Effect of chronic restraint stress on dendritic spines and excrescences of hippocampal CA3 pyramidal neurons—a quantitative study

Sunanda,; Rao, Muddanna S.; Raju, T.R.

doi:10.1016/0006-8993(95)00822-8

Cited by 131 publications

(81 citation statements)

References 31 publications

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“…Acute or chronic physical stress also alters spine densities in adult hippocampal neurons, where elevated spine densities have been found in rat hippocampal CA1 pyramidal neurons after handling young rats during saline injections, a manipulation that is associated with stress and even painful sensation (50). Chronic restraint stress induces increased spine densities in CA3 hippocampal pyramidal cells (51), whereas acute tail shock results in increased spine densities on the apical and basal dendrites of CA1 pyramidal neurons (52).…”

Section: Discussionmentioning

confidence: 99%

Juvenile emotional experience alters synaptic composition in the rodent cortex, hippocampus, and lateral amygdala

Poeggel

Helmeke

Abraham

et al. 2003

Proc. Natl. Acad. Sci. U.S.A.

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A quantitative anatomical study in the rodent anterior cingulate and somatosensory cortex, hippocampus, and lateral amygdala revealed region-, cell-, and dendrite-specific changes of spine densities in 3-week-old Octodon degus after repeated parental separation. In parentally separated animals significantly higher spine densities were found on the apical and basal dendrites of the cingulate cortex (up to 143% on apical and 138% on basal dendrite). Branching order analysis revealed that this effect is seen on all segments of the apical dendrite, whereas on the basal dendrites significantly higher spine densities were seen only on the outer branches (third to fifth dendritic segments). Increased spine densities were also observed on the hippocampal CA1 pyramidal neurons (up to 109% on the distal apical segments and up to 106% on the basal segment) compared with the control group. In contrast, significantly reduced spine densities were observed on the granule cell dendrites in the dentate gyrus (down to 92%) and on the apical dendrites in the medial nucleus of the amygdala (down to 95%). No significant changes of spine densities were seen in the somatosensory cortex (except for an increase in the proximal apical segments) and in the lateral nucleus of the dorsal amygdala (except for an increase in the proximal basal dendritic segments). These results demonstrate that repeated stressful emotional experience alters the balance of presumably excitatory synaptic inputs of pyramidal neurons in the limbic system. Such experience-induced modulations of limbic circuits may determine psychosocial and cognitive capacities during later life.limbic system ͉ stress ͉ dendritic spines ͉ parental separation ͉ cingulate cortex

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Section: Discussionmentioning

confidence: 99%

Juvenile emotional experience alters synaptic composition in the rodent cortex, hippocampus, and lateral amygdala

Poeggel

Helmeke

Abraham

et al. 2003

Proc. Natl. Acad. Sci. U.S.A.

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“…For instance, corticosterone secretion induced by stressors alters dendritic branching (16,40), dendritic spine density (41,42), neurogenesis (43), and synaptic plasticity (44,45) even after a single exposure to stress (42,44). The prepubertal brain may be more sensitive than the adult brain to corticosterone, because an equivalent dose of corticosterone increased hippocampal N-methyl-d-aspartate receptor subunit expression (e.g.…”

Section: Pubertal Modulation Of Experience-dependent Changes In Hormomentioning

confidence: 99%

Stress History and Pubertal Development Interact to Shape Hypothalamic-Pituitary-Adrenal Axis Plasticity

