Effect of capping agents on the cytotoxicity of silver nanoparticles in human normal and cancer skin cell lines

Netchareonsirisuk, Ponsawan; Puthong, Songchan; Dubas, Stephan Thierry; Palaga, Tanapat; Komolpis, Kittinan

doi:10.1007/s11051-016-3624-6

Cited by 19 publications

(15 citation statements)

References 36 publications

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“…Alginate and Poly 4-styrene-sulfonic acid-co-maleic acid (PSSMA) capping agents are selectively toxic to the cancer cell line but not to the normal cell line. These results confirm that one of the determining factors for toxicity of AgNPs is the type of capping agent [ 191 ]. Nevertheless, the question still remains whether AgNPs can affect keratinocytes and fibroblasts during the healing process.…”

Section: Strategies To Overcome Toxicitysupporting

confidence: 78%

Therapeutic strategies and potential implications of silver nanoparticles in the management of skin cancer

Dasari

Yedjou

Cruse

et al. 2020

Nanotechnology Reviews

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Skin cancer (SC) is the most common carcinoma affecting 3 million people annually in the United States and millions of people worldwide. It is classified as melanoma SC (MSC) and non-melanoma SC (NMSC). NMSC represents approximately 80% of SC and includes squamous cell carcinoma and basal cell carcinoma. MSC, however, has a higher mortality rate than SC because of its ability to metastasize. SC is a major health problem in the United States with significant morbidity and mortality in the Caucasian population. Treatment options for SC include cryotherapy, excisional surgery, Mohs surgery, curettage and electrodessication, radiation therapy, photodynamic therapy, immunotherapy, and chemotherapy. Treatment is chosen based on the type of SC and the potential for side effects. Novel targeted therapies are being used with increased frequency for large tumors and for metastatic disease. A scoping literature search on PubMed, Google Scholar, and Cancer Registry websites revealed that traditional chemotherapeutic drugs have little effect against SC after the cancer has metastasized. Following an overview of SC biology, epidemiology, and treatment options, this review focuses on the mechanisms of advanced technologies that use silver nanoparticles in SC treatment regimens.

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Section: Strategies To Overcome Toxicitysupporting

confidence: 78%

Therapeutic strategies and potential implications of silver nanoparticles in the management of skin cancer

Dasari

Yedjou

Cruse

et al. 2020

Nanotechnology Reviews

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“…65 Moreover, cytotoxicity against both normal and cancer cell lines has been reported to depend on the capping agent. 66 With reference to previous studies and experimental data obtained, it can be concluded that the cytotoxicity of silver nanoparticles depends on the capping agent. However, an interesting point that emerged from this investigation is the variation in cytotoxicity due to the biogenicity of silver nanoparticles.…”

Section: In Vitro Cytotoxicity Of Agnps Against Hct116 Colon Cancer Cmentioning

confidence: 83%

Mechanistic insight into the rapid one-step facile biofabrication of antibacterial silver nanoparticles from bacterial release and their biogenicity and concentration-dependent in vitro cytotoxicity to colon cells

et al. 2017

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“…Apoptosis induced by silver nanoparticles of different tumor cells has already been reported in vitro and ex vivo, which possibly occurred because of the translocation of the silver within the nucleus [ 83 , 84 , 85 , 86 ]. Franco-Molina et al reported that MCF-7 breast cancer cells treated with silver colloids exhibited considerably decreased dehydrogenase activity, causing NADH/NAD + reduction [ 87 ].…”

Section: Resultsmentioning

confidence: 99%

Synthesis of Functional Silver Nanoparticles and Microparticles with Modifiers and Evaluation of Their Antimicrobial, Anticancer, and Antioxidant Activity

Dilshad

Bibi

Sheikh

et al. 2020

JFB

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An accumulating body of evidence reports the synthesis and biomedical applications of silver nanoparticles. However, the studies regarding the use of maleic acid and citric acid in the synthesis of nano-sized silver particles (AgNPs) and micro-sized silver particles (AgMPs) as well as their antibacterial, antifungal, and anticancer activities have not been reported. In the current study, we synthesized AgNPs and AgMPs using maleic acid and citric acid as capping agents and have characterized them by UV-Vis, energy-dispersive X-Ray spectroscopy (EDS), X-Ray diffraction (XRD), and scanning electron microscope (SEM) analysis. The capped silver particles were examined for their antimicrobial activity and cytotoxicity against bacteria, fungi, and brine shrimp. Additionally, the anticancer activity of these particles was tested against human breast and liver cancer cell lines. The free radical scavenging activity of capped silver particles was evaluated by 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay. SEM analysis revealed a round plate-like morphology of maleic acid capped particles with an average size of 39 ± 4 nm, whereas citric acid capped particles display flower-shaped morphology with rough surfaces and an average size of 250 ± 5 nm. The uncapped AgMPs were hexagonal with 500 ± 4 nm size. EDS and XRD analysis confirmed the presence of Ag and face-centered cubic crystalline nature, respectively. Functionally, capped silver particles exhibited antibacterial activity against Gram-positive (Staphylococcus aureus, Bacillus subtilis, and Micrococcus luteus) and Gram-negative bacteria (Salmonella setubal, Enterobacter aerogenes, and Agrobacterium tumefaciens). The bactericidal activity was more active against Gram-negative bacteria with minimum inhibitory concentration (MIC) as low as 5 ppm as compared to 25 ppm for Gram-positive. Similarly, the silver particles demonstrated antifungal activity by inhibiting the growth of five fungal strains (Mucor species, Aspergillus niger, Aspergillus flavus, Aspergillus fumigatus, and Fusarium solani) up to 50% at the concentration of 500 ppm. Additionally, these particles showed substantial toxicity against brine shrimp and also significantly inhibited the proliferation of breast cancer (MCF7) and liver cancer (HePG2) cell lines (IC50 8.9–18.56 µM). Uncapped AgMPs were less effective, inhibiting only the proliferation of MCF7 cells with IC50 46.54 µM. Besides cytotoxicity, these particles acted as potential antioxidants, showing free radical scavenging up to 74.4% in a concentration-dependent manner. Taken together, our results showed that the modifiers affect the shape and size of silver particles and may, in part, contribute to the antimicrobial and antioxidant activity of silver particles. However, the contribution of maleic acid and citric acid in enhancing the antimicrobial, anticancer, and antioxidant potential independent of silver nano and microparticles needs to be studied further. In vivo experiments may determine the therapeutic effectiveness of silver particles capped with these modifiers.

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Effect of capping agents on the cytotoxicity of silver nanoparticles in human normal and cancer skin cell lines

Cited by 19 publications

References 36 publications

Therapeutic strategies and potential implications of silver nanoparticles in the management of skin cancer

Therapeutic strategies and potential implications of silver nanoparticles in the management of skin cancer

Mechanistic insight into the rapid one-step facile biofabrication of antibacterial silver nanoparticles from bacterial release and their biogenicity and concentration-dependent in vitro cytotoxicity to colon cells

Synthesis of Functional Silver Nanoparticles and Microparticles with Modifiers and Evaluation of Their Antimicrobial, Anticancer, and Antioxidant Activity

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