Effect of cold temperature on the composition of different lipid classes of the foodborne pathogen Listeria monocytogenes: Focus on neutral lipids

Mastronicolis, Sofia K.; Boura, A.; Karaliota, Alexandra; Magiatis, Prokopios; Arvanitis, Nikolaos; Litos, Charalambos; Tsakirakis, Angelos; Paraskevas, P.; Moustaka, H.; Heropoulos, Georgios A.

doi:10.1016/j.fm.2005.03.001

Cited by 26 publications

(40 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…1B and A, respectively). As expected from previous reports, 16 the major detected components of the L. monocytogenes membrane bilayer were phosphatidylglycerol, cardiolipin and their lysine-modified forms. At both 30 °C and 37 °C, LysPG made up the highest proportion (54% and 73%, respectively) of the total lipid composition at early stages of growth while cardiolipin increased during progression into early stationary phase (Fig.…”

Section: Membrane Lipid Composition Changes With Growth Phase and Temsupporting

confidence: 90%

“…Studies characterizing the effect of cold shock on L. monocytogenes have shown that reduction in growth temperature from 30 °C to 6 °C is correlated with an increase in the proportion of neutral lipids and branched chain fatty acids in the cell membrane as well as a decrease in overall lipid chain length. 15,16 Since a cell wall stress regulator, VirR, 7 controls LysPGS production in L. monocytogenes, we decided to investigate whether there was also 32 P] PPi is represented as a measure of optical density by the black line. aliquots of bacterial culture were removed and used for lipid isolation and subsequent separation by TLc.…”

Section: Membrane Lipid Composition Changes With Growth Phase and Temmentioning

confidence: 99%

See 1 more Smart Citation

LysPGS formation inListeria monocytogeneshas broad roles in maintaining membrane integrity beyond antimicrobial peptide resistance

et al. 2014

View full text Add to dashboard Cite

Section: Membrane Lipid Composition Changes With Growth Phase and Temsupporting

confidence: 90%

Section: Membrane Lipid Composition Changes With Growth Phase and Temmentioning

confidence: 99%

LysPGS formation inListeria monocytogeneshas broad roles in maintaining membrane integrity beyond antimicrobial peptide resistance

et al. 2014

View full text Add to dashboard Cite

“…pH stress did not induce gross changes in the total amounts of unsaturated FAs. This is interesting because pH stress induces considerable changes in total unsaturated FA content in other bacteria, and L. monocytogenes does make such changes in response to other environmental stresses (4,13,25,26,29,32,49). The absence of significant differences between the responses of L. monocytogenes 10403S and the isogenic sigB null mutant suggests that sigB does not have a major role in pH-induced fatty acid modulation in L. monocytogenes.…”

mentioning

confidence: 99%

Role of Branched-Chain Fatty Acids in pH Stress Tolerance in Listeria monocytogenes

