Effect of caffeine on oxidation susceptibility of human plasma low density lipoproteins

Kriško, Anita; Kveder, Marina; Pifat, Greta

doi:10.1016/j.cccn.2004.12.001

Cited by 31 publications

(24 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The solution was irradiated by UV light (254 nm, 0.65 mW/cm 2 ) for 12 hours at 4°C (UVGL-15 compact UV lamp, UVP). Oxidation was confirmed by a 3-fold increase of absorbance at 234nm, as previously observed [21].…”

Section: Oxidation Of Low Density Lipoproteinsupporting

confidence: 83%

See 1 more Smart Citation

Modulation of endothelial cell thrombomodulin by PPAR ligands — Variation according to environment

Mangan

Clancy

Golledge

2008

Thrombosis Research

View full text Add to dashboard Cite

show abstract

Section: Oxidation Of Low Density Lipoproteinsupporting

confidence: 83%

“…Low density lipoprotein was oxidized as previously described [21]. Briefly LDL (Calbiochem, Merk Pty Ltd) was resuspended to a final concentration of 2mg/mL in PBS and 2µM CuSO 4 .…”

Section: Oxidation Of Low Density Lipoproteinmentioning

confidence: 99%

Modulation of endothelial cell thrombomodulin by PPAR ligands — Variation according to environment

Mangan

Clancy

Golledge

2008

Thrombosis Research

View full text Add to dashboard Cite

show abstract

“…6,7 ROS regulate cell proliferation, apoptosis, and migration, as well as inflammatory gene expression, lowdensity lipoprotein oxidation, 8 and inactivation of vasoactive compounds, such as endothelial nitric oxide. 9 Although RS generation is a fundamental component of metabolism and signal transduction, 10 unchecked RS can provoke a variety of damage within the cell.…”

mentioning

confidence: 99%

The Methyl Xanthine Caffeine Inhibits DNA Damage Signaling and Reactive Species and Reduces Atherosclerosis in ApoE ^−/− Mice

Mercer

Gray

Figg

et al. 2012

ATVB

View full text Add to dashboard Cite

Objective-Caffeine remains one of the most widely consumed drugs in the world. Caffeine has multiple actions, including inhibition of the DNA damage response, and its metabolites, 1-methylxanthine and 1-methyluric acid, are potent antioxidants. Combined, these properties can exert direct effects on cell proliferation, cell death, inflammation, and DNA repair, all important processes that occur in atherosclerosis. Methods and Results-We first examined the effects of caffeine on mouse vascular smooth muscle cells. Caffeine inhibited activation of the DNA damage response regulator ataxia telangiectasia mutated protein and its downstream targets. Caffeine delayed DNA repair, had a concentration-dependent effect on cell proliferation, and protected against apoptosis. In vitro caffeine reduced oxygen consumption and decreased generation of reactive oxygen species. In vivo caffeine reduced DDR activation in vascular and nonvascular tissues, reduced reactive nitrogen species and serum levels of the DNA adduct 8-oxo-guanine, and inhibited atherogenesis in fat−fed ApoE −/− mice. Reduction in atherosclerosis was independent of the effects on blood pressure and serum lipids but associated with reduced cell proliferation and ataxia telangiectasia mutated protein activation. Conclusion-The Methyl Xanthine caffeine inhibits the DNA damage response in vitro and in vivo, regulates both cell proliferation and apoptosis after DNA damage, inhibits reactive species, and reduces atherogenesis in ApoE

show abstract

“…There are also reports suggesting that caffeine is capable of inducing certain forms of oxidative damage by increasing lipid peroxidation (Dianzani et al, 1991). On the other hand, caffeine has been reported as a protective substance in cellular damage (Kamat et al, 2000;Krisko et al, 2005) with beneficial antioxidant effects (Nikolic et al, 2003) probably due to the main metabolites of caffeine, 1-methylxanthine and 1-methyluric acid, which are highly effective antioxidants (Lee, 2000). So, the real behavior of caffeine is unclear.…”

Section: Resultsmentioning

confidence: 97%

Gender differences in biochemical markers and oxidative stress of rats after 28 days oral exposure to a mixture used for weight loss containing p-synephrine, ephedrine, salicin, and caffeine

Schmitt

Arbo

Lorensi

et al. 2016

Braz. J. Pharm. Sci.

