Effect of Cold Temperature on Shoot Regeneration in Vitro from Aged Cultures of GF-677 (Prunus Persica × Prunus Amygdalus)

Dimasi-Theriou, Kortessa; Economou, A. S.

doi:10.1007/978-1-4684-5760-5_43

Cited by 3 publications

(1 citation statement)

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“…Similar paradoxical observations were made for temperature effects, as both a 3-day cold pretreatment and incubation at high temperatures can increase callus formation in Arabidopsis , indicating the existence of an optimum for every system [ 44 , 158 ]. Extended cold treatments of 3–5 weeks at 3 °C could also overcome growth arrest in subcultures of crosses between peach and almond trees [ 163 ]. Optimal conditions for osmotic-stress-induced somatic embryogenesis from shoot tips and leaf buds in Arabidopsis differed between ecotypes [ 13 ], and the addition of sorbitol to the culture medium promoted shoot organogenesis from seed-derived callus in rice [ 164 ].…”

Section: Other Sources Of Regenerative Variationmentioning

confidence: 99%

Natural Variation in Plant Pluripotency and Regeneration

Lardon

Geelen

2020

Plants

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Plant regeneration is essential for survival upon wounding and is, hence, considered to be a strong natural selective trait. The capacity of plant tissues to regenerate in vitro, however, varies substantially between and within species and depends on the applied incubation conditions. Insight into the genetic factors underlying this variation may help to improve numerous biotechnological applications that exploit in vitro regeneration. Here, we review the state of the art on the molecular framework of de novo shoot organogenesis from root explants in Arabidopsis, which is a complex process controlled by multiple quantitative trait loci of various effect sizes. Two types of factors are distinguished that contribute to natural regenerative variation: master regulators that are conserved in all experimental systems (e.g., WUSCHEL and related homeobox genes) and conditional regulators whose relative role depends on the explant and the incubation settings. We further elaborate on epigenetic variation and protocol variables that likely contribute to differential explant responsivity within species and conclude that in vitro shoot organogenesis occurs at the intersection between (epi) genetics, endogenous hormone levels, and environmental influences.

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