Effect of c-Sterilization Process on PLGA Microspheres Loaded with                Insulin-Like Growth Factor - I (IGF-I)

Carrascosa, Carles Navarro; Espejo, L. A.; Torrado, Susana; Torrado, J. J.

doi:10.1177/088532803038026

Cited by 40 publications

(25 citation statements)

References 33 publications

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“…Calculation of the similarity factor (f 2 ) between both release profiles gave a value lower than 50 (37.47), thereby indicating that the release profiles cannot be considered similar. Other authors have also shown that γ-irradiation affected the drug release rate 31 . For instance PLGA decomposition and the decrease in the average molecular weight increased both the extent and the rate of bupivacaine released from PLGA microspheres 32 .…”

Section: Resultsmentioning

confidence: 94%

Effect of Gamma-Irradiation on Biodegradable Microspheres Loaded With Rasagiline Mesylate

Fernández

Barcia

Negro

2016

J. Chil. Chem. Soc.

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In the present study, the influence of gamma-irradiation was evaluated on the physicochemical characteristics and in vitro release of rasagiline mesylate (RM), a selective MAO-B inhibitor used in Parkinson's disease, from poly(D,L-lactide-co-glycolide) (PLGA) microspheres. Microspheres were prepared using PLGA 50:50 by the solvent evaporation technique (O/W emulsion). Microspheres were sterilized by gamma-irradiation and their influence was assessed by scanning electron microscopy (SEM), laser light diffraction, differential scanning calorimetry (DSC), X-ray diffraction (XRD), gel permeation chromatography (GPC), encapsulation efficiency (EE) and in vitro drug release. Gamma-irradiation of RM-loaded microspheres did not affect EE, DSC and XRD patterns. After gamma-irradiation, changes on the surface were observed by SEM, but no significant difference in mean particle size was observed. GPC measurements showed a decrease in molecular weight of the polymer after five days of in vitro release. The similarity factor value between irradiated and non-irradiates microspheres was <50, indicating the non-similarity of the release profiles. The sterilization technique had an effect on the integrity of polymeric system, significantly affecting in vitro release of RM from PLGA microspheres. Therefore, from our results we conclude that gamma-irradiation is not a suitable sterilization procedure for this formulation.

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Section: Resultsmentioning

confidence: 94%

Effect of Gamma-Irradiation on Biodegradable Microspheres Loaded With Rasagiline Mesylate

Fernández

Barcia

Negro

2016

J. Chil. Chem. Soc.

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“…Other studies reported that the osteoinductive potential of bone morphogenetic proteins of various origins was preserved after sterilization at 25 kGy (27,28). Growth factors react differently to gamma irradiation: gamma sterilization alters the properties of insulin-like growth factor-1 (29), whereas the TGF-β superfamily is resistant to high doses of radiation. Our data suggest that gamma irradiation does not activate, but rather maintains, the intrinsic activity of BCM to induce changes in gene expression.…”

Section: Discussionmentioning

confidence: 99%

High-dose irradiation of bone chips preserves the <i>in vitro</i> activity of bone-conditioned medium

et al. 2016

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Extracorporeal irradiation sterilizesresected tumor bone used as autografts in reconstruction surgery. Therapeutic irradiation is a standard technique in head and neck cancer therapy that aims to preserve organ function. Bone irradiation has a complex, mostly inhibitory, effect on remodeling and regeneration, although the underlying mechanisms are still not fully understood. It remains unclear if extracorporeal irradiation affects the paracrine-like activity of the corresponding autografts. We recently reported that bone-conditioned medium from autogenous bone chips contains a number of factors that might affect cell activity. In the present study, we investigated the effects of extracorporeal irradiation of porcine cortical bone chips on the activity of the corresponding bone-conditioned medium. The effects of bone-conditioned medium on the expressions of transforming growth factor-beta (TGF-β) target genes in oral fibroblasts were assessed. Bone-conditioned medium from bone chips exposed to a total radiation dose up to 120 Gy did not affect expressions of TGF-β target genes, including adrenomedullin, BTB/ POZ domain-containing protein 11, proteoglycan 4, NADPH oxidase 4, and interleukin 11, in oral fibroblasts. In conclusion, bone irradiation does not alter the capability of the corresponding bone-conditioned medium to provoke a robust fibroblastic cell response in vitro. (J Oral Sci 58, 325-331, 2016)

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“…The process can be undertaken with a wide range of polymers, and is relatively easy to scale-up (Iqbal et al, 2015). The oil-in-water emulsion and double emulsion (w 1 /o/w 2 ) methods are most widely explored, although sterilization can be challenging and low encapsulation efficacy can be an issue as proteins are able to diffuse from the oil phase to the continuous aqueous phase (Carrascosa et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

Liraglutide-loaded poly(lactic-co-glycolic acid) microspheres: Preparation and in vivo evaluation

Williams

Branford-White

et al. 2016

European Journal of Pharmaceutical Sciences

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In this work, we sought to generate sustained-release injectable microspheres loaded with the GLP-1 analogue liraglutide. Using water-in-oil-in-water double emulsion methods, poly(lactic-co-glycolic acid) (PLGA) microspheres loaded with liraglutide were prepared. The microspheres gave sustained drug release over 30days, with cumulative release of up to 90% reached in vitro. The microspheres were further studied in a rat model of diabetes, and their performance compared with a group given daily liraglutide injections. Reduced blood sugar levels were seen in the microsphere treatment groups, with the results being similar to those obtained with conventional injections between 10 and 25days after the commencement of treatment. After 5 and 30days of treatment, the microspheres seem a little slower to act than the injections. The pathology of the rats' spleen, heart, kidney and lungs was probed after the 30-day treatment period, and the results indicated that the microspheres were safe and had beneficial effects on the liver, reducing the occurrence of fatty deposits seen in untreated diabetic rats. Moreover, in terms of liver, renal and cardiac functions, and blood lipid and antioxidant levels, the microspheres were as effective as the injections. The expression of several proteases linked to the metabolism of aliphatic acids and homocysteine was promoted by the microsphere formulations. Inflammatory markers in the microsphere treatment groups were somewhat higher than the injection group, however. The liraglutide/PLGA microspheres prepared in this work are overall shown to be efficacious in a rat model of diabetes, and we thus believe they have strong potential for clinical use.

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Effect of c-Sterilization Process on PLGA Microspheres Loaded with Insulin-Like Growth Factor - I (IGF-I)

Cited by 40 publications

References 33 publications

Effect of Gamma-Irradiation on Biodegradable Microspheres Loaded With Rasagiline Mesylate

Effect of Gamma-Irradiation on Biodegradable Microspheres Loaded With Rasagiline Mesylate

High-dose irradiation of bone chips preserves the <i>in vitro</i> activity of bone-conditioned medium

Liraglutide-loaded poly(lactic-co-glycolic acid) microspheres: Preparation and in vivo evaluation

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