Effect of Bleaching on Mercury Release from Amalgam Fillings and Antioxidant Enzyme Activities: A Pilot Study

Çakır, Filiz Yalçın; Ergin, Esra; Gürgan, Sevil; Sabuncuoğlu, Suna; Arpa, Çiğdem; Tokgöz, İlknur; Özgüneş, Hilâl; Kiremitçi, Arlin

doi:10.1111/jerd.12092

Cited by 9 publications

(3 citation statements)

References 35 publications

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“…There are many studies in the literature investigating the effects of Hg on antioxidant activity systems in various tissues and fluids such as saliva and plasma ( 26 , 27 ). While some of these studies found a significant relationship between Hg and antioxidants, some found no significant relationship ( 17 , 26 ). Because Hg can produce reactive oxygen species, it can affect antioxidant enzymes such as SOD-1 and catalase ( 28 ).…”

Section: Discussionmentioning

confidence: 96%

“…Hg is found in the blood like other body fluids, but its levels in the blood are quite low compared to others. Even in industrial workers exposed to high levels of Hg, measured Hg concentrations have been reported to be in the parts per billion (ppb) range ( 17 ). In the present study, serum Hg concentrations reached the highest level 21 days after treatment (0.91 ng/mL), but no statistically significant difference was found among time periods (p=0.072).…”

Section: Discussionmentioning

confidence: 99%

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Release of mercury from amalgam filling and its relationship with metallothionein and superoxide dismutase

Gül¹,

Karataş²,

Sağsöz³

et al. 2022

eor

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This study aims at determining the amount of mercury released over time from amalgam after treatment in healthy subjects and to examine the relation of mercury with serum MT-1 and SOD-1 levels. Materials and MethodsAmalgam filling was applied to the 15 subjects aged 19-22 years and blood samples were collected before treatment and 1 day, 7 days, 21 days and 35 days after treatment. Mercury analysis of serum samples was performed using Inductively Coupled Plasma Mass Spectrometry (ICP-MS). In addition, MT-1 and SOD-1 levels in serum samples were measured using commercial enzyme-linked immunosorbent assay (ELISA). Friedman test and Spearman's correlation analysis was performed to analyse the data. p value was interpreted in significance level of 0.05. ResultsAs a result of the analysis for MT-1, it was found that the values decreased over time and this decrease was statistically significant after 21 days (p<0.05). In addition, it was found that SOD-1 decreased over time, but this decrease was not statistically significant . In terms of released mercury, there was no statistically significant difference among the values of mercury released over time . According to the results of correlation analysis, no statistically significant relationship was found among the variables. ConclusionThe results of the present study indicated that the amount of mercury released from the tested amalgam were found to be tolerable and no significant relationship was found between MT-1 and SOD-1.

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Section: Discussionmentioning

confidence: 96%

Section: Discussionmentioning

confidence: 99%

Release of mercury from amalgam filling and its relationship with metallothionein and superoxide dismutase

Gül¹,

Karataş²,

Sağsöz³

et al. 2022

eor

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show abstract

“…The enzymatic reactions are important for the life of the organisms and enzymes assist life processes in all life forms from viruses to man. These reactions have been naturally designed to function under various physiological conditions [ 1 ], for replacement of old tissues [ 2 ], conversion of food into energy [ 3 ] and disposal of toxic materials [ 4 ].…”

Section: Introductionmentioning

confidence: 99%

Purification and characterization of L-arginine deiminase from Penicillium chrysogenum

El-Shora,

El-Zawawy,

El-Rheem

et al. 2024

BMC Microbiol

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L-arginine deiminase (ADI, EC 3.5.3.6) hydrolyzes arginine to ammonia and citrulline which is a natural supplement in health care. ADI was purified from Penicillium chrysogenum using 85% ammonium sulfate, DEAE-cellulose and Sephadex G200. ADI was purified 17.2-fold and 4.6% yield with a specific activity of 50 Umg− 1 protein. The molecular weight was 49 kDa. ADI expressed maximum activity at 40oC and an optimum pH of 6.0. ADI thermostability was investigated and the values of both t0.5 and D were determined. Kd increased by temperature and the Z value was 38oC. ATP, ADP and AMP activated ADI up to 0.6 mM. Cysteine and dithiothreitol activated ADI up to 60 µmol whereas the activation by thioglycolate and reduced glutathione (GSH) prolonged to 80 µmol. EDTA, α,α-dipyridyl, and o-phenanthroline inactivated ADI indicating that ADI is a metalloenzyme. N-ethylmaleimide (NEM), N-bromosuccinimide (NBS), butanedione (BD), dansyl chloride (DC), diethylpyrocarbonate (DEPC) and N-acetyl-imidazole (NAI) inhibited ADI activity indicating the necessity of sulfhydryl, tryptophanyl, arginyl, lysyl, histidyl and tyrosyl groups, respectively for ADI catalysis. The obtained results show that ADI from P. chrysogenum could be a potential candidate for industrial and biotechnological applications.

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Biocompatibility and functionality of dental restorative materials

Zabrovsky

Beyth

Pietrokovski

et al. 2017

Biocompatibility of Dental Biomaterials

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Effect of Bleaching on Mercury Release from Amalgam Fillings and Antioxidant Enzyme Activities: A Pilot Study

Abstract: Bleaching treatments with the systems tested in this pilot study have no deleterious effect on the mercury release from amalgam fillings and antioxidant enzymes in body fluids.

Cited by 9 publications

References 35 publications

Release of mercury from amalgam filling and its relationship with metallothionein and superoxide dismutase

Release of mercury from amalgam filling and its relationship with metallothionein and superoxide dismutase

Purification and characterization of L-arginine deiminase from Penicillium chrysogenum

Biocompatibility and functionality of dental restorative materials

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