Effect of bleaching agent extracts on murine macrophages

Fernandes, Antônio Carlos; Vilela, Polyana G F; Valera, Márcia Carneiro; Bolay, Carola; Hiller, Karl Anton; Schweikl, Helmut; Schmalz, Gottfried

doi:10.1007/s00784-017-2273-1

Cited by 5 publications

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“…In contact with human cells, -OH radical can induce lipid peroxidation and DNA alteration followed by cell lysis and death [ 5 ]. Previous in vitro study [ 10 ] showed irreversible cytotoxic effects in cells exposed to 20% CP for 1 h. However, although no genotoxicity effect of lower concentration of CP was used [ 6 , 11 – 13 ], a closer view of clinical studies that evaluated higher concentrated bleaching materials for at-home technique showed controversial results in terms of micronucleus frequency [ 11 , 13 – 15 ]. Genotoxicity is the ability of a substance to damage the human cells genetic content [ 16 ].…”

Section: Introductionmentioning

confidence: 99%

Influence of smoking on oral cells genotoxicity after at-home bleaching using 22% carbamide peroxide: a cohort study

et al. 2021

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Objective This study evaluated the influence of smoking on the oral cells genotoxicity before and after at-home bleaching using 22% carbamide peroxide (CP). Materials and methods This is a prospective observational analytics cohort study which evaluated nonsmokers (NS; n = 24) and smokers (S; n = 16) patients. At-home bleaching was performed using 22% CP gel in individual trays for 1 h per day for 14 days in both groups. Scrapped cells from marginal gums were collected before the bleaching treatment (D0- baseline ) and 1 day (D1), 15 days (D15), and 1 month (D30) after its finishing. Cells were stained with Giemsa 10%, and the micronucleus (MN) and metanuclear alterations (MA) were counted by a trained operator in 1000 cells per patient. The collections and data analysis occurred blindly. Data was analyzed by Kruskal–Wallis, Dunn, and Mann–Whitney test (α = 0.05). Results MN frequency was not influenced by smoking or bleaching. An increase of MA was observed between D0 and D30 for both groups ( p < 0.001); however, no statistical difference was found between NS and S ( p > 0.05) in the evaluation times. Conclusion Smoking associated with 22% carbamide peroxide gel for at-home bleaching does not show genotoxic potential analyzed by the MN counts. However, a significant increase of MA was found for smokers and nonsmokers. Clinical relevance Despite of the increase in MA, smoking associated with 22% CP peroxide at-home bleaching showed no important genotoxic potential (MN) for oral cells. Therefore, at-home bleaching treatment is safe for nonsmokers and smokers even with a high carbamide peroxide concentration of 22%.

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Section: Introductionmentioning

confidence: 99%

Influence of smoking on oral cells genotoxicity after at-home bleaching using 22% carbamide peroxide: a cohort study

et al. 2021

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Redox regulation of immunity and the role of small molecular weight thiols

Ghezzi

2021

Redox Biology

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Radiation Phenomena: Some Natural Sources, Mechanisms of Effects, Ways of Biological Organisms Protection and Rehabilitation

KLYUCHKO

2023

Biotechnol. acta

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Radiation is an important and dangerous factor in contemporary reality in some regions of industrial countries, after technological accidents at nuclear objects, chemical enterprises, etc. This is also the reality of some contemporary military activities and armed conflicts. Radiation damages of organisms can arise also due to the natural reasons — aviation or space flights at high altitudes or even long stay on mountain heights. Natural reasons of such effects have been studied insufficiently for today. Purpose. To outline briefly some results of studies of the characteristics of ionizing radiation at different heights above the Earth. To describe briefly the influence of radiation factors on biological organisms and main mechanisms of these effects. To describe effects that cause pathological changes in organisms of people exposed to the low doses of radiation for a long time and methods of post-radiation rehabilitation of affected people in highlands conditions. Methods. Space satellite exploring of the Earth atmosphere at different altitudes above sea level with measurements of different characteristics of solar and galactic radiation (mainly X-ray, gamma radiation, as well as other types of ionizing radiation in some other ranges). Comparative analysis of the results of longterm observation of patients in hospital conditions using many standard laboratory methods of their states examinations. The conducted scientific research consisted of a complex of methodological techniques and approaches: clinical and physiological studies of respiratory and cardiovascular systems, hematological and immunological states, and functional state of higher nervous activity, mental and neurotic state; administration of antihypoxants, histochemical, biophysical and other methods were used to evaluate oxybiotic processes. Mathematical processing of the results, as well as methods of mathematical modeling was applied. Results. The results of the measurements of ionizing radiation levels during the satellite exploring of the Earth atmosphere at different altitudes were analyzed and presented in schemes. The mechanisms of damaging radiation effects in organisms at nano level were described: water radiolysis, “oxygen effect” as radio sensitizer, formation of various types of free radicals and peroxides with future consequences for organic compounds, cells, tissues, organs, and organisms. The results of medical treatment and rehabilitation at the EMBS of the persons irradiated by the low doses of radiation were presented, observed and discussed. Many of represented results were obtained thanks to the collective work of the great commands of our predecessors in science who searched for the possibilities of medical treatment and rehabilitation of patients who obtained low doses of radiation during long time. The contemporary results of possibilities of some developed pathological states pharmacological corrections were discussed; practical recommendations were done. Conclusions. Some of results of fulfilled works, which can be valuable in the treatment and rehabilitation of people of various contingents exposed to low doses of radiation of various natures for a long time, were presented. The outlined recommendations can be offered to persons of various radiation risk contingents for the purposes of their rehabilitation, in practice of health care, etc.

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Effect of bleaching agent extracts on murine macrophages

Abstract: The addition of an antioxidant can downregulate unfavorable effects of dental bleaching.

Cited by 5 publications

References 52 publications

Influence of smoking on oral cells genotoxicity after at-home bleaching using 22% carbamide peroxide: a cohort study

Influence of smoking on oral cells genotoxicity after at-home bleaching using 22% carbamide peroxide: a cohort study

Redox regulation of immunity and the role of small molecular weight thiols

Radiation Phenomena: Some Natural Sources, Mechanisms of Effects, Ways of Biological Organisms Protection and Rehabilitation

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