoestrous Bitches

The canine oocyte: uncommon features of in vivo and in vitro maturation

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