Giotis

McDowell

Blair

et al. 2007

Appl Environ Microbiol

128

View full text Add to dashboard Cite

In alkaline conditions, Listeria monocytogenes cells develop higher proportions of branched-chain fatty acids (FAs), including more anteiso forms. In acid conditions, the opposite occurs. Reduced growth of pH-sensitive mutants at adverse pH (5.0/9.0) was alleviated by the addition of 2-methylbutyrate (an anteiso-FA precursor), suggesting that anteiso-FAs are important in adaptation to adverse pH. The balance between anteiso-and iso-FAs may be more important than changes in the amounts and/or degrees of saturation of FAs in pH adaptation.Listeria monocytogenes can grow under a wide range of pH stress, i.e., 4.1 to 9.0 (38, 46), increasing its abilities to persist during food processing and attempts to decontaminate food-processing environments (15,(42)(43)(44)(45). It also has particularly impressive capacities to modulate its membrane lipids to maintain membrane fluidity and transport functions (10,40,41) in response to temperature (1), salt (7), and CO 2 /anaerobiotic (21) stress. Such capacities have been suggested to be related to its atypically high iso and anteiso, odd-numbered, branched-chain fatty acid (BCFA) content (1,20) and its ability to modulate the overall content and proportions of BCFAs, straight-chain FAs (SCFAs), and unsaturated FAs (22,23). For example, reductions in environmental temperatures lead to increases in the amount of ai15:0 present in L. monocytogenes cell membranes, while increases in environmental temperatures lead to reductions in the amounts of ai15:0 and other BCFAs present in membranes (1,14,32).Changes in FA profile have been associated with pH adaptation in Streptococcus mutans (16-18), Escherichia coli (5, 48), and Salmonella (24), Pseudomonas (31), and Bacillus species (23). However, little is known about pH stress-associated modulation of FAs in L. monocytogenes (21), the wider role of FA modulation in its responses to nonthermal stresses, or the cross-protection mechanisms which operate in this hardy pathogen (19,27,35,44).The aims of this study were to investigate the modulation of the FA profile of L. monocytogenes membranes in response to changes in environmental pH, investigate the effects of an exogenous BCFA precursor on the pH stress response of BCFA-deficient mutants (1, 49), and examine possible links between the prevalence of anteiso-BCFAs and the adaptation mechanism(s) of L. monocytogenes under adverse pH conditions. Modified brain heart infusion broth (MBHIB; Difco Laboratories, Sparks, MD), suitable for adverse-pH studies, was prepared to pH 5.0, 5.5, and 6.0 in 2 M disodium phosphate Washed cells from mid-exponential-phase cultures of L. monocytogenes 10403S, an isogenic sigB null mutant (3), and isogenic non-BCFA-producing cld-1 and cld-2 mutants (1, 49) were inoculated into preheated (30°C) 100-ml volumes of the buffered MBHIB and grown (30°C/200 rpm) to an optical density at 600 nm of 0.5 to 0.6. Growth rates of cultures (doubling times per hour of cultures in exponential growth) were calculated (2). Mid-exponential-phase cells were recovered by centrif...

show abstract

“…These cause an increase in the amount of odd-numbered fatty acids and branched-chain fatty acids, a switch from iso to anteiso branching patterns, and, later, a shortening of the length of the branched-chain fatty acids (1,19,20,25). These changes lead to maintenance of constant membrane fluidity at low temperatures by including fatty acids with lower phase transition temperatures (Tc) (12,18,20). Several studies have used coldsensitive (1,8) and nisin-resistant (7,33) mutants to better understand the changes that lead to their phenotypes.…”

mentioning

confidence: 99%

“…Several authors have examined changes in phospholipid composition to evaluate membrane fluidity at various temperatures (18,20,25,33). The present research uses static and real-time measurements to better understand the role of membrane fluidity in the stress resistance and sensitivity phenotypes of the two mutants.…”

mentioning

confidence: 99%

Changes in Listeria monocytogenes Membrane Fluidity in Response to Temperature Stress

Najjar

Chikindas

Montville

2007

Appl Environ Microbiol

View full text Add to dashboard Cite

Listeria monocytogenes is a food-borne pathogen that has been implicated in many outbreaks associated with ready-to-eat products. Listeria adjusts to various stresses by adjusting its membrane fluidity, increasing the uptake of osmoprotectants and cryoprotectants, and activating the B stress factor. The present work examines the regulation of membrane fluidity through direct measurement based on fluorescent anisotropy. The membrane fluidities of L. monocytogenes Scott A, NR30, wt10403S, and cld1 cells cultured at 15 and 30°C were measured at 15 and 30°C. The membrane of the cold-sensitive mutant (cld1) was more rigid than the membranes of the other strains when grown at 30°C, but when grown at 15°C, it was able to adjust its membrane to approach the rigidity of the other strains. The difference in rigidities, as determined at 15 and 30°C, was greater in liposomes than in whole cells. The rates of fluidity adjustment and times required for whole cells to adjust to a different temperature were similar among strains but different from those of liposomes. This suggests that the cells had a mechanism for homeoviscous adaptation that was absent in liposomes.

show abstract

Effect of cold temperature on the composition of different lipid classes of the foodborne pathogen Listeria monocytogenes: Focus on neutral lipids

Cited by 26 publications

References 36 publications

LysPGS formation inListeria monocytogeneshas broad roles in maintaining membrane integrity beyond antimicrobial peptide resistance

LysPGS formation inListeria monocytogeneshas broad roles in maintaining membrane integrity beyond antimicrobial peptide resistance

Role of Branched-Chain Fatty Acids in pH Stress Tolerance in Listeria monocytogenes

Changes in Listeria monocytogenes Membrane Fluidity in Response to Temperature Stress

Contact Info

Product

Resources

About