et al. 2006

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Both the magnitude and the duration of the hormonal stress response change dramatically during neonatal development and aging as well as with prior experience with a stressor. However, surprisingly little is known with regard to how pubertal maturation and experience with stress interact to affect hypothalamic-pituitary-adrenal axis responsiveness. Because adolescence is a period of neurodevelopmental vulnerabilities and opportunities that may be especially sensitive to stress, it is imperative to more fully understand these interactions. Thus, we examined hormonal and neural responses in prepubertal (28 d of age) and adult (77 d of age) male rats after exposure to acute (30 min) or more chronic (30 min/d for 7 d) restraint stress. We report here that after acute stress, prepubertal males exhibited a significantly prolonged hormonal stress response (e.g. ACTH and total and free corticosterone) compared with adults. In contrast, after chronic stress, prepubertal males exhibited a higher response immediately after the stressor, but a faster return to baseline, compared with adults. Additionally, we demonstrate that this differential stress reactivity is associated with differential neuronal activation in the paraventricular nucleus of the hypothalamus, as measured by FOS immunohistochemistry. Using triple-label immunofluorescence histochemistry, we found that a larger proportion of CRH, but not arginine vasopressin, cells are activated in the arginine vasopressin in response to both acute and chronic stress in prepubertal animals compared with adults. These data indicate that experience-dependent plasticity of the hypothalamic-pituitary-adrenal neuroendocrine axis is significantly influenced by pubertal maturation. (Endocrinology 147: 1664 -1674, 2006)

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“…Three weeks of restraint stress induces shrinkage of the apical dendrites of CA3 pyramidal neurons [18,31,34,35] and Fuchs et al ([10], unpublished observations) observed similar alterations in the hippocampus of the tree shrew. Since the atrophy is a reversible phenomenon ( [17], unpublished observations, [ 18] unpublished observations) it is not likely that the dendritic atrophy reflects early signs of neuron loss.…”

mentioning

confidence: 84%

“…This is supported by findings of Luine et al [17] who reported that spatial deficits following three weeks of stress, which were accompanied with the dendritic atrophy, was also reversible. Furthermore Sunanda Roa and Raju [31] reported that the stress-induced shrinkage of the apical tree of CA3 pyramidal neurons was accompanied with an increased number of dendritic spines of apical and basal dendrites. In line with this we did not observe degenerative changes in the hippocampus following eight days of subordination stress at present using a sensitive marker for degenerative changes [13,22,32].…”

mentioning

confidence: 99%

“…Longer periods of exposure to corticosteroids may cause loss of hippocampal neurons [25] (but see also [2]) and shorter exposure to corticosterone increases the vulnerability of hippocampal neurons [9,26,28,29]. Similarly, three weeks of restraint stress causes shrinkage of the apical tree of hippocampal CA3 pyramidal neurons in rats [18,31,34,35], exposure to longer periods of stress may cause hippocampal neuron loss [21,33] and shorter periods of stress appear to endanger hippocampal neurons [30].…”

mentioning

confidence: 99%

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Prolonged subordination stress increases Calbindin-D28k immunoreactivity in the rat hippocampal CA1 area

et al. 1996

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Previously we observed that corticosteroids alter Calbindin-D28k immunoreactivity in the rat hippocampus. In the present study we investigated whether prolonged subordination stress, presumably producing elevated plasma corticosterone levels (I) altered the immunocytochemical distribution of the CaZ+-binding proteins Calbindin-D28k (CBir) and Parvalbumin (PVir) in the rat hippocampus. and (2) induced ongoing neurodegenerative changes using a silver impregnation method. Eight days of subordination stress reduced body weight, increased adrenal weight corrected for body weight and reduced thymus weight, indicating its effectiveness to produce a stressful situation. Stress increased CBir selectively in the CAI pyramidal cell layer whereas PVir was not altered. Silver-impregnation revealed no ongoing neurodegenerative changes in any of the hippocampal subfields.

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Effect of chronic restraint stress on dendritic spines and excrescences of hippocampal CA3 pyramidal neurons—a quantitative study

Cited by 131 publications

References 31 publications

Juvenile emotional experience alters synaptic composition in the rodent cortex, hippocampus, and lateral amygdala

Juvenile emotional experience alters synaptic composition in the rodent cortex, hippocampus, and lateral amygdala

Stress History and Pubertal Development Interact to Shape Hypothalamic-Pituitary-Adrenal Axis Plasticity

Prolonged subordination stress increases Calbindin-D28k immunoreactivity in the rat hippocampal CA1 area

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