Effect of Chronic Unpredictable Mild Stress on Adrenal Cortex of Adult Rat and The Possible Protective Role of Licorice Extract: A Histological and Immunohistochemical study

Sadek, Mona Tayssir; El-Abd, Shereen S; Ibrahim, Marwa A.

doi:10.21608/ejh.2020.45532.1369

Cited by 3 publications

(9 citation statements)

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“…In a line with the previous structural changes, immunohistochemical study in the current work demonstrated enormous increase of caspase 3 positive cells in the depression group in comparison to other groups. Sadek et al (2021) who observed similar results in rats exposed to chronic unpredictable mild stress protocol explained that caspases are a family of cysteine protease enzymes that act as death effector molecules in apoptosis, and proposed that chronic stress might lead to activation of certain processes that would end in cell apoptosis through activation of caspases.…”

Section: Advances In Animal Andmentioning

confidence: 72%

Effect of Experimentally Induced Depression on the Adrenal Cortex of Adult Male Albino Rats and the Possible Ameliorative Role of Voluntary Exercise

Eldomiaty¹,

Elsawaf²,

Ali³

et al. 2023

AAVS

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This study was designed to investigate the changes that occur in adrenal cortex in an animal model of depression and the effect of voluntary exercise on these changes. Thirty-two adults male Wistar rats were included based on their forced swimming test behavior. The rats were divided into 4 groups. Group 1 was the control group, group II was the control exercise group, group III was the depression group, and group IV was the depression exercise group. The forced swimming protocol was used to induce depression, while the rat voluntary wheel was used for voluntary exercise. After scarification, estimation of corticosterone level was conducted, and samples of adrenal gland were examined for structural changes by light and Electron microscope, and for immunohistochemical expression. Rats from group III showed statistically increased corticosterone level and increased cortical thickness compared to other groups. Voluntary exercise improved these measures in rats of group IV. Histological disorganization of cortical cells was observed in group III with manifest large cytoplasmic vacuoles. Enormous increase in the number of both apoptotic and proliferative cells was seen with significantly higher mean number of the proliferative cells. By EM, the nuclei were shrunken, secretory vesicles were depleted, and the smooth endoplasmic reticulum showed massive dilatation. These findings were partially restored in rats of group IV. This study concluded that voluntary exercise enhanced recovery from the injurious effect of depression on adrenal cortical cells that partially showed regain of their normal structure and corticosterone secretion.

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Section: Advances In Animal Andmentioning

confidence: 72%

Effect of Experimentally Induced Depression on the Adrenal Cortex of Adult Male Albino Rats and the Possible Ameliorative Role of Voluntary Exercise

Eldomiaty¹,

Elsawaf²,

Ali³

et al. 2023

AAVS

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“…(2015) , Zhang et al . (2019) and Sadek et al . (2021) showing that stressed dams had greater levels of plasma corticosterone than control dams.…”

Section: Resultsmentioning

confidence: 97%

“…Previous research has demonstrated that high cortisol levels have a negative feedback effect on the production of corticotropin-releasing hormone (CRH) from the hypothalamus, causing the decrease of corticosterone release from the adrenal cortex ( Allen & Sharma 2021 ). However, with prolonged stress, that negative feedback fails to limit cortisol release, and its levels rise to dangerously high levels, eventually resulting in neuroendocrine, cardiovascular and immunological diseases ( Sadek et al . 2021 ).…”

Section: Resultsmentioning

confidence: 99%

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Effect of Bee Bread on Corticosterone Level in Rat Dams Exposed to Gestational Heat Stress

