Effect of cryopreservation on sperm motility parameters and fertilizing ability of brown trout semen

Nynca, Joanna; Dietrich, G.J.; Dobosz, Stefan; Grudniewska, Joanna; Ciereszko, Andrzej

doi:10.1016/j.aquaculture.2014.05.037

Cited by 64 publications

(42 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Semen analysis using CASA has been reported in other species of fish and has been shown to be a good tool to help determine sperm quality; it provides more information than subjective analysis and more accurate results (NYNCA et al, 2014;VIVEIROS et al, 2014;JUDYCKA et al, 2015;MARIA et al, 2015). This is one of the first studies using CASA in the analysis of fresh and cryopreserved sperm for P. brevis.…”

Section: Sperm Cryopreservation and Thawingmentioning

confidence: 92%

Cryopreservation of Prochilodus brevis semen: freezing media and thawing rates

Nunes

Oliveira

Lopes

et al. 2016

SCA

View full text Add to dashboard Cite

Prochilodus brevis is a migratory fish, an important component of its river ecosystem and an appreciated animal in northeastern cuisine. However, human activities have threatened its survival. Thus, researchers have become interested in developing genetic material storage protocols, such as seminal cryopreservation. Therefore, determination of the appropriate freezing media and thawing rate is a fundamental step toward the use of this biotechnology in the production of common curimatã and for reducing risks to the species' survival. As such, the aim of the current study was to evaluate the effect of different freezing media and thawing rates on the quality of cryopreserved semen from P. brevis. For this study, males received a single dose of pituitary extract of carp 18 hours before semen collection. The semen samples were diluted in 5% glucose + 10% methyl glycol (MG), 5% glucose + 10% dimethyl sulfoxide (DMSO), 0.9% NaCl + 10% methyl glycol, and 0.9% NaCl + 10% dimethyl sulfoxide, loaded into 0.25-mL straws and frozen in liquid nitrogen vapor. The semen from each treatment was thawed at three different thawing rates: 25 °C for 30 sec, 30 °C for 16 sec and 40 °C for 12 sec. Motility, vitality and morphology analyses were performed by computer-assisted sperm analysis (CASA). The characteristics of the fresh sperm mostly resembled those found in the literature. For the parameters analyzed, fresh sperm presented higher sperm quality in comparison to all treatments with cryopreserved sperm (p<0.05), except for the characteristic of normal morphology, for which the sperm cryopreserved in glucose and MG did not differ statistically from the fresh sperm. For the cryopreserved semen, the greatest results of total motility and curvilinear velocity (VCL) were obtained using glucose and DMSO, regardless of the thawing rate employed. For the straight-line velocity (VSL) and average path velocity (VAP), DMSO showed the best results, regardless of the diluent and thawing rate. With regard to vitality, the highest values were achieved when DMSO and thawing rates of 30 °C for 16 sec or 40 °C for 12 sec were used. In the morphological analysis, the greatest percentage of normal sperm cells was obtained using thawing rates of 25 °C for 30 sec and 40 °C for 12 sec, regardless of the freezing media. Sperm quality was found to suffer interference from the freezing media, as well as from interaction between its components (diluent and cryoprotectant) and the thawing rate used. Under the methodological conditions employed, the use of 5% glucose + 10% DMSO and a thawing rate of 30 °C for 16 seconds or 40 °C for 12 seconds is recommended for P. brevis semen cryopreservation. ResumoO Prochilodus brevis é um peixe reofílico, importante componente do ecossistema fluvial e apreciado na culinária nordestina. No entanto, ações antrópicas têm ameaçado sua sobrevivência. Desta forma, surge, nos pesquisadores, o interesse no desenvolvimento de protocolos de conservação do material genético, como a criopreservação seminal. Logo, a deter...

show abstract

Section: Sperm Cryopreservation and Thawingmentioning

confidence: 92%

Cryopreservation of Prochilodus brevis semen: freezing media and thawing rates

Nunes

Oliveira

Lopes

et al. 2016

SCA

View full text Add to dashboard Cite

show abstract

“…This method secures good fertilization rates, but a high sperm-to-egg ratio (6.2 Â 10 6 ) is necessary. On the other hand, recent studies shown that storage times up to 15 min did not affect the post-thaw fertility of salmonid semen and even times of 30, 45 and 60 min have been used [2,10]. Thus, the use of lower than previously published sperm-to-egg ratios and also higher post-thaw storage time, leading to high fertilizing ability of Caspian brown trout semen is necessary.…”

mentioning

confidence: 98%

Motility and fertilizing ability of cryopreserved Caspian brown trout (Salmo trutta caspius) sperm: Effect of post-thaw storage time and different sperm-to-egg ratios

et al. 2015

View full text Add to dashboard Cite

“…Glucose is one of the sugars used as external cryoprotectants that stabilize the sperm cell membrane against cryoinjuries [7]. Recently cryopreservation procedures with the use of 0.18 M glucose in 9% methanol were successfully implemented for salmonid fish semen [13,22]. Our results demonstrate that glucose can be an efficient external cryoprotectant for sturgeon spermatozoa as well.…”

Section: Discussionmentioning

confidence: 59%

“…The latter methods employed extenders consisting of potassium ions and sucrose buffered by Tris-HCl in methanol. Simple extenders containing glucose and methanol have been recently used for cryopreservation of salmonid fish sperm [10,13,22]. These results indicate that the buffering of extenders for cryopreservation to specific pH and the addition of potassium ions may not be necessary.…”

mentioning

confidence: 97%