Effect of cathode properties on the thermophilic electrosynthesis of PolyHydroxyAlkanoates by Kyrpidia spormannii

Pillot, Guillaume; Sunny, Soniya; Comes, Victoria; Heussner, Alenica; Kerzenmacher, Sven

doi:10.1016/j.biteb.2022.101040

Cited by 4 publications

(5 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This modification had only little effect on the cell number of cathodic K. spormannii biofilms grown for three days. Although these results might be indicative of a hydrogen-independent electron uptake, it remains unclear whether the likewise improved oxygen reduction capability of the modified cathodes led to an elevated production of reactive oxygen species that hindered cathodic biofilm growth (Pillot et al 2022 ).…”

Section: Extremophilic Organisms Thriving On Cathodesmentioning

confidence: 99%

Electron transfer of extremophiles in bioelectrochemical systems

et al. 2022

View full text Add to dashboard Cite

The interaction of bacteria and archaea with electrodes is a relatively new research field which spans from fundamental to applied research and influences interdisciplinary research in the fields of microbiology, biochemistry, biotechnology as well as process engineering. Although a substantial understanding of electron transfer processes between microbes and anodes and between microbes and cathodes has been achieved in mesophilic organisms, the mechanisms used by microbes under extremophilic conditions are still in the early stages of discovery. Here, we review our current knowledge on the biochemical solutions that evolved for the interaction of extremophilic organisms with electrodes. To this end, the available knowledge on pure cultures of extremophilic microorganisms has been compiled and the study has been extended with the help of bioinformatic analyses on the potential distribution of different electron transfer mechanisms in extremophilic microorganisms.

show abstract

Section: Extremophilic Organisms Thriving On Cathodesmentioning

confidence: 99%

Electron transfer of extremophiles in bioelectrochemical systems

et al. 2022

View full text Add to dashboard Cite

show abstract

“…The copyright holder for this this version posted November 8, 2022. ; https://doi.org/10.1101/2022.09.12.507646 doi: bioRxiv preprint molecular details of extracellular electron uptake (EET) from a cathode are also subject to keen debate with regard to K. spormannii (Jung et al, 2020;Pillot et al, 2022a;Prévoteau et al, 2020;Reiner et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

“…While -with respect to the inanimate components of a MES system -much effort has been put into studying the effect of material and structural properties of electrodes, the development of new and, in particular, scalable reactor concepts for MES has been rather sidelined (Enzmann et al, 2018;Guo et al, 2015;Kerzenmacher, 2017;Pillot et al, 2022aPillot et al, , 2022bZhang et al, 2021). This is in spite of the fact that whether microbial electrochemical technologies (METs) can carve a niche in a future sustainable economy depends strongly on the availability of a suitable reactor technology.…”

Section: Introductionmentioning

confidence: 99%

A scalable, rotating disc bioelectrochemical reactor (RDBER) suitable for the cultivation of both cathodic and anodic biofilms

Hackbarth

Gescher

Horn

et al. 2023

Bioresource Technology Reports

View full text Add to dashboard Cite

“…While -with respect to the inanimate components of a MES system -much effort has been put into studying the effect of material and structural properties of electrodes, the development of new and, in particular, scalable reactor concepts for MES has been rather sidelined (Enzmann et al, 2019b;Guo et al, 2015;Kerzenmacher, 2017;Pillot et al, 2022aPillot et al, , 2022bZhang et al, 2021). This is in spite of the fact that whether microbial electrochemical technologies (METs) can carve a niche in a future sustainable economy depends strongly on the availability of a suitable reactor technology.…”

Section: Introductionmentioning

confidence: 99%

“…Thus, it was shown that K. spormannii forms robust biofilms with a thickness of well over 100 μm – an important prerequisite for MES as stated recently by Vassilev and colleagues (Barham et al, 1984; Hackbarth et al, 2020; Vassilev et al, 2022). Although it seems to be an intrinsic property of electroautotrophic organisms, the molecular details of extracellular electron uptake (EET) from a cathode are also subject to keen debate with regard to K. spormannii (Jung et al, 2020; Pillot et al, 2022a; Prévoteau et al, 2020; Reiner et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

A scalable, Rotating Disc Bioelectrochemical Reactor (RDBER) suitable for the cultivation of both cathodic and anodic biofilms

Hackbarth¹,

Gescher

Horn³

et al. 2022

Preprint

View full text Add to dashboard Cite

The question of whether microbial electrochemical technologies can carve a niche in a future sustainable economy will strongly depend on the availability of suitable reactor technology. However, examples of new, scalable reactor concepts or even scale-ups specifically for microbial electrosynthesis applications are rarely described in the literature. Here, we present a membrane-less reactor system suitable for different bioelectrochemical applications. The system employs rotating graphite disks with a diameter of 210 mm as working electrodes with a total surface area of 1 m2. Given the reactor volume of 10 L, this results in a surface-to-volume ratio of 100:1 m2 m-3. Moreover, this rotating disk bioelectrochemical reactor (RDBER) is fully autoclavable, pressurizable to at least 0.5 bars and an optical window allows for the in vivo observation of biofilm development on the electrodes by means of optical coherence tomography (OCT). As a proof-of-principle for an aerobic microbial electrosynthesis process, we cultivated the thermoacidophilic, electroautotrophic bacterium Kyrpidia spormannii in the RDBER and investigated the spatial distribution of the cathodic biofilm on the rotating working electrode by OCT. Thereby, biofilm heights of more than 75 μm were achieved. The initial cell density seems to be crucial for successful K. spormannii biofilm formation. To demonstrate the versatility of the system, we further operated the reactor anodically as a microbial electrolysis cell. For this purpose, we inoculated the RDBER with a co-culture of Shewanella oneidensis and Geobacter sulfurreducens in acetate- and lactate-containing medium at a working electrode potential of 0 mV vs. SHE. During this anodic batch operation, current densities of up to 130 μA cm-2 could be achieved. Furthermore, a correlation between the biofilm accumulation rate, current density and hydrogen production as well as substrate degradation rate was observed. These cultivation experiments with different electroactive microorganisms not only prove the basic functionality of the RDBER, but also illustrate that the RDBER can satisfy a wide range of biotechnological requirements for pure culture applications.

show abstract

Effect of cathode properties on the thermophilic electrosynthesis of PolyHydroxyAlkanoates by Kyrpidia spormannii

Cited by 4 publications

References 32 publications

Electron transfer of extremophiles in bioelectrochemical systems

Electron transfer of extremophiles in bioelectrochemical systems

A scalable, rotating disc bioelectrochemical reactor (RDBER) suitable for the cultivation of both cathodic and anodic biofilms

A scalable, Rotating Disc Bioelectrochemical Reactor (RDBER) suitable for the cultivation of both cathodic and anodic biofilms

Contact Info

Product

Resources

About