Effect of carbohydrate source on alfalfa somatic embryogenesis

Strickland, Steven G.; Nichol, James W.; McCall, Carol M.; Stuart, David A.

doi:10.1016/0168-9452(87)90138-5

Cited by 136 publications

(63 citation statements)

References 13 publications

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“…Morever, the maltose acts in a reversible way, suggesting that this carbohydrate serves as a nutritional effector of differentiation and morphogenesis. Our results eonfirm the striking effect of maltose obscrvcd in somatic cmbryogenesis (Strickland et al, 1987), androgcnesis (Kuhlmann and ForoughiWehr, 1989) and cmbryo conversion of alfalfa artificial seeds (Redenbaugh et al, 1987). However, the physiological rolc of maltose is stiJl poorly understood.…”

Section: Discussionsupporting

confidence: 61%

“…For improving the conversion of alfalfa embryos encapsulated in alginate beads into plantlcts, 3 % maltose and 1.5 mM ammonium were used as a substitute for sucrose. The conversion frcqueney was significantly increased (Strickland et al, 1987;Redenbaugh et al, 1987) and combination of maltose and starch were used in somalie embryogenesis for improving embryo induction (H un ter, 1987 ;Kuhlman and Foroughi-Wher, 1989).…”

Section: Introductionmentioning

confidence: 99%

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Effects of some organic compounds (maltose, sucrose, vitamins) on the shoot biomass ofAllocasuarina verticillata(Lam.) L. Johnson (Casuarinaceae) grownin vitro

Cao

Phelep

Duhoux

1990

Bulletin de la Société Botanique de France. Actualités Botaniqu

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Effects of sorne organic compounds (maltose, sucrose, vitamins) on the shoot biomass of Allocasuarina verticülata (Lam.) L. Johnson (Casuarinaceae) Summary.-Allocasuarina verticillata, a N 2 -fixing tree (actinorhizal plant) reknowned for ils tolerance to lire and salinity, is used for fuel wood production, land reclamation, shelter belis and for ornamental purposes. Root explants from seedlings were used for micropropagation. Biomass of regenerated shoots was increased by adding maltose (3 ta 6 %) instead of sucrose to Murashige and Skoog (1962) basal medium. This treatment reduced callogenesis and increased shoot number and chlorophyll content. The very high rate of shoot multiplication makes this species of Casuarinaceae a promising candidate for reforestation of arid soils in subtropical and mediterranean countries.Résumé.-Allocasuarina verticillata, plante actinorhizienne et arbre fixateur d'azote, connu pour sa tolérance au feu et à la salinité, est utilisé en pays tropical pour la production de charbon, la régénération des sols, et l'installation de brise-vents et de plantations ornementales. Des ex plants racinaires de jeunes plantes peuvent constituer un matériel apte à la micropropagation. La biomasse des tiges régénérées à partir des racines sur le milieu de base Mu rash ige et Skoog (1962) est considérablement augmentée si on remplace le saccharose par le maltose (3 à 6 %). Ce traitement réduit la callogenèse, augmente le nombre de tiges régénérées ainsi que leur contenu en chlorophylle. Le très fort coefficient de multiplication obtenu in vitro suggère d'utiliser cette espèce dans des essais de reboisement en zone tropicale et méditerranéenne.

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Section: Discussionsupporting

confidence: 61%

Section: Introductionmentioning

confidence: 99%

Effects of some organic compounds (maltose, sucrose, vitamins) on the shoot biomass ofAllocasuarina verticillata(Lam.) L. Johnson (Casuarinaceae) grownin vitro

Cao

Phelep

Duhoux

1990

Bulletin de la Société Botanique de France. Actualités Botaniqu

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“…For example, somatic embryo formation from nucellar calli of Citrus species could be stimulated when the sucrose in the induction medium was replaced by several carbohydrates such as galactose and lactose [37]. The superior effect of maltose over sucrose as a carbohydrate has also been reported for somatic embryo formation in alfalfa [38], indica rice [39]and creeping bentgrass [40]. In asparagus, the importance of carbohydrate type and concentration in the embryo induction medium has tured under the improved condition for 10 days showed normal growth without wilting, and more than 95% survived after they were cultured for 30 days.…”

Section: Carbohydrate Requirementsmentioning

confidence: 93%

Somatic Embryogenesis and Its Application for Breeding and Micropropagation in Asparagus (Asparagus officinalis L.).

Kunitake¹,

Mii

1998

Plant Biotechnology

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“…On the other hand, somatic embryogenesis was effective with glucose in Phaseolus coccineus (Genga and Allavena 1991); maltose in Alfalfa sp. (Strickland et al 1987;Denchev et al 1991) and Glycine max (Finer and McMullen 1991); and the culture maintenance in darkness for many crop species (Thorpe 1988). In contrast, somatic embryogenesis was predominantly seen when incubated at 16 L: 8D.…”

Section: Somatic Embryogenesismentioning

confidence: 99%

In vitro regeneration through organogenesis and somatic embryogenesis in pigeon pea [Cajanus cajan (L.) Millsp.] cv. JKR105

Krishna

Reddy

Ramteke

et al. 2011

Physiol Mol Biol Plants

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In vitro regeneration of pigeon pea through organogenesis and somatic embryogenesis was demonstrated with pigeon pea cv. JKR105. Embryonic axes explants of pigeon pea showed greater regeneration of shoot buds on 2.5 mg L −1 6-benzylaminopurine (BAP) in the medium, followed by further elongation at lower concentrations. Rooting of shoots was observed on half-strength Murashige and Skoog (MS) medium with 2 % sucrose and 0.5 mg L −1 3-indolebutyric acid (IBA). On the other hand, the regeneration of globular embryos from cotyledon explant was faster and greater with thidiazuron (TDZ) than BAP with sucrose as carbohydrate source. These globular embryos were maturated on MS medium with abscisic acid (ABA) and finally germinated on half-strength MS medium at lower concentrations of BAP. Comparison of regeneration pathways in pigeon pea cv. JKR105 showed that the turnover of successful establishment of plants achieved through organogenesis was more compared to somatic embryogenesis, despite the production of more embryos than shoot buds.

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Effect of carbohydrate source on alfalfa somatic embryogenesis

Cited by 136 publications

References 13 publications

Effects of some organic compounds (maltose, sucrose, vitamins) on the shoot biomass ofAllocasuarina verticillata(Lam.) L. Johnson (Casuarinaceae) grownin vitro

Effects of some organic compounds (maltose, sucrose, vitamins) on the shoot biomass ofAllocasuarina verticillata(Lam.) L. Johnson (Casuarinaceae) grownin vitro

Somatic Embryogenesis and Its Application for Breeding and Micropropagation in Asparagus (Asparagus officinalis L.).

In vitro regeneration through organogenesis and somatic embryogenesis in pigeon pea [Cajanus cajan (L.) Millsp.] cv. JKR105

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