Nur Akmar Nadhirah Mohd Nor,

Asmad Kari,

Mohd Nizam Haron

et al. 2023

tlsr

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Environmental temperature rises are powerful stimuli that can alter both the sympathetic nervous system and the hypothalamic-pituitary-adrenocortical axis (HPA). Heat stress has been shown to harm pregnancy outcomes such as causing spontaneous abortion, low birth weight, growth retardation and stillbirth. Supplementation of bee bread in pregnant rats under heat stress exposure has been shown to improve the pregnancy outcomes. However, whether supplementation of bee bread during heat stress exposure may also reduce the level of the stress hormone, corticosterone has yet been reported. Therefore, this study aims to determine the effect of bee bread on corticosterone level, progesterone level, oestradiol level and zonation of the adrenal cortex of pregnant rats under heat stress exposure. Pregnant rats were randomly categorised into four groups (n = 6): Control (C: standard feeding), Treatment 1 (T1: 0.5 g bee bread/kg body weight/ day), Treatment 2 (T2: standard feeding with heat exposure), and Treatment 3 (T3: 0.5 g bee bread/kg body weight/day with heat exposure). Bee bread (0.5 g/kg body weight/ day) was force-fed to pregnant rats through oral gavage beginning on day 0 of pregnancy and continuing until delivery. Heat stress was generated experimentally by putting both T2 and T3 rats in an egg incubator for 45 min daily at a temperature of 43°C till delivery. On a postnatal Day 21, dams were euthanised to assess serum corticosterone, progesterone, oestradiol levels and adrenal gland histology. Rats in the T2 group had a significantly (P < 0.05) increase in the zona fasciculata thickness (94.95 ± 1.55 ?m) and higher corticosterone levels (49.57 ± 1.57 ng/mL) compared with control. However, supplementation of bee bread during heat stress was able to show an improvement in adrenal zona fasciculata thickness by decreasing to 79.89 ± 3.08 ?m and corticosterone level reduced to 35.31 ± 1.73 ng/mL significantly (P < 0.05). Therefore, these findings may imply that bee bread is effective as a neutralizer in lowering the production of stress hormone.

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“…The cytokines such as TNF-∝ stimulate collagen deposition (Pratt & Kaplan, 2001). Thickening of the adrenal capsule was demonstrated by many researchers in stressed rats (Sadek et al, 2021 andZaki et al, 2018). The suprarenal capsule is a signaling center that controls cell zonation and renewal by the capsular enzymes that imprint the fate of glomerulosa cells (Vidal et al, 2016).…”

Section: Ii-morphometric Resultsmentioning

confidence: 99%

Effect of Roundup on the Suprarenal Cortex in the Adult Male Albino Rat and the Possible Ameliorative Role of Gallic Acid. Histological and Immunohistochemical study

Shenouda

2023

Egyptian Academic Journal of Biological Sciences, D. Histology

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INTRODUCTIONThe suprarenal cortex consists of three distinctive zones, each having an imperative function. The zona glomerulosa secretes aldosterone, which modulates body electrolyte balance, the zona fasciculata, secretes glucocorticoids which modulate immune and metabolic functions and the zona reticularis that produce androgens (Merlo, et al., 2016) The herbicide, Roundup consists of glyphosate, as an active ingredient, and the surfactant, polyoxyethyleneamine, (Olah, et al.,2022). Owing to its high-water solubility, it is considered a broad-spectrum herbicide that is broadly used. It controls weeds, emerged grasses and cultures for example soy, corn and rice (Williams et al.,2000).

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Effect of Chronic Unpredictable Mild Stress on Adrenal Cortex of Adult Rat and The Possible Protective Role of Licorice Extract: A Histological and Immunohistochemical study

Cited by 3 publications

References 0 publications

Effect of Experimentally Induced Depression on the Adrenal Cortex of Adult Male Albino Rats and the Possible Ameliorative Role of Voluntary Exercise

Effect of Experimentally Induced Depression on the Adrenal Cortex of Adult Male Albino Rats and the Possible Ameliorative Role of Voluntary Exercise

Effect of Bee Bread on Corticosterone Level in Rat Dams Exposed to Gestational Heat Stress

Effect of Roundup on the Suprarenal Cortex in the Adult Male Albino Rat and the Possible Ameliorative Role of Gallic Acid. Histological and Immunohistochemical study

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