Effect of Chronic Iodide Supplements on Thyroid Secretion: The Role of Lysosomes*

Lamas, Luis; Couvreur, Michel; Obregón, Marı́a Jesús; Hove-Vandenbroucke, Marie-France Van Den

doi:10.1210/endo-110-5-1804

Cited by 16 publications

(4 citation statements)

References 27 publications

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“…Homogenates were centrifuged at 400 g for 10 min, at 30 000 g for 10 min and at 134 000 g for 60 min to isolate, respectively, pellet CN (cell debris and nuclei), ML (crude lysosomal fraction), P (microsomes) and the final supernatant (S) (Lamas et al 1982). 125 I in the homogenate and in each fraction was measured.…”

Section: Analytical Subcellular Fractionationmentioning

confidence: 99%

“…Solubilized 125 I was layered on 5±20% sucrose gradients and centrifuged as previously reported (van den Hove et al 1995). 19S thyroglobulin (Tg) was purified from supernatants as described (van den Hove et al 1982). Tg concentration was calculated from absorbance at 280 nm, using a specific absorption coefficient of 10.0 cm ±1 for a Tg solution of 10 mg/ml.…”

Section: Sucrose Gradient Centrifugation Of Iodocompoundsmentioning

confidence: 99%

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Age delays thyroglobulin progression towards dense lysosomes in the cream hamster thyroid

Hove

Couvreur

Authelet

et al. 1998

Cell and Tissue Research

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We have shown that large lysosomes appear in thyroids of aging male cream hamsters. To investigate the role of this lysosomal change in the age-dependent reduction in hormone secretion, thyroids of young (<4 months of age) and old (>22 months of age) male and female hamsters were labeled with 125I at near isotopic equilibrium. Changes in thyroid morphology were analyzed by light- and electron-microscopic morphometry. Changes in thyroglobulin processing were analyzed by subcellular fractionation and identification of 125I-compounds by sucrose gradients and reverse-phase high-pressure liquid chromatography (HPLC). Sexual dimorphism present in thyroids of young animals became more marked upon aging. The parallel increase in thyroid weight and thyroglobulin content was more conspicuous in old females than in old males. Two morphological observations were specific to old females: (1) large follicles with flat epithelium and evenly labeled colloid and (2) deposits of amyloid material (possibly immunoglobulin light chain-related) between follicles. Although lysosomes were enlarged in female and male aged thyroids, they did not accumulate iodine. However, after isopycnic centrifugation of crude lysosomal fractions in Percoll gradients, 125I in old thyroids was not distributed mainly in the dense fraction L1 (lysosomes) as in young thyroids, but partly in particles of lower density (light L2 and buoyant fractions). 125I in the lighter particles was mostly found in intact thyroglobulin and in large iodopeptides. This 125I shift towards less dense particles was more marked in females than in males. These results indicate that age delays thyroglobulin progression towards dense lysosomes and suggest that the slower traffic of thyroglobulin in the endocytic pathway contributes to the reduction in thyroid hormone secretion in the aged cream hamster.

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Section: Analytical Subcellular Fractionationmentioning

confidence: 99%

Section: Sucrose Gradient Centrifugation Of Iodocompoundsmentioning

confidence: 99%

Age delays thyroglobulin progression towards dense lysosomes in the cream hamster thyroid

Hove

Couvreur

Authelet

et al. 1998

Cell and Tissue Research

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“…The high protease activities in cold adenomas and adenocarcinomas were not associated with the increased secretion of thyroid hormones, but seemed to reflect the enhanced turnover of intracellular proteins (Starling et al, 1981), as it is found in other neoplastic cells (Dzialoszynski et al, 1966). The existence of such heterogenous population of lysosomes in thyroid glands was suggested in the recovery phase of low iodine diet fed rats after refeeding with an iodine sufficient diet (Lamas et al, 1982), although we could not provide more findings to demonstrate the heterogenous population of lysosomes.…”

Section: Discussionmentioning

confidence: 71%

Acid Thyroglobulin Protease Activities in Human Diseased Thyroid Glands

Yoshinari

Okamura

Shiroozu

et al. 1987

Clinical Endocrinology

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The lysosomal acid protease is thought to be a main enzyme in hydrolysis of thyroglobulin. The protease activity of lysosome-rich fractions of various diseased thyroid glands were assayed by the sensitive and reproducible method developed in our laboratory. They included 78 diseased thyroid glands; 37 thyrotoxic goitres, five irradiated thyroids, five Hashimoto's thyroiditis, one granulomatous thyroiditis, six multinodular goitres, 14 'cold' adenomas, four 'hot' adenomas with three paranodular tissues, and six carcinomas. Fifteen paranodular tissues of cold and solitary thyroid nodules were served as controls. The protease activities were significantly increased in thyroid tissues of thyrotoxic goitres, Hashimoto's thyroiditis, adenomas and adenocarcinomas. In thyrotoxic goitres, the protease activity correlated well with the mean area size of colloid follicles. The 'hot' adenomas had higher protease activity than 'cold' ones. The protease activity of multinodular goitres was nearly normal. Only the irradiated thyroid glands had low protease activities. Therefore the high protease activities in hyperfunctioning goitres seem to reflect hormone secretory activities. On the other hand, cold neoplastic goitres seem to have lysosomal protease activities which are not associated with thyroidal secretory process. The assay of acid protease activity will be useful to understand the pathophysiology of lysosomal system in diseased thyroid glands.

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“…Γ. Σύμφωνα με έναν άλλο μηχανισμό, μεγάλες δόσεις ιωδίου μπορούν να προκαλέσουν νέκρωση των θυρεοειδών αδένων φυσιολογικών ζώων, όπως ινδικών χοιριδίων (Follis 1964), σκύλων (Belshaw and Becker, 1973), ποντικιών (Mahmoud et al, 1986), αρουραίων (Lamas et al, 1982;Contempre et al, 1993). Όταν η δράση της ΤΡΟ εμποδίζεται δεν προκαλείται βλάβη, υποδηλώνοντας ότι ο μηχανισμός της βλάβης από ιώδιο γίνεται μέσω οξείδωσης.…”

Section: ο ρόλος του ιωδίου στην πειραματική θυρεοειδίτιδαunclassified

Κλινικές Μορφολογικές Και Ανοσολογικές Μελέτες Μετά Τη Χορήγηση Ιωδιωμένου Ελαίου Σε Ασθενείς Με Μη Τοξική Βρογχοκήλη

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Effect of Chronic Iodide Supplements on Thyroid Secretion: The Role of Lysosomes*

Cited by 16 publications

References 27 publications

Age delays thyroglobulin progression towards dense lysosomes in the cream hamster thyroid

Age delays thyroglobulin progression towards dense lysosomes in the cream hamster thyroid

Acid Thyroglobulin Protease Activities in Human Diseased Thyroid Glands

Κλινικές Μορφολογικές Και Ανοσολογικές Μελέτες Μετά Τη Χορήγηση Ιωδιωμένου Ελαίου Σε Ασθενείς Με Μη Τοξική Βρογχοκήλη

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