The current study aimed to investigate the Response of Bacillus megaterium and Bacillus mucilaginosus strains on growth attributes and uptake of soybean (Glycine max (L.) Merrill.) at All India Coordinated Research Project (AICRP) on Integrated Farming System Research at College of Agriculture, Indore, Madhya Pradesh, India during kharif, 2019 & 20. The study was carried out in randomized block design (RBD) with 8 treatments, viz. Control with 75% RDF (T1), Control with 100% RDF (T2), 75% RDF with Bacillus megaterium and Bacillus mucilaginosus strains as seed treatment (T3), 100% RDF with Bacillus megaterium and Bacillus mucilaginosus strains as seed treatment (T4), 75% RDF with Bacillus megaterium and Bacillus mucilaginosus strains as foliar application at 30 and 45 DAS (T5), 100% RDF with Bacillus megaterium and Bacillus mucilaginosus strains as foliar application at 30 and 45 DAS (T6), 75% RDF with Bacillus megaterium and Bacillus mucilaginosus strains as seed treatment + foliar application of Bacillus megaterium and Bacillus mucilaginosus strains at 30 and 45 DAS (T7) and 100% RDF with Bacillus megaterium and Bacillus mucilaginosus strains as seed treatment + foliar application of Bacillus megaterium and Bacillus mucilaginosus strains at 30 and 45 DAS (T8). Among all the treatments, growth parameters, NPK uptake by grain and straw and total uptake by crop were observed highest with 100% RDF with Bacillus megaterium and Bacillus mucilaginosus as seed treatment + foliar application of Bacillus megaterium and Bacillus mucilaginosus at 30 and 45 DAS.