Effect of Casein Phosphopeptide Amorphous Calcium Phosphate Conditioning on Microtensile Bond Strength of Three Adhesive Systems to Deep Dentin

Abstract

Objectives: This study aimed to assess the effect of casein phosphopeptide amorphous calcium phosphate (CPP-ACP) on microtensile bond strength of three adhesive systems to deep dentin.
 Materials and Methods: In this in vitro study, the occlusal surface of 30 sound human third molars was sectioned at 2 mm below and above the cementoenamel junction. The samples were randomly divided into three groups of OptiBond Solo Plus, G-Bond, and Clearfil SE Bond. Before composite resin bonding, each group was divided into two subgroups with and without dentin preparation with CPP-ACP. The teeth were then sectioned into 1 mm rods (n=15), and their microtensile bond strength was measured at a crosshead speed of 0.5 mm/min. Data were analyzed using two-way ANOVA and post-hoc Bonferroni test.
 Results: There was no significant difference in bond strength of OptiBond Solo Plus (P=0.44) and Clearfil SE Bond (P=0.67) with/without CPP-ACP. A significant difference was found between the two subgroups of G-Bond in this respect (P<0.001). The differences in microtensile bond strength among all subgroups were significant (P<0.05).
 Conclusion: The microtensile bond strength in use of G-Bond is significantly higher following dentin treatment with CPP-ACP compared with no use of CPP-ACP.

Evaluation of Casein Phosphopeptide–Amorphous Calcium Phosphate Effect on the Bond Strength of Self-etch Adhesives to Dentin: A Systematic Review

“…Out of the 13 studies included, two studies (15.4%) presented with a low risk of bias; nine studies (69.2%) presented a medium risk of bias, whereas the remaining two studies (15.4%) presented with a high risk of bias (Table 3). [12][13][14][15][16][17][18][19][20][21][22][23][24] None of the studies mentioned sample size estimation. Only two studies had mentioned about single operator carrying out all the experiments.…”

“…Four studies measured shear bond strength (SBS), eight studies measured tensile bond strength, and only one research discussed the interfaces between resin and dentin have been studied using field-emission scanning electron microscopes (FE-SEMs). [13][14][15][16][17][18][19][20][21][22][23][24] According to the results of the study by Barbosa-Martins et al, 16 pretreating demineralized dentin with remineralizing materials has been shown to be an effective, workable way to improve bonding while according to Doozandeh and Mirmohammadi 12 etching dentin affected by caries for up to 45 seconds could improve its SBS and bring it almost to that of normal dentin (ND). Pretreatment with MI Paste™ did not significantly affect the SBS of the etch-and-rinse adhesive in CAD.…”

“…(Contd...) considerably decreased following previous etching. According to Torkani et al, 23 the microtensile bond strength when using G-Bond™ (GB) is noticeably stronger after dentin treatment with CPP-ACP as opposed to when CPP-ACP is not used. As this is the first comprehensive analysis of how CPP-ACP affects the strength of self-etch adhesives' bond with dentin.…”