View full text Add to dashboard Cite

The association of p-synephrine, ephedrine, salicin, and caffeine in dietary supplements and weight loss products is very common worldwide, even though ephedrine has been prohibited in many countries. The aim of this study was to evaluate a 28-day oral exposure toxicity profile of p-synephrine, ephedrine, salicin, and caffeine mixture (10:4:6:80 w/w respectively) in male and female Wistar rats. Body weight and signs of toxicity, morbidity, and mortality were observed daily. After 28 days, animals were euthanized and blood collected for hematological, biochemical, and oxidative stress evaluation. No clinical signs of toxicity, significant weight loss or deaths occurred, nor were there any significant alterations in hematological parameters. Biochemical and oxidative stress biomarkers showed lipid peroxidation, and hepatic and renal damage (p < 0.05; ANOVA/Bonferroni) in male rats (100 and 150 mg/kg) and a reduction (p < 0.05; ANOVA/Bonferroni) in glutathione (GSH) levels in all male groups. Female groups displayed no indications of oxidative stress or biochemical alterations. The different toxicity profile displayed by male and female rats suggests a hormonal influence on mixture effects. Results demonstrated that the tested mixture can alter oxidative status and promote renal and hepatic damages.Uniterms: p-Synephrine/toxicity profile. Ephedrine/toxicity profile. Salicin/toxicity profile. Caffeine/ toxicity profile. Dietary supplements/evaluation. Weight loss products/evaluation.A associação de p-sinefrina, efedrina, salicina, e cafeína em suplementos alimentares e produtos para perda de peso é muito utilizada em todo o mundo, embora a efedrina tenha sido proibida em muitos países. O objetivo deste estudo foi avaliar o perfil de toxicidade à exposição oral de 28 dias à associação de p-sinefrina, efedrina, salicina e cafeína (na proporção de 10:4:6:80 m/m respectivamente) em ratos Wistar machos e fêmeas. Diariamente, os animais foram observados quanto ao peso corporal, sinais de toxicidade, morbidade e mortalidade. Após 28 dias, os animais foram sacrificados e o sangue coletado para avaliações hematológicas, bioquímicas e de estresse oxidativo. Não se observaram sinais clínicos de toxicidade, tampouco perda significativa de peso, mortes, ou quaisquer alterações significativas nos parâmetros hematológicos. Biomarcadores do estresse oxidativo e bioquímicos mostraram peroxidação lipídica, danos renais e hepáticos (p < 0,05; ANOVA/Bonferroni) em ratos machos (100 e 150 mg/kg) e a redução (p < 0,05; ANOVA/Bonferroni) nos níveis de glutationa reduzida (GSH) em todos os grupos de machos tratados. Nas fêmeas, não houve indícios de estresse oxidativo, nem alterações bioquímicas. O diferente perfil de toxicidade entre os gêneros sugere influência hormonal nos efeitos de mistura administrada. A associação testada pode alterar o estado oxidativo e promover danos renais e hepáticos. Unitermos INTRODUCTIONOverweight and obesity are highly prevalent worldwide. Many consumers are seeking strategies for reducing weight to healt...

show abstract

Effect of caffeine on oxidation susceptibility of human plasma low density lipoproteins

Cited by 31 publications

References 19 publications

Modulation of endothelial cell thrombomodulin by PPAR ligands — Variation according to environment

Modulation of endothelial cell thrombomodulin by PPAR ligands — Variation according to environment

The Methyl Xanthine Caffeine Inhibits DNA Damage Signaling and Reactive Species and Reduces Atherosclerosis in ApoE ^−/− Mice

Gender differences in biochemical markers and oxidative stress of rats after 28 days oral exposure to a mixture used for weight loss containing p-synephrine, ephedrine, salicin, and caffeine

Contact Info

Product

Resources

About

Effect of caffeine on oxidation susceptibility of human plasma low density lipoproteins

Cited by 31 publications

References 19 publications

Modulation of endothelial cell thrombomodulin by PPAR ligands — Variation according to environment

Modulation of endothelial cell thrombomodulin by PPAR ligands — Variation according to environment

The Methyl Xanthine Caffeine Inhibits DNA Damage Signaling and Reactive Species and Reduces Atherosclerosis in ApoE −/− Mice

Gender differences in biochemical markers and oxidative stress of rats after 28 days oral exposure to a mixture used for weight loss containing p-synephrine, ephedrine, salicin, and caffeine

Contact Info

Product

Resources

About

The Methyl Xanthine Caffeine Inhibits DNA Damage Signaling and Reactive Species and Reduces Atherosclerosis in ApoE ^−/